Degradable pH-sensitive hydrogel copolymer, method for preparing same and use
A technology of copolymer and hydrogel, applied in the field of P copolymer and its preparation, can solve the problems of non-biodegradation and clinical application limitation, and achieve the effects of controllable properties, simple operation and high purity
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Embodiment 1
[0039] The synthesis of embodiment one present invention P (CL-MAA-EG) polymkeric substance
[0040] 1. Synthesis of HEMA-PCL macromer:
[0041] 2-Hydroxyethyl methacrylate-terminated PCL macromonomer (HEMA-PCL) was initiated by caprolactone (ε-caprolactone, ε -CL) (0.3mol) was synthesized by ring-opening polymerization, in which tin octoate (Tin(II)2-ethylhexanoate, Sn(Oct)2) was used as a catalyst. Then, the HEMA-PCL macromonomer was purified by dissolution / precipitation method, and then the macromolecular monomer was vacuum-dried at 25° C. to constant weight. The purified material was stored in the refrigerator until use.
[0042] 2. Synthesis of P(CL-MAA-EG) hydrogel:
[0043] Photopolymerization provides an efficient and gentle method for in situ hydrogelation. Mild photopolymerization has many advantages over traditional physicochemical hydrogel formation methods: such as neutral reaction conditions, minimal by-products, no need for potentially toxic catalysts and st...
Embodiment 2
[0058] The preparation of embodiment two P (CL-MAA-MPEG) hydrogel
[0059] The method is basically the same as in Example 1, except that one end is a double bond, and the other end is methoxy-terminated as PEG, that is, MPEG-MA is used instead of PEGDMA, and P(CL -MAA-MPEG) hydrogel, because only one end of MPEG contains double bonds in the hydrogel, the pH responsiveness of the resulting hydrogel is greatly enhanced compared with P(CL-MAA-EG) hydrogel.
[0060] Implementation of the preparation of three P (CL-MAA-EG) hydrogel drug-loading system of the present invention
[0061] Swell the P(CL-MAA-EG) hydrogel of the present invention in pH7.2 first, then add water-soluble drugs such as VB 12 Or BSA, etc. At this time, the water-soluble drug will diffuse into the hydrogel. After 1-2 hours, add a buffer solution of pH 1.2 to the system to lower the pH value of the system and shrink the hydrogel, and the drug will be easily absorbed. Loaded into the interior of the hydrogel, ...
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