Method for obtaining regeneration plant from in-vitro culture of black seed pumpkin

A technique for culturing and regenerating plants in vitro, applied in the field of plant tissue culture, can solve the problems of few reports, low regeneration frequency, sensitivity to root rot, etc., and achieve the effects of good repeatability of results, high regeneration frequency, and convenient material collection

Inactive Publication Date: 2008-12-17
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, black-seed squash also has many undesirable traits, such as susceptibility to root rot and resistance to root-knot nematode, etc., so genetic improvement research is needed
[0004] Compared with other melon crops (Colijn-Hooymansa, 1988; Trulsonand Shahin, 1986; Kathal, 1988; Moreno,

Method used

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  • Method for obtaining regeneration plant from in-vitro culture of black seed pumpkin
  • Method for obtaining regeneration plant from in-vitro culture of black seed pumpkin
  • Method for obtaining regeneration plant from in-vitro culture of black seed pumpkin

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Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1: In vitro culture of adventitious buds of black-seeded pumpkin germs induce plant regeneration:

[0024] 1. Materials and methods

[0025] 1.1 Plant material: Black-seeded pumpkin produced in Yunnan.

[0026] 1.2 Phytohormones and culture medium: The phytohormones used in this experiment were purchased from sigma company, namely thiadizuron (TDZ), kinetin (KT), and indole acetic acid (IAA). The basic medium is MS medium (Murashige T., Skoog F.A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant, 1962, 15: 473-497), during the culture process, according to different development stages in Different hormones are added in the culture medium, wherein, the induction medium of adventitious buds is MS+3% by weight of sucrose+0.9% by weight of agar+0.5mg / L thiadizuron; the elongation medium of adventitious buds is MS+ The agar+0.25mg / L kinetin of the sucrose+0.9% weight of 3% weight; The rooting medium is the agar+0.1mg / L in...

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Abstract

The invention discloses a method for obtaining regenerated plantlets by isolated culture of black-seeded pumpkin, comprising the steps of obtaining explant, inducing adventitious bud, adventitious bud extending, rooting cultivation and transplanting, wherein, the inducing culture medium of adventitious buds is MS, 3% sucrose by weight, 0.9% agaragar by weight and 0.5mg/L thidiazuron; the extending culture medium of adventitious buds is MS, 3% sucrose by weight, 0.9% agaragar by weight and 0.25mg/L kinetin; rooting culture medium is MS, 3% sucrose by weight, 0.9% agaragar by weight and 0.1mg/L indoleacetic acid. The invention overcomes the defects of low recurring frequency, long cultivation period, and the like, in the current recurring cultivation of black-seeded pumpkin plant, thereby establishing a technology of recurring system of high-frequency adventitious buds, providing an efficient practical technology for asexual propagation of black-seeded pumpkin and genetic transformation of black-seeded pumpkin induced by tumefaciens, and having certain significance.

Description

technical field [0001] The invention relates to a method for obtaining regenerated plants through in vitro culture of black-seeded pumpkin, which belongs to the field of plant tissue culture. Background technique [0002] Black seeded squash is an annual vine plant mainly distributed in high altitude areas, which is quite different from other species of the same genus, and it cannot hybridize with them to produce offspring. The center of origin has not yet been unified, and most people think it is the Americas. It is currently distributed mainly in Mexico, Chile, Germany, France, Japan, the Philippines, and China (Andrés 1990; Meng and Zeng 2000). Black-seeded squash is versatile, unripe fruit can be cooked and eaten or canned as a vegetable, and ripe fresh fruit can be used to make alcoholic beverages; the most important nutritional value of black-seeded squash is that its seeds contain a lot of protein and essential oils (Andrés, 1990); black-seeded squash also has the e...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 何启伟霍雨猛
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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