Magnetic immunoadsorption fiber and preparation method thereof

Abstract

The invention provides a magnetic immuno-adsorption fibre and a preparation method thereof. In the invention, a magnetic sol coating is coated on the fibre, the coating quantity of the magnetic matter on the fibre achieves 0.1-20 percent of the total weight; the magnetic sol is one of Fe, FeO, Fe3O4 and Fe2O3 or the composite thereof; wherein, the molar ratio of the Fe and the FeO is 0.01-10; themolar ratio of Fe and Fe3O4 is 0.02-1; the molar ratio of FeO and Fe3O4 is 0.05-2; the molar ratio of Fe3O4 and Fe2O3 IS 0.5-5; furthermore, the magnetic coating fibre is coupled by coupling agent soas to gain the magnetic immuno-adsorption fibre. By excellently combining the advantages of magnetic particles in immunoassay such as high-effect, fastness and no-dirt with high specific surface characteristic of the fibre, the adsorption efficiency is greatly improved and can be recovered and regenerated.

Description

technical field The invention belongs to the field of functional materials, in particular to an immunoadsorption column and a preparation method thereof. Background technique In vitro immunoadsorption therapy is an important part of blood purification therapy. Its principle is to firmly combine a single purified specific antibody (or antigen) on a fixed carrier to form an adsorption device (this device is called an immunoadsorption column). , and then connect it with the blood vessel, so that the blood passes through the device within a specified time, and fully contacts with the antibody (or antigen) on the adsorption column, and finally removes the harmful corresponding antigen (or antibody) in the blood completely. . In biological systems, there are many types of specific interactions, such as antigen-antibody, enzyme (receptor-substrate, complementary DNA strands, etc., immobilizing the complementary party (such as antigen) on the carrier, which can be Specifically ads...

AI Technical Summary

Problems solved by technology

Immunosorbent carrier substances include agarose gel, dextran, silica gel, resin, etc. These adsorbents have disadvantages such as slow flow rate, low adsorption efficiency, and low adsorption capacity.

At present, the immunosorbent based on silica gel and agarose gel can meet the needs of medical treatment, but it also has certain disadvantages: (1) the strength of silica gel is better, but the pH range of tolerance is narrow; (2) agar The hemocompatibility of sugar gel is good, but the strength is poor; (3) the shapes of both fillers are uniform bead particles, which can keep the column uniform during the filling process, but also make the column It is difficult to achieve a high flow rate in immunoadsorption therapy; (4) The packing of the bead particles is loose or too tight, and it is easy to form grooves or sedimentation during operation, which itself cannot act as a support, so the adsorption efficiency is low. Difficult to reuse

However, it is difficult to increase the adsorption capacity of the adsorption column made of membrane materials, and the practical application is of little significance

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