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HLA-B27 magnetic ELISA reagent kit and method for producing the same

A technology of HLA-B27 and enzyme-linked immunosorbent reagent, which is applied in the field of medical devices to achieve the effect of good performance and low cost

Active Publication Date: 2012-11-14
GUANGZHOU PHICON BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

So far, no one has applied this technology to HLA-B27 antigen detection

Method used

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  • HLA-B27 magnetic ELISA reagent kit and method for producing the same

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Embodiment Construction

[0045] A HLA-B27 magnetic ELISA kit, including 1% BSA, 0.1% Proclin300 enzyme diluent in 0.01M PBS, anti-CD45 enzyme conjugate, coated with HLA-B27 antibody covalently coupled immunomagnetic Bead microplate strips, positive control for HRP-coupled HLA-B27 antibody molecules, substrate solution A, substrate solution B, stop solution, 20 times concentrated washing solution commonly used in ELISA kits, A negative control of 0.2MPBS, where, when used, the dilution of HLA-B27 antibody and CD45 enzyme conjugate was 1:50.

[0046] The preparation method of the above-mentioned HLA-B27 magnetic ELISA kit mainly includes the following steps:

[0047] (A) Covalent coupling of immunomagnetic beads and HLA-B27 antibody:

[0048] Wash 1ml immunomagnetic microbeads with PBS, pH7.0 for 3 times, add antibody 10mg / ml, 1ml in total, mix well, add bifunctional coupling agent BS 3 (Bis[sulfosuccinimidyl]suberate), make the final concentration 1mM, incubate at room temperature for 30min, add stop...

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Abstract

The invention discloses an HLA-B27 magnetic enzyme-linked immunoassay kit, essentially comprising enzyme diluent, enzyme combination and a micropore plate lath coated with immunomagnetic microbeads which are covalent and coupled with an HLA-B27 anti-body; the preparing method for the immunoassay kit comprises the following steps: (1) the immunomagnetic microbeads are covalent and coupled with theHLA-B27 anti-body; (2) the preparation of the enzyme combination; (3) the micropore plate is coated; the HLA-B27 magnetic enzyme-linked immunoassay kit has the advantages of rapidness, simpleness, correctness and low cost, etc.

Description

technical field [0001] The invention belongs to the field of medical devices, and in particular relates to an HLA-B27 magnetic ELISA kit and a preparation method thereof. Background technique [0002] Ankylosing spondylitis (AS) is a connective tissue disease with a prevalence rate of 0.2%-0.4% in the population, and the age of onset is mostly within 30 years old, accounting for 65%. The average course of disease is 4-6 years. The main symptoms are lumbar, sacral and lower limb joint pain. It is often accompanied by morning stiffness, heavy at night, and light after activity. Different from AS in adults, juvenile AS has fewer symptoms of bone lesions in the early stage of onset. It may be 24% of children complain of joint pain in limbs or only heel pain, but peripheral joint lesions are already obvious. AS mainly manifests as ossification of the intervertebral disc annulus fibrosus and connective tissue near the annulus fibrosus, inflammation and hyperplasia of the synovi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N33/535
Inventor 郭志程
Owner GUANGZHOU PHICON BIOTECH CO LTD
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