Purification enrichment method of Guangdong purple beautyberry total flavone part

A technology of total flavonoids and purple beads, which is applied in the field of pharmacology, can solve the problems of lack of a method for purification and enrichment of total flavonoids, and achieves the effects of good impurity removal effect, time saving, simple method and the like

Active Publication Date: 2008-11-26
JIANGSU KANION PHARMA CO LTD +1
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0005] Aiming at the current problem of the lack of a good method for purifying and enriching the total flavonoids of the Chinese medicinal material Guangdong Zizhu, the present invention is proposed

Method used

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  • Purification enrichment method of Guangdong purple beautyberry total flavone part

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1; The influence of acidity on adsorption rate

[0017] sample preparation

[0018] Accurately weigh 10g of dried Guangdong purple pearl powder (through a 24-mesh sieve), place it in a 500ml round bottom flask, extract twice with distilled water, add 150ml of corresponding solvent each time, extract for the first time for 2 hours, and extract for the second time Extract for 1.5 hours each time, suction filter after each extraction, combine the filtrate and the lotion of the washing dregs, and properly concentrate in a 50ml volumetric flask to obtain the Guangdong purple pearl extract.

[0019] The determination method of the total flavonoids in the sample refers to the determination of the total flavonoid content of Sophora japonica in the 2005 edition of the Pharmacopoeia of the People's Republic of China.

[0020] In this embodiment, the pH values ​​are respectively 2.0, 3.0, 4.0 and 5.0 for investigation. Measure 5ml each of the treated weakly polar or n...

Embodiment 2

[0023] The impact of the amount of water used for embodiment 2 washing and impurity removal on purity and yield

[0024] Measure 15ml of the treated non-polar or weakly polar macroporous adsorption resin, pack it into a column, put 40ml of the column solution (total flavonoids 7.8mg / ml), put it on the column at a flow rate of 1ml / min, repeat the adsorption once, and let it stand for 24h Finally, wash and remove impurities with water of 0.5, 1, 2, 3, 4 times the volume of the resin, and then elute with 100ml of 70% ethanol, measure the concentration of total flavonoids in each part respectively, and calculate the content and yield of total flavonoids.

[0025]

[0026] Explanation: According to the content of the prior art, if water washing to colorless as mentioned in the references is required, water washing of more than 4 times the volume of the resin is required. From the data provided in this example, it can be seen that when the amount of water washing reaches 2-3 times...

Embodiment 3

[0027] Embodiment 3 compares the adsorption rate of static adsorption and dynamic adsorption

[0028] Measure two parts of weakly polar or non-polar macroporous adsorption resin, each 5ml, add 25ml of Guangdong purple pearl extract (total flavonoids 7.8mg / ml) precisely, and use static adsorption for more than 2 hours for one part of the sample solution method; the other sample solution adopts the method of dynamic adsorption. After the adsorption was complete, the concentration of the unadsorbed sample solution was measured respectively, and the adsorption rate was calculated.

[0029] Adsorption method

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Abstract

The invention provides a purifying and enriching method of parts of total flavonoids of callicarpa kwangtungensis chun. The invention is designed against that the Chinese herbal medicine callicarpa kwangtungensis chun is lack of the purifying and enriching method of the parts of the total flavonoids with the good effects. The purifying and enriching method of the invention changes the absorption performances of the parts of the total flavonoids of the callicarpa kwangtungensis chun by regulating pH value of extract liquid of the callicarpa kwangtungensis chun and adopts the static absorption mode, and the method can effectively improve the enrichment efficiency of the parts of the total flavonoids of the callicarpa kwangtungensis chun. In addition, the method only uses small amount of water after the absorption on the column of the extract liquid to achieve the effect of removing impurities, thus being the purifying method which saves resources and time.

Description

technical field [0001] The invention relates to the field of pharmacognosy, in particular to a preparation process of Guangdong Zizhu, a traditional Chinese medicinal material. Background technique [0002] Guangdong Zizhu is mainly used in the treatment of gynecological inflammatory diseases, such as Kanggongyan Tablets, Kanggongyan Granules, Gongfukang Capsules, etc., which are compound preparations with Guangdong Zizhu as the main drug, and have played a very good role in clinical practice. Efficacy, according to statistics, cervicitis accounts for about 40-50% of the total number of obstetrics and gynecology outpatients, ranking first in gynecological diseases. Therefore, it is of universal significance to strengthen the in-depth development of Guangdong Zizhu. Guangdong Zizhu is the dry stem, branch and leaf of the Verbenaceae plant Guangdong Zizhu (Callicarpa kwangtungensis Chun.). It is mainly produced in Pingxiang, Anyi, Yichun, Anyuan, Jinggangshan, Anfu and other ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/85A61P15/00A61K127/00A61K135/00
Inventor 杨义芳王艳晶
Owner JIANGSU KANION PHARMA CO LTD
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