Mixing mode expanded adsorbent bed medium and method for producing the same

Abstract

The invention discloses a mixed model expanded adsorbent bed medium and a preparation method thereof. In the composition of medium, matrix is a composite microsphere with fibrin / inorganic weighting agent, and petunidin is sulfonyl group and mercapto benzimidazole. The composite microsphere with fibrin / inorganic weighting agent is acquired by inverse suspension thermal regeneration and is taken asthe matrix to mix divinyl sulfone, soda buffer solution and DMSO for activation, then swab-off activation matrix, the mercapto benzimidazole and sodium hydroxide containing ammonium persulfate are mixed for coupling, thus acquiring the mixed model expanded adsorbent bed medium taking the mercapto benzimidazole and the sulfonyl group as the petunidin. The novel expanded bed adsorbing medium developed by the invention combines with a plurality of petunidin-protein interaction mechanisms such as hydrophobic interaction, charge induction action, thiophilic action, etc, to form mixed model adsorption, which has the advantages of large adsorption capacity, high selectivity, etc., thus being used for high efficient separation and purification of bioactivators such as antibody, etc.

Description

technical field The invention relates to a mixed-mode expanded bed adsorption medium and a preparation method thereof, which uses mercaptobenzimidazole and sulfone group as ligands, and belongs to the expanded bed adsorption and separation protein technology in chromatographic separation. Background technique Antibodies are an important biotechnology product widely used in immunotherapy, disease prevention, and medical diagnosis. In recent years, the separation technology for antibodies has made some progress, but it still needs to be continuously supplemented and improved. Conventional ion-exchange chromatography and hydrophobic interaction chromatography can bind antibodies, but their specificity and selectivity are poor, and there are many separation steps, which affect the activity yield of antibodies. Protein A affinity chromatography media can selectively adsorb antibodies, but the media is expensive, and the ligand is easily degraded by proteases in the feed solutio...

AI Technical Summary

Problems solved by technology

Therefore, the present invention uses cellulose / inorganic weighting agent composite microspheres as the expanded bed adsorption matrix, and the sulfone group introduced after divinyl sulfone activation and the coupled mercaptobenzimidazole are used as its main functional ligands to form a A new type of thiophilic mixed-mode expanded adsorption bed medium, which can be used for large-scale expanded bed adsorption and separation of antibodies, and has become a new type of efficient and integrated antibody separation process. Related research has not been reported in the literature