Surgery biological sticking patch and preparation method thereof

A biological patch and surgical technology, applied in the field of biomedical engineering, can solve the problems of air leakage at the pinhole, anastomotic tear, unsuitable for lung wound treatment, etc., to accelerate the formation of granulation tissue, prevent the formation of scar tissue, and maintain normal effect on lung function

Inactive Publication Date: 2008-12-03
上海契斯特医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Suture reinforcement seems to be the most economical and convenient option, but this method increases the operation time and is not suitable for the treatment of large lung wounds
Because the suturing will lead to normal lung tissue atelectasis, and the suture surface is highly tense, there are often negative reports of air leakage at the needle hole during the lung filling period, and often tearing of the anastomotic port, resulting in re-bleeding and air leakage.
At present, all clinical and animal experimental research results on bioglue have failed to provide convincing evidence to prove that it has the effect of strengthening the sutured tissue strength and reducing the incidence of bleeding and air leakage
Moreover, the bioglue tends to dissolve too fast, has weak strength, has poor adhesion to lung tissue after coagulation, is easy to fall off, and is expensive and cumbersome to use.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of acellular matrix from porcine small intestinal mucosa

[0022] Step 1: Take the fresh small intestine tissue of a one-year-old pig, carefully cut off the attached fat tissue, cut open the intestinal lumen, and place it in phosphate-buffered saline (PBS) for transportation and storage.

[0023] Step 2: Put the obtained peritoneum into hypotonic tris buffer solution (hypotonic tris buffer, Ph 7.5) containing protease inhibitor phenylmethyl-sulfonyl fluoride (phenylmethyl-sulfonyl fluoride, 0.20-0.5ml / L) -8.5), 0-20 ° C shaker mixing treatment for 24-48 hours.

[0024] Step 3: add 1-5% mild detergent Triton to the above solution, and continue to treat for 24-48 hours. Flush thoroughly with Hank's simulated body fluid.

[0025] Step 4: Add DNase and treat with RANase at 36-38°C for 1 hour

[0026] Step 5: Repeat the triton treatment for 24-48 hours and then soak in Hank's solution for two to five days.

[0027] The prepared above-mentioned product is used...

Embodiment 2

[0028] Embodiment 2 (be the preferred scheme of embodiment 1)

[0029] Preparation of acellular matrix from porcine small intestinal mucosa

[0030] Step 1: Take the fresh small intestine tissue of a one-year-old pig, carefully cut off the attached fat tissue, cut open the intestinal lumen, and place it in phosphate-buffered saline (PBS) for transportation and storage.

[0031] Step 2: Put the obtained peritoneum into a hypotonic tris buffer solution (hypotonic tris buffer, Ph 8.0) containing protease inhibitor phenylmethyl-sulfonyl fluoride (0.35ml / L) , 4 ° C shaker mixing treatment for 24 hours.

[0032] Step 3: add 1% mild detergent Triton to the above solution, and continue to treat for 24 hours. Flush thoroughly with Hank's simulated body fluid.

[0033] Step 4: Add DNase and treat with RANase at 37°C for 1 hour

[0034] Step 5: Repeat triton treatment for 24 hours and then soak in Hank's solution for two days.

[0035] The prepared above-mentioned product is used as...

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PUM

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Abstract

The invention belongs to the biomedical engineering field, in particular relating to a surgical biological patch and a preparation method thereof. The surgical biological patch takes a decellation technology as the basis and a biological source epithelial tissue material as a raw material, is mainly applicable to the stanching and the air leakage prevention of a lung wound surface after a thoracic surgery operation, and can be used for the stanching of wound surfaces of various parenchymal viscera (such as livers, spleens and so on).

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and in particular relates to a surgical biological patch and a preparation method thereof. Background technique [0002] Hemorrhage and air leakage from wounds of various solid organs are common complications during surgery. Taking thoracic surgery as an example, after various types of lung surgery, the bleeding and air leakage of the wound surface of the lung tissue prolong the operation time and increase the mortality rate of the operation. Small amounts of bleeding and air leaks from stitch holes can heal on their own. Severe postoperative bleeding, exceeding 200ml per hour, requires emergency thoracotomy to stop bleeding. Severe postoperative air leaks (greater than 7 days, approximately 15% of all patients) suggest bronchopleural leaks, often leading to empyema, requiring prolonged or replaced chest tubes, intrapleural chemical injections causing pleural adhesions, and even re-opening...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
Inventor 谭强潘银根金跃辉殷青
Owner 上海契斯特医疗科技有限公司
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