Application of celery native ester stearic acid in glutamic acid fermentation

A fatty acid ester and glutamic acid technology, applied in the field of biochemistry, can solve problems such as no reports of glutamic acid fermentation

Inactive Publication Date: 2008-12-10
GUANGZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Apigenin stearate is one of the surfactants. It is a non-ionic surfactant with good emulsification, foaming and softness. It is rarely reported in the application. It is mainly used in In the oil industry, it is used as an antioxidant and has achieved good results, but there is no report on its use in glutamic acid fermentation

Method used

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  • Application of celery native ester stearic acid in glutamic acid fermentation
  • Application of celery native ester stearic acid in glutamic acid fermentation
  • Application of celery native ester stearic acid in glutamic acid fermentation

Examples

Experimental program
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Effect test

example 1

[0062] Strain: Corynebacterium glutamicum T-613, preserved by Guangzhou Institute of Microbiology.

[0063] The fermentation process consists of two steps of strain cultivation and fermentation:

[0064] (1) Strain culture: connect a ring of bacteria from the slope to the seed medium, fill 20mL of liquid in a 250mL shaker bottle, rotate at a speed of 96r / min, temperature 33°C, culture time 12-14h, 0D 650 Values ​​greater than 0.5 culture termination.

[0065] (2) Fermentation: Insert the seed culture solution into the fermentation medium according to 5% inoculation amount, 250mL liquid volume is 20mL, the shaker speed is 96r / min in the early stage of fermentation 0-16h, the temperature is 33.5°C, and the speed in the middle stage of fermentation is 115r / min in 16-40h min, temperature 36°C, 40-48h late stage of fermentation, shaker speed 105r / min, temperature 38°C, add 0.05‰ apigenin stearate at 12h, culture time 48h;

[0066] in,

[0067] Described seed culture medium (%) c...

example 2

[0071] Strain: Corynebacterium glutamicum T-613, preserved by Guangzhou Institute of Microbiology.

[0072] The fermentation process consists of two steps of strain cultivation and fermentation:

[0073] (1) Strain cultivation: connect a ring of bacteria from the slope to the seed medium, fill 20mL of liquid in a 250mL shaker bottle, rotate at a speed of 96r / min, temperature 33°C, culture time 12-14h, OD 650 Values ​​greater than 0.5 culture termination.

[0074] (2) Fermentation: Insert the seed culture solution into the fermentation medium according to the inoculum size of 5%, 250mL liquid volume is 20mL, the shaker speed is 96r / min in the early stage of fermentation 0-16h, the temperature is 33.5°C, and the speed in the middle stage of fermentation is 115r / min in 16-40h min, temperature 36°C, 40-48h late stage of fermentation, shaker speed 105r / min, temperature 38°C, add 0.2‰ apigenin stearate at 16h, culture time 48h;

[0075] in,

[0076] Described seed medium (%) cons...

example 3

[0080] Strain: Corynebacterium glutamicum T-613, preserved by Guangzhou Institute of Microbiology.

[0081] The fermentation process consists of two steps of strain cultivation and fermentation:

[0082] (1) Strain cultivation: connect a ring of bacteria from the slope to the seed medium, fill 20mL of liquid in a 250mL shaker bottle, rotate at a speed of 96r / min, temperature 33°C, culture time 12-14h, OD 650 Values ​​greater than 0.5 culture termination.

[0083] (2) Fermentation: Insert the seed culture solution into the fermentation medium according to the inoculum size of 5%, 250mL liquid volume is 20mL, the shaker speed is 96r / min in the early stage of fermentation 0-16h, the temperature is 33.5°C, and the speed in the middle stage of fermentation is 115r / min in 16-40h Min, temperature 36°C, 40-48h late stage of fermentation, shaker speed 105r / min, temperature 38°C, add 0.1‰ apigenin stearate at 14h, culture time 48h;

[0084] in,

[0085] Described seed medium (%) consis...

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Abstract

The invention discloses a novel application for apigenin stearate, namely an application in the fermentation of glutamic acid. A small amount of the apigenin stearate is added at the middle and late stages of the fermentation of corynebacterium glutamicum, which can improve the output of the glutamic acid, almost has no influence on the survival and the growth of strains, and has better effect than addition of tween-60 and less adding amount. The invention also provides a method for improving the fermentation yield of the glutamic acid, which takes sugariness as a carbon source to produce the glutamic acid through the fermentation of the corynebacterium glutamicum. The method is characterized in that: the apigenin stearate which accounts for 0.05 to 0.2 thousandth of the total volume of zymotic fluid is added into the zymotic fluid when the fermentation is performed for 10 to 40 hours.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a method for promoting glutamic acid fermentation. Background technique [0002] Glutamic acid is obtained through microbial fermentation, but glutamic acid is a metabolite in microbial cells, and its secreted products need to penetrate through the cell membrane to the outside of the cell. At the same time, the accumulation of glutamic acid in the cell has negative feedback on bacterial metabolism. The effect greatly affects the acid production rate of the microbial fermentation method. In glutamic acid fermentation, if the permeability of the cell membrane can be changed so that glutamic acid can continuously seep out of the cell, the production of glutamic acid will be accelerated. Studies have shown that one of the main factors affecting cell membrane permeability is the phospholipid content in the cell membrane. [0003] Breeding biotin-deficient strains is a common and effectiv...

Claims

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Application Information

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IPC IPC(8): C12P13/16C12R1/15
Inventor 郑成卫云路孙保兴宁正祥
Owner GUANGZHOU UNIVERSITY
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