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Seaweed ferment probiotic microcapsule and preparation method thereof

A seaweed enzyme and microcapsule technology, applied to bacteria, lactobacilli, bifidobacteria, etc. used in food preparation, can solve the problems of affecting the physiological efficacy of probiotics, difficult to control and store, and the reduction of viable bacteria, and achieve good results. Effects of bioadhesive properties, increased loss, and rapid cyst formation

Pending Publication Date: 2020-08-28
QINGDAO BRIGHT MOON SEAWEED BIO HEALTH TECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently used probiotic products have the characteristics of being difficult to control and store. After entering the human digestive tract, they will be affected by unfavorable factors such as low pH gastric acid, digestive enzymes, and bile acids, resulting in the number of live bacteria in the human intestinal tract. Significantly reduced, affecting the beneficial physiological effects of probiotics
Taking such probiotic products for the human body cannot really enhance the resistance and effectively fight against harmful bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0034] The preparation method of seaweed enzyme probiotic microcapsule of the present invention comprises:

[0035] (1) Preparation of activated probiotic strains: Inoculate one or more probiotic powders into the sterilized and cooled MRS liquid medium, and after the constant temperature culture at 37°C enters the stable period, centrifuge at 4000r / min and wash with normal saline Wash and collect concentrated bacterial liquid; MRS liquid medium is: beef extract 10.0g, peptone 10.0g, yeast extract 5.0g, sodium acetate trihydrate 5.0g, glucose 20.0g, diammonium citrate 2.0g, MgSO 4 7H 2 O 0.58g, K 2 HPO 4 2.0 g, MnSO 4 ·H 2 O 0.2g, Tween 801.0mL, distilled water 1000mL, pH6.5, sterilized at 121°C for 20min.

[0036] (2) Preparation of seaweed enzyme liquid: chop and soak fresh seaweed raw materials for 30-50 minutes, add compound enzymes to enzymolyze at 35°C, pasteurize, inoculate at 30-50°C and ferment for 3-6 days, then obtain by solid-liquid separation Seaweed enzyme s...

Embodiment 1

[0041] (1) Preparation of activated probiotic strains: operate under sterile conditions, inoculate Lactobacillus reuteri powder into the sterilized and cooled MRS liquid medium, seal the container mouth, and culture at a constant temperature of 37°C After 20 hours, the bacterial cells entered the stable phase, centrifuged at 4000 r / min for 10 min, washed twice with 0.9g / 100mL sterile saline, and concentrated bacterial liquid was collected.

[0042] (2) Preparation of seaweed enzyme solution: chop fresh wakame and soak in water for 30 minutes, add compound enzymes and enzymolyze at 35°C for 2 hours, pasteurize and inoculate food-grade Bacillus subtilis and Lactobacillus plantarum for fermentation fermented in tanks, fermented at 40°C for 4 days, and obtained liquid wakame enzyme through solid-liquid separation.

[0043] (3) Prepare the core material mixture: Mix 20 mL of the concentrated Lactobacillus reuteri liquid in step (1) and liquid wakame enzyme in step (2) and mix evenl...

Embodiment 2

[0050] (1) Activation of probiotic strains: operate under aseptic conditions, inoculate the bacterial powder of Lactobacillus plantarum and Bifidobacterium lactis into the sterilized and cooled MRS liquid medium, seal the container mouth, and keep the temperature at 37°C After the culture was allowed to stand for 18 hours and the bacteria entered the stable phase, they were placed in a centrifuge at 4000 r / min for 10 minutes, washed 3 times with 0.9g / 100mL sterile saline, and the concentrated bacterial solution was collected.

[0051] (2) Preparation of seaweed enzyme solution: chop fresh Enteromorpha and soak in water for 30 minutes, add compound enzymes and enzymolyze at 35°C for 2 hours, pasteurize and inoculate food-grade Bacillus subtilis in a fermenter at 45°C After fermenting for 4 days, liquid Enteromorpha ferment was obtained by solid-liquid separation.

[0052] (3) Prepare the core material mixture: mix 30mL of the concentrated bacterial solution of Lactobacillus pla...

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Abstract

The invention provides a seaweed ferment probiotic microcapsule and a preparation method thereof. The microcapsule comprises a microcapsule wall, a microcapsule core material and a core material carrier, wherein the microcapsule wall is prepared from sodium alginate, chitosan and calcium chloride, the microcapsule core material is prepared from seaweed ferment and probiotic, and the core materialcarrier is corn starch. The seaweed ferment probiotic microcapsule of the invention can effectively block the influence of gastric acid and the like on the viable bacteria number and activity of the probiotic, so that the seaweed ferment and probiotic act on the intestinal micro-ecological environment together, and the unique biological effect is achieved. The preparation method is simple in process, is relatively low in cost, and is easy to store and transport. Compared with other probiotic products, the microcapsule has the advantages of good film-forming property, strong stability, high probiotic survival rate, good slow release efficiency and the like, is suitable for embedding and controllable release of various probiotics, and has good application prospects.

Description

technical field [0001] The invention relates to a seaweed enzyme probiotic microcapsule and a preparation method thereof, belonging to the technical field of biological health care products. Background technique [0002] Probiotics refer to the active beneficial bacteria living in the human gastrointestinal tract, and they form the first line of defense against diseases in the body. Probiotic preparations refer to microecological preparations that add a certain amount of beneficial bacteria to humans, animals and plants, and have beneficial health effects on the host. At present, there are more than 20 kinds of probiotics used in China, mainly Bifidobacterium, Lactobacillus, Clostridium butyricum, Enterococcus and so on. Clinical and experimental studies have shown that probiotic preparations can synthesize niacin, folic acid and other vitamins, promote protein digestion and absorption, affect the composition and function of intestinal flora, promote intestinal epithelial c...

Claims

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Application Information

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IPC IPC(8): A23L33/135A23L33/00A23L29/00A23L29/256A23L29/30A23L3/40A23L3/46A23P10/30
CPCA23L33/135A23L33/00A23L29/015A23L29/256A23L29/30A23L3/40A23L3/46A23P10/30A23V2002/00A23V2400/147A23V2400/11A23V2400/125A23V2400/123A23V2400/173A23V2400/113A23V2400/175A23V2400/321A23V2400/169A23V2400/513A23V2400/529A23V2400/517A23V2400/249A23V2400/51A23V2200/3204A23V2250/1578A23V2250/511A23V2300/10
Inventor 邱霞张国防姜进举王璐璐张健李可昌王发合
Owner QINGDAO BRIGHT MOON SEAWEED BIO HEALTH TECH GRP CO LTD
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