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Kit for detecting cholesterol metabolism abnormity susceptible inheritance risk

A technology for abnormal metabolism and genetic risk, applied in the field of kits for detection of genetic risk of abnormal susceptibility to cholesterol metabolism, which can solve problems such as elevated serum cholesterol and triglycerides

Inactive Publication Date: 2008-08-13
HAINAN ZHUJIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Defects in the APOE gene may lead to elevated serum cholesterol and triglycerides due to the failure of normal clearance of chylomicrons and very low-density lipoproteins, leading to familial beta lipoprotein abnormalities or type III hyperlipoproteinemia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1. Use of detection kits

[0054] Step 1: Extraction of DNA template

[0055] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0056] Step 2: Real-time quantitative PCR reaction

[0057] Use the fluorescent quantitative PCR set in the detection kit, which contains the following primer pairs and fluorescent probe pairs:

[0058] Sense primer 1: 5'-TGTGCAATGGATCAAAATCAG-3' (SEQ ID NO: 1)

[0059] Antisense primer 1: 5'-GTGAGCAAGTCTACTCAC-3' (SEQ ID NO: 2)

[0060] Sense primer 2: 5'-GGCTCACATGAAGGCCAAATTC-3' (SEQ ID NO: 3)

[0061] Antisense primer 2: 5'-AGTTCCTGCTGAATGTCCATTTGAT-3' (SEQ ID NO: 4)

[0062] Sense primer 3: 5'-AATTCAATGTCTCTTC-3' (SEQ ID NO: 5)

[0063] Antisense primer 3: 5'-AGAACAACAACAAAA-3' (SEQ ID NO: 6)

[0064] Sense primer 4: 5'-AGGGTGAGATTCCAAGATAA-3' (SEQ ID NO: 7)

[0065] Antisense primer 4: 5'-CAAGCAAATGACTAAAGAGAA-3' (SEQ ID NO: 8)

[0066] Probe 1 with VIC fluorophore: 5'-ACCAAgGGAGAA-3' (SEQ ID ...

Embodiment 2

[0098] Example 2. Services for genetic risk detection of individual susceptibility to abnormal cholesterol metabolism

[0099] Step 1: DNA Extraction

[0100] The physicians in the laboratory department of the hospital instructed the subjects to use oral swabs to sample oral epithelial cells, and the silica gel adsorption method was used to extract DNA from oral epithelial cells.

[0101] Step 2: Genotyping Assays

[0102] Use the kit provided by the present invention to perform fluorescence quantification on the rs2230806 SNP site on the ABCA1 gene, the rs693 SNP site on the APOB gene, the rs320 SNP site and the rs285 SNP site on the LPL gene For PCR detection, DNA sequencing was performed on the rs429358 SNP site and the rs7412 SNP site on the APOE gene to determine the genotypes of these 6 SNPs sites.

[0103] Step 3: Analysis of Individual Genetic Risk of Susceptibility to Abnormal Cholesterol Metabolism

[0104] Through the analysis of the SNPs genotypes of the tested ...

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PUM

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Abstract

The invention discloses an agent box for detecting individual cholesterin metabolism unconventionality impressionable heredity risks. The agent box comprises specificity primer pair and specificity fluorescent detecting probe pair for detecting number rs2230806 SNP site on adenosine triphosphate bind box transferring body A1 gene (ABCA1), number rs693 SNP site on apolipoprotein B gene (APOB), number rs320 SNP site and rs285 SNP site on lipoproteins lipase gene (LPL), specificity primer pair and DNA sequencing primer for detecting number rs429358 SNP site and rs7412 SNP site on detection apolipoprotein E gene (APOE), fluorescent definite quantity PCR general component, PCR reaction component, PCR outcome yield cleanse component and DNA sequencing reaction component etc.. The agent box of the invention assesses individual cholesterin metabolism unconventionality impressionable heredity risks by detecting synchronously mononucleotide polymorphism site gene type on ABCA1, APOB, LPL and APOE genes correlative closely to individual cholesterin metabolism unconventionality impressionable heredity risks.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine, more specifically, the present invention relates to a kit for detecting the genetic risk of abnormal cholesterol metabolism susceptibility, by simultaneously detecting the adenosine triphosphate binding cassette transporter A1 gene (ABCA1), apolipoprotein B gene (APOB), apolipoprotein E gene (APOE) and lipoprotein lipase gene (LPL) single nucleotide polymorphism site genotypes to assess the genetic risk of individual susceptibility to abnormal cholesterol metabolism. Background technique [0002] Cholesterol is the most abundant sterol compound in the body. It is not only a component of cell biofilm, but also a precursor of steroid hormones, bile acids and vitamin D. Therefore, for most tissues, it is very important to ensure the supply of cholesterol to maintain their metabolic balance. Cholesterol widely exists in various tissues of the body, of which about 1 / 4 is distrib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 冯哲民邹祖烨
Owner HAINAN ZHUJIAN BIOTECH
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