Content detecting method for lamioplomis rotate plaster
A determination method and a technology for plastering, applied in the field of detection, can solve the problems of inability to provide plaster, inability to provide and the like
Inactive Publication Date: 2008-09-10
SICHUAN BEILIKE BIOTECH LIABILITY
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Problems solved by technology
[0002] The main ingredient of the prescription of Duyiwei Babu Paste is the unique medicinal material. The 2005 edition of the Chinese Pharmacopoeia contains the unique medicinal material and the prescription preparation Duyiwei capsule. The unique capsule is an oral preparation, and the extraction process is water in advance. Yiwei babu ointment is pre-treated by water extraction and alcohol precipitation; this product is an external babu ointment, which is different from the oral preparation Duyiwei capsule in extraction process, preparation molding process, pharmacology, usage and dosage, etc., which cannot be obtained in the prior art. Provides a method that can accurately detect plasters containing the unique extract, so it cannot provide products with stable quality and consistent efficacy
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Embodiment 1
[0055] 1. Instruments and reagents
[0056] Instrument: Agilent 1100 high performance liquid chromatograph and supporting workstation;
[0057] Sartorius CP225D electronic balance;
[0058] Luteolin reference substance batch number: 111520-200504, for content determination, purchased from China Inspection Institute;
[0059] Reagents: Methanol for chromatography is chromatographically pure (Fisher Chemical Reagent Company, USA), water is double distilled water (self-made); other reagents are analytically pure.
[0060] 2. Chromatographic conditions
[0061] 2.1 The detection wavelength is 350nm.
[0062] 2.2 The mobile phase is methanol-0.4% phosphoric acid solution (50:50).
[0063] 2.3 Chromatographic column, with octadecylsilane bonded silica gel as filler.
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Abstract
The invention provides a content determination method of a lamiophlomis rotata emplastrum comprising High-Performance Liquid Chromatography (HPLC) adopted for determination; a reverse-phase chromatographic column adopted as chromatographic conditions; alkyd solution adopted as mobile phase, a UV detector adopted to determine the wavelength of 280 to 400nm, luteolin that is taken as the reference substance in which methanol is added to prepare the reference substance solution and the lamiophlomis rotata emplastrum added into 1 to 5mol / L methanol hydrochloride solution to prepare testing sample solution. After processes of reflux, extraction, taking out, cooling and filtering, subsequent filtrate is precisely measured, fetched and diluted by adding the methanol, thus obtaining the testing sample solution. The reference substance solution and the testing sample solution are precisely sucked and injected into a liquid chromatograph to finish the determination.
Description
technical field [0001] The invention provides a detection method, which is a method for determining the content of traditional Chinese medicine components, in particular, a method for determining the content of traditional Chinese medicine components using high-performance liquid phase. Background technique [0002] The main ingredient of the prescription of Duyiwei Babu Paste is the unique medicinal material. The 2005 edition of the Chinese Pharmacopoeia contains the unique medicinal material and the prescription preparation Duyiwei capsule. The unique capsule is an oral preparation, and the extraction process is water in advance. Yiwei babu ointment is pre-treated by water extraction and alcohol precipitation; this product is an external babu ointment, which is different from the oral preparation Duyiwei capsule in extraction process, preparation molding process, pharmacology, usage and dosage, etc., which cannot be obtained in the prior art. Provide a method that can accu...
Claims
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Patent Timeline
Login to View More IPC IPC(8): A61K36/53A61K9/70G01N30/02A61P17/02A61P7/04A61P15/00A61P1/02A61P19/08A61P29/00
Inventor 阙文彬廖立东熊正涛达晓玲
Owner SICHUAN BEILIKE BIOTECH LIABILITY