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Arthrinium bacterial strain and uses thereof

A technology of Arthrospora and bacterial strains, applied in the field of microorganisms, can solve the problems of incomplete molecular structure information and undetermined molecular configurations, etc.

Inactive Publication Date: 2008-10-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Cyclosporine is a class of tetrahydroxyanthraquinone compounds, first isolated in 1968, but due to incomplete molecular structure information, the exact molecular configuration of the molecule has not been determined

Method used

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  • Arthrinium bacterial strain and uses thereof
  • Arthrinium bacterial strain and uses thereof
  • Arthrinium bacterial strain and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, the isolation and cultivation of Arthrinium sp. bacterial strain:

[0021] In the laboratory, according to the following conditions and steps of isolation and cultivation, the Arthrophyllum strain of the present invention can be obtained:

[0022] 1), collect Polygonum Polygonum from Tianmu Mountain Nature Reserve in Zhejiang, my country, disinfect the collected Polygonum Polygonum with sodium hypochlorite at a concentration of 0.5% (w / v) for 10 minutes, then rinse with sterile water and place on sterile filter paper to absorb the water ;

[0023] 2) Next, use a sterile blade to remove the outer skin layer of Polygonum hydroponica, cut it into tissue pieces about 1 cm in size, and transfer the tissue pieces to each tissue containing 100 μg·ml -1 On 2% (w / v) water agar plate of ampicillin and streptomycin, culture at 24°C;

[0024] 3) After the hyphae grow out, the top of the mycelium is transplanted to the PDA solid medium for cultivation, and cultivated ...

Embodiment 2、2

[0025] Example 2. Separation, extraction and structural identification of 2,3β-dihydroxycritosporine:

[0026] 1), Arthrophyllum strain Ph1012 fermented culture, carry out following steps successively:

[0027] The Arthrospora strain Phl012 was activated and cultured on PDA medium at 26°C for 7 days, and the bacterial cake at the edge of the colony was cut with a puncher with a diameter of 5mm, and inoculated into a 500mL Erlenmeyer flask containing PDB culture medium, and each bottle was inoculated with 3 Bacteria cake, 26°C, 120r / min shaking culture for 15 days, the filtrate after removing the mycelium by suction filtration is the fermentation broth.

[0028] 2), extraction, recrystallization and structure identification:

[0029] Extract the fermentation liquid obtained in the above step 1) with ethyl acetate, and then recover the ethyl acetate under reduced pressure to obtain the extract; recrystallize the above extract with ethanol to obtain a red prismatic single crysta...

Embodiment 3、2

[0040] The toxicity determination of embodiment 3, 2,3β-dihydroxycridosporine:

[0041]Toxicity of 2,3β-dihydroxycitrasporine was determined by the toxicity test on Artemia salina. Artemia eggs were cultured and hatched at 28°C for 24 hours in artificial seawater with pH 8.5. Artificial seawater was mixed with a series of concentrations of ciprosporin solution in a ratio of 1:1 and added to the test tube, and each treatment was repeated three times. At the same time, distilled water and podophyllotoxin were used as blank control and positive control respectively. Add 30 normal Artemia in each test tube, observe and record the number of dead Artemia in each treatment after cultivating for 24hr, calculate the corrected mortality, adopt the dose logarithm-mortality probability value method to calculate and obtain the half-inhibitory concentration (LC 50 ). The details are shown in Table 4.

[0042] Table 4

[0043]

[0044] According to the content of the above-mentioned t...

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Abstract

The invention discloses an Arthrinium sp. strain which is preserved in China General Microbiological Culture Collection Center on June 8th, 2007 and has the preservation name of Arthrinium sp. strain Phl012 and the preservation number of CGMCC 2082. The Arthrinium sp. strain can be used for preparing 2,3beta-dihydroxy bostrycin which is a nontoxic red pigment with antibacterial activity.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to an endophytic fungus Arthrophyllum strain and its use in the preparation of 2,3β-dihydroxycritosporine. Background technique [0002] Discovering new lead compounds from natural products is an important way to create new drugs. Microorganisms are the main source of natural products in nature, and plant endophytic fungi, as an important group, are relatively new research fields. The abundant and diverse secondary metabolites produced by endophytic fungi have important application potential in modern medicine and pesticide industry. Aging and other fields have broad application prospects. A typical example is the discovery of taxol-producing endophytic fungi (Stierle et al., 1993). On the other hand, many new biologically active substances have also been found by relying on plant endophytic fungi, such as Rhinocladiella sp., an endophytic fungus isolated from the traditional medici...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/26C12R1/645
Inventor 章初龙陈绍瑗林福呈
Owner ZHEJIANG UNIV
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