Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation of chicken interleukin-2 microsphere novel dosage form

A technology of interleukin and a new dosage form, which is applied in the field of biopharmaceutical preparation, can solve the problems of poor therapeutic effect and low utilization, and achieve the effects of convenient application, good degradation effect, and simple preparation process

Inactive Publication Date: 2008-11-05
TIANJIN SHENGJI GRP CO LTD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if these biological drugs are directly taken in water or taken orally, due to the degradation and digestion of enzymes in the gastrointestinal tract and the absorption barrier of the intestinal tract, their utilization is very low and the therapeutic effect is poor.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for preparing a new dosage form of chicken interleukin-2 microspheres, characterized in that the specific implementation steps are as follows:

[0023] (1) Add 50.0 mg chicken genetically engineered interleukin-2 to 8 ml of a solution with a pH of 4.5 to dissolve it (solution A);

[0024] (2) Take 40.0ml of polyethylene glycol-8-glycerol caprylic acid / capric acid ester, add solution A and stir to make 48ml. Stirring speed: 500 rpm; time: 3.0 hours; temperature: 20°C (solution B);

[0025] (3) Take 152ml of polyglycerol-3-oleate, add solution B and stir to form 200ml, stirring speed: 500 rpm, time: 3.0 hours, temperature: 20°C (solution C);

[0026] (4) Add 500 mg of trehalose and 500 mg of egg white protein to solution C; mix well and store in the refrigerator.

[0027] Using this method to make chicken interleukin-2 microspheres can increase the embedding rate of interleukin-2 to 82.6%, release 37.5% of the drug in artificial gastric juice within 2 hours, an...

Embodiment 2

[0029] A method for preparing a new dosage form of chicken interleukin-2 microspheres, characterized in that the specific implementation steps are as follows:

[0030] (1) Add chicken natural interleukin-220.0mg to 10ml of the solution with pH=3.5 and dissolve it (solution A);

[0031] (2) Take Tween 8014ml, decaglycerol monocaprylate 26ml, mix well, then add solution A to mix, stir well; stirring speed: 1000 rpm; time: 2.5 hours; temperature: 18°C ​​(solution B) ;

[0032] (3) Take 150ml of glycerol oleate, add the above B solution and mix it into 200ml; stirring speed: 1000 rpm; time: 2.5 hours; temperature: 18°C ​​(C solution);

[0033] (4) Add 100 mg trehalose and 200 mg bovine serum albumin to solution C, mix well, and store in the refrigerator.

[0034] Using this method to make chicken interleukin-2 microspheres can increase the embedding rate of interleukin-2 to 91.0%, release 36.2% of the drug in artificial gastric juice within 2 hours, and release 86.4% of the drug...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
embedding rateaaaaaaaaaa
embedding rateaaaaaaaaaa
Login to View More

Abstract

The invention relates to a preparation method of a new microsphere formulation chicken interleukin-2, which has the steps as follows: (1) the chicken interleukin-2 is dissolved in an aqueous phase buffer solution, the pH value of the solution (A) is 2.5 to 7.0 and the content of the chicken interleukin-2 is 1.0 to 10.0 mg / ml; (2) the acid solution (A) containing the chicken interleukin-2 is added into a liquid nonionic type surfactant solution, the hydrophile-lyophile balance value of which is HLB being more than or equal to 9 and less than or equal to 15, or into amphipathic grease, or into the intermixture of the nonionic type surfactant solution and the amphipathic grease to be mixed as a solution (B); (3) the solution (B) is added into liquid oil phase with the hydrophile-lyophile balance value of HLB being more than or equal to 0 and less than or equal to 9, or into a lipophilic emulsifier, or into the intermixture of the oil phase and the lipophilic emulsifier. The ratio of the solution (B) and the intermixture ranges from 1:1 to 1:9 and the two solutions are stirred and mixed at the temperature of 5 DEG C to 30 DEG C to form an oil phase formulation (C); (4) a stabilizing agent is added. The embedment ratio of the interleukin-2 is improved to 60 to 92 percent by adopting the preparation method of the invention.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceutical preparation, and in particular relates to a preparation method of a new chicken interleukin-2 microsphere dosage form. Background technique [0002] At present, chicken interleukin-2, as an immunomodulator, has been widely used clinically. Chicken Interleukin-2 is a natural immune enhancer. It has the functions of activating T cells, promoting and maintaining long-term survival of T cells; promoting B cell differentiation, producing antibodies, and inducing interferon production. Recombinant chicken interleukin-2 is used in chickens and has a good immune enhancing effect on vaccines. It can overcome the shortcomings of high toxicity and difficult absorption caused by other conventional adjuvants, and can also overcome the immunosuppressive state caused by certain diseases, and reduce the major economic losses caused by the immunosuppression caused by pathogenic infection in the poultry...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/20A61K9/16A61K47/42A61K47/34A61K47/36A61P31/12A61P37/04A61K47/14A61K47/26
Inventor 古长庆
Owner TIANJIN SHENGJI GRP CO LTD