Microelectrode array chip for cell electrofusion

Abstract

The invention provides a microelectrode array chip which is used to fuse cells electrically and is composed of a microelectrode array module, a fusion pool and a plate electrode module. The microelectrode array module is composed of a hard insulated basal layer and an electrode array layer, the electrode array layer introduces electrical signals through a metal wire, the fusion pool and the plate electrode module are composed of a base and the fusion pool on the base, the bottom of the fusion pool is also used as a plate electrode, the size of the electrode array layer on the microelectrode array module is smaller than the size of the fusion pool and the fusion pool on the plate electrode module, the microelectrode array module is covered on the fusion pool and the plate electrode module, the electrode array layer is dipped in sample solution of the fusion pool, and when external electric stimulation signals are implied, namely, an uneven gradient field with high strength is formed in tiny space between a microelectrode array and a plate electrode in the fusion pool, the process for electrically fusing the cells is realized. The chip can increase the efficiency for fusing the cells and the corrosion resistance of the chip, the biocompatibility of the microelectrode array chip is improved, and the safety and the cell vitality for fusing cells are guaranteed.

Description

technical field [0001] The invention relates to a chip structure for cell electric fusion. It specifically involves cell queuing in cell electrofusion experiments, cell electroporation, generation of electric field gradients and electric field strengths for cell electrofusion, sample injection and sample output for cell electrofusion experiments. Background technique [0002] Biological cells can form new cells through fusion, which is of great significance in the basic field of modern biomedical engineering. Cell fusion includes chemical fusion methods (such as PEG) and physical fusion methods. Among the physical fusion methods, the electric field fusion method has been widely used because of its advantages of easy precise control, good repeatability, and high fusion rate. [0003] When biological cells are in a non-uniform electric field, they are excited by the electric field to form dipoles, and the dipoles move under the force of the non-uniform electric field, that is...

AI Technical Summary

Problems solved by technology

Its advantage is that the number of integrated electrodes is large, and a large number of cells can be fused to a certain extent, but its disadvantage is that the flow channel is narrow, which is not conducive to the entry and exit of sample liquid and subsequent culture.

At the same time, the electric field strength and electric field gradient generated by the microelectrode are relatively weak, and the corrosion resistance and oxidation resistance of the processing material selection are also weak.

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