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Epidermal growth factor culture fluid, externally used drug for curing diabetes trauma using the culture fluid and preparation of the externally used drug

A technology of epidermal growth factor and culture medium, which is applied in the field of epidermal growth factor culture medium, external medicine for treating diabetic wounds and preparation thereof, can solve problems such as delayed treatment, and achieve the effects of promoting healing, safe use and simple use.

Inactive Publication Date: 2008-12-24
崔树玲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Occasional trauma among patients, often with disastrous consequences for delayed treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation method of the epidermal growth factor culture solution (hereinafter referred to as EGF) in vitro and the method of compounding with chitosan to prepare medicine.

[0024] 1. Main components:

[0025] 10ml each of epidermal growth factor culture solution and 3% chitosan suspension.

[0026] 2. Preparation method:

[0027] 1. In vitro culture and preparation technology of epidermal growth factor: 140-240 mg of living tissue of the mandibular gland of mammalian pups was extracted under sterile control conditions, quickly weighed, cut into pieces, washed 3 times by natural sedimentation in 1640 culture solution , placed in a culture bottle (50ml) containing 16ml of 1640 nutrient solution containing 20% ​​FCS, at 37°C, 5% CO 2 Cultured in the incubator for 6-7 days. During the cell culture process, the culture medium should be kept clear, all the supernatants were collected in stages, and stored in a freezer at -20°C.

[0028] 2. Preparation of Chitosan ...

Embodiment 2

[0035] The external medicine is made of the following raw materials in volume ratio: 20 ml of epidermal growth factor culture solution; 10 ml of 3% chitosan suspension.

Embodiment 3

[0037] The medicine for external use is made from raw materials with the following volume ratio: 10 ml of epidermal growth factor culture solution; 20 ml of 3% chitosan suspension.

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PUM

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Abstract

The invention discloses a medicine, in particular to an epidermal growth factor culture fluid, a drug for external use which treats wounds of diabetes by adopting the culture fluid, and a preparation method thereof. Polypeptide active factors and chitosan are obtained by a gland tissue of a young of mammal through the in vitro culture process. The invention optimizes and is compatible with the polypeptide active factors and the chitosan which stimulate cell growth in different action mechanisms in order to achieve the same cooperated and intensified effects for accelerating the wound cicatrization. The product is applied to the treatment of diabetes and various wounds, has the cicatrization accelerating effect superior to the conventional treatment, and achieves satisfactory healing effects on other ulcerous skin injury. The development of the product not only provides a novel effective way for the treatment of the ulcer of diabetes wounds but also provides a better, rapider and more effective way for the conventional treatment of the wounds.

Description

(1) Technical field [0001] The invention relates to a medicine, in particular to an epidermal growth factor culture solution, an external medicine for treating diabetic wounds with the culture solution and a preparation method thereof. (2) Background technology [0002] Diabetes is one of the "three major killers" that endangers human health and ranks at the forefront of various diseases. constitute a huge patient population all over the world. According to statistics in recent years, the annual incidence rate in China is above 3.2%, and it is on the rise, and the incidence situation is becoming increasingly severe. Gangrene and amputation caused by diabetic trauma are one of the serious consequences of diabetic complications, and the treatment of diabetic trauma is still a thorny problem that plagues the medical field. Incidental trauma among patients often has disastrous consequences for delayed treatment. The whole world is looking for efficient preparations for the tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06A61K35/55A61K9/70A61P17/02A61P3/10A61K38/18A61K31/722C12N5/071
Inventor 崔树玲钟华钟峻
Owner 崔树玲
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