Purification enrichment method of Guangdong purple beautyberry total flavone part

A technology of total flavonoids and purple beads, applied in the field of pharmacognosy, can solve the problems of lack of purification and enrichment methods of total flavonoids, achieve good impurity removal effect, efficient method, and improve adsorption effect

Active Publication Date: 2011-08-24
JIANGSU KANION PHARMA CO LTD +1
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the current problem of the lack of a good method for purifying and enriching the total flavonoids of the Chinese medicinal material Guangdong Zizhu, the present invention is proposed

Method used

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  • Purification enrichment method of Guangdong purple beautyberry total flavone part

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: The influence of acidity on adsorption rate

[0017] Sample preparation

[0018] Precisely weigh 10g of the dried Guangdong Purple Pearl powder (passed through a 24-mesh sieve), place it in a 500ml round-bottomed flask, and extract twice with distilled water, each with 150ml of the corresponding solvent, the first extraction is 2 hours, the second Extract for 1.5 hours each time, filter with suction after each extraction, combine the filtrate and the lotion of the lotion residue, and appropriately concentrate them in a 50ml volumetric flask to obtain the Guangdong Zizhu extract.

[0019] The method for the determination of total flavonoids in the samples refers to the determination of the total flavonoids of Sophora japonica in the 2005 edition of the People’s Republic of China Pharmacopoeia.

[0020] In this embodiment, the pH values ​​are respectively 2.0, 3.0, 4.0, and 5.0 for investigation. Measure 5ml each of the processed weak polar or non-polar macroporous ad...

Embodiment 2

[0023] Example 2 The influence of the amount of water used in washing and removing impurities on purity and yield

[0024] Measure 15ml of the processed non-polar or weakly polar macroporous adsorption resin, load it into a column, load 40ml of column solution (total flavonoids 7.8mg / ml), load the column at a flow rate of 1ml / min, repeat the adsorption once, and let stand for 24h Then, 0.5, 1, 2, 3, and 4 times the volume of the resin were used to rinse and remove impurities, and then eluted with 100 ml of 70% ethanol. The total flavonoid concentration of each part was measured separately, and the total flavonoid content and yield were calculated.

[0025]

[0026] Note: According to the existing technical content, if it is necessary to wash to colorless as mentioned in the references, it needs more than 4 times the resin volume to wash. From the data provided in this example, it can be seen that when the washing amount reaches 2-3 times the resin volume At that time, the impuritie...

Embodiment 3

[0027] Example 3 Comparison of adsorption rate between static adsorption and dynamic adsorption

[0028] Measure two portions of the processed weakly polar or non-polar macroporous adsorption resin, 5ml each, and add 25ml of Guangdong purple bead extract (total flavonoids 7.8mg / ml). One sample solution is statically adsorbed for more than 2 hours Method; another sample solution adopts dynamic adsorption method. After the adsorption is complete, the concentration of the unadsorbed sample solution is measured and the adsorption rate is calculated.

[0029] Adsorption method

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Abstract

The invention provides a purifying and enriching method of parts of total flavonoids of callicarpa kwangtungensis chun. The invention is designed against that the Chinese herbal medicine callicarpa kwangtungensis chun is lack of the purifying and enriching method of the parts of the total flavonoids with the good effects. The purifying and enriching method of the invention changes the absorption performances of the parts of the total flavonoids of the callicarpa kwangtungensis chun by regulating pH value of extract liquid of the callicarpa kwangtungensis chun and adopts the static absorption mode, and the method can effectively improve the enrichment efficiency of the parts of the total flavonoids of the callicarpa kwangtungensis chun. In addition, the method only uses small amount of water after the absorption on the column of the extract liquid to achieve the effect of removing impurities, thus being the purifying method which saves resources and time.

Description

Technical field [0001] The invention relates to the field of pharmacognosy, in particular to a preparation process of Guangdong Zizhu, a Chinese medicinal material. Background technique [0002] Guangdong Zizhu is mainly used for the treatment of gynecological inflammatory diseases. For example, Kanggongyan Tablets, Kanggongyan Granules, Gongfukang Capsules, etc. are all compound preparations with Guangdong Zizhu as the main medicine, which has performed well in clinical practice. According to statistics, cervicitis accounts for about 40-50% of the total number of obstetrics and gynecology clinics, ranking first among gynecological diseases. Therefore, it is of universal significance to strengthen the in-depth development of Guangdong Zizhu. Guangdong purple bead is the dry stems, branches and leaves of Callicarpa kwangtungensis Chun. Mainly produced in Pingxiang, Anyi, Yichun, Anyuan, Jinggangshan, Anfu and other places. Synonyms: Hemostatic Chai, Zizhucao, Zizhu, Jindaocai, O...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/85A61P15/00A61K135/00A61K127/00
Inventor 杨义芳王艳晶
Owner JIANGSU KANION PHARMA CO LTD
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