Method for obtaining regeneration plant from in-vitro culture of black seed pumpkin

Abstract

The invention discloses a method for obtaining regenerated plantlets by isolated culture of black-seeded pumpkin, comprising the steps of obtaining explant, inducing adventitious bud, adventitious bud extending, rooting cultivation and transplanting, wherein, the inducing culture medium of adventitious buds is MS, 3% sucrose by weight, 0.9% agaragar by weight and 0.5mg / L thidiazuron; the extending culture medium of adventitious buds is MS, 3% sucrose by weight, 0.9% agaragar by weight and 0.25mg / L kinetin; rooting culture medium is MS, 3% sucrose by weight, 0.9% agaragar by weight and 0.1mg / L indoleacetic acid. The invention overcomes the defects of low recurring frequency, long cultivation period, and the like, in the current recurring cultivation of black-seeded pumpkin plant, thereby establishing a technology of recurring system of high-frequency adventitious buds, providing an efficient practical technology for asexual propagation of black-seeded pumpkin and genetic transformation of black-seeded pumpkin induced by tumefaciens, and having certain significance.

Description

technical field [0001] The invention relates to a method for obtaining regenerated plants through in vitro culture of black-seeded pumpkin, which belongs to the field of plant tissue culture. Background technique [0002] Black seeded squash is an annual vine plant mainly distributed in high altitude areas, which is quite different from other species of the same genus, and it cannot hybridize with them to produce offspring. The center of origin has not yet been unified, and most people think it is the Americas. It is currently distributed mainly in Mexico, Chile, Germany, France, Japan, the Philippines, and China (Andrés 1990; Meng and Zeng 2000). Black-seeded squash is versatile, unripe fruit can be cooked and eaten or canned as a vegetable, and ripe fresh fruit can be used to make alcoholic beverages; the most important nutritional value of black-seeded squash is that its seeds contain a lot of protein and essential oils (Andrés, 1990); black-seeded squash also has the e...

AI Technical Summary

Problems solved by technology

However, black-seed squash also has many undesirable traits, such as susceptibility to root rot and resistance to root-knot nematode, etc., so genetic improvement research is needed

[0004] Compared with other melon crops (Colijn-Hooymansa, 1988; Trulsonand Shahin, 1986; Kathal, 1988; Moreno, 1985; Niedz, 1989), there are few reports on the regeneration system of black-seeded squash. The regeneration system is shoot tip expansion (Liu, 2004) and cotyledon node regeneration system (Xu, 2007), but there are disadvantages such as low regeneration frequency and long culture period.

Images