Method for preparing double-pore enzyme immobilization reactor
A technology of enzyme immobilization and reactor, applied to chemical instruments and methods, fixed on/in organic carriers, water/sludge/sewage treatment, etc., to achieve easy recycling, high fluid permeability, overcome The effect of poor loading parallelism
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Embodiment 1
[0016] Example 1: In a beaker, add 50mL of acetic acid aqueous solution with a pH of 5.0, then add 4.9g of polyethylene glycol, stir, add 24mL of tetramethoxysilane, stir at 0°C for 10min, add 0.1g of hexadecyl Trimethylammonium bromide, continue stirring at 0°C for 30min. The resulting mixture was poured into several polytetrafluoroethylene test tubes, placed in a 40°C water bath, and aged for 24 hours after reaching the gel point to obtain a wet silica gel column. Put the silica gel column after demoulding and rinsing in a constant temperature box, dry at 70°C for 12 hours, strictly control the water loss rate of the colloid, and then bake it in a box-type resistance furnace at 1°C min -1 The temperature was raised to 550° C. at a constant temperature for 4 hours to prepare a monolithic column of double-hole silica gel. The prepared double-hole monolithic column was placed in a polytetrafluoroethylene heat-shrinkable tube, and reacted at 180° C. for 4 hours to complete the ...
Embodiment 2
[0017] Example 2: In a beaker, add 50 mL of acetic acid aqueous solution with a pH of 5.0, then add 4.0 g of polyethylene glycol, stir, add 30 mL of tetramethoxysilane, and stir at 0° C. for 10 min. The resulting mixture was poured into several polytetrafluoroethylene test tubes, placed in a 40°C water bath, and aged for 24 hours after reaching the gel point to obtain a wet silica gel column. Soak the silica gel column after demoulding and rinsing in 0.01mol / L ammonia water, heat treatment at 120°C for 6 hours, then place it in a constant temperature box, and dry it at 70°C for 12 hours to strictly control the dehydration rate of the colloid, and then place it in the box-type resistor Roasting in the furnace at 1°C min -1 The temperature was raised to 550° C. at a constant temperature for 4 hours to prepare a monolithic column of double-hole silica gel. The prepared double-hole monolithic column was placed in a polytetrafluoroethylene heat-shrinkable tube, and reacted at 160°...
Embodiment 3
[0018] Example 3: In a beaker, add 50mL of acetic acid aqueous solution with a pH of 5.0, then add 5.2g of polyethylene glycol, stir, add 36mL of tetraethoxysilane, stir at 0°C for 10min, add 0.29g of citric acid, continue Stir at 0°C for 30 min, and adjust the pH of the solution to 5.0 with ammonia water. The resulting mixture was poured into several polytetrafluoroethylene test tubes, placed in a 40°C water bath, and aged for 24 hours after reaching the gel point to obtain a wet silica gel column. Put the silica gel column after demoulding and rinsing in a constant temperature box, dry at 70°C for 12 hours, strictly control the water loss rate of the colloid, and then bake it in a box-type resistance furnace at 1°C min -1 The temperature was raised to 550° C. at a constant temperature for 4 hours to prepare a monolithic column of double-hole silica gel. The prepared double-hole monolithic column was placed in a polytetrafluoroethylene heat-shrinkable tube, and reacted at 18...
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