Black bamboo tissue culture medium and tissue culture and rapid propagation method

A technology of tissue culture medium and tissue culture rapid propagation, applied in horticultural methods, botany equipment and methods, cultivation, etc.

Inactive Publication Date: 2009-01-07
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After retrieval and market investigation, there has been no material report and related objects published so far that the explant tissue of mature purple bamboo is used to induce regeneration plants

Method used

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Examples

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Embodiment Construction

[0013] The present invention is described in further detail below in conjunction with the examples: my country's bamboo tissue culture regenerated plants is still in its infancy, and scattered bamboo is more difficult to succeed than clustered bamboo because of its weak organ regeneration ability. What has been achieved is to reproduce buds without dedifferentiation, and use young plants, embryos and other tissues. There is no report of using adult plant explants to regenerate plants. Practitioners generally agree that it is difficult to root the tissue cultured seedlings of scattered bamboo species. Therefore, the main attack direction of the present invention is to create an excellent artificial environment, starting from improving the tissue cell proliferation and rooting conditions of the purple bamboo, to overcome the difficulties in the tissue culture of the scattered bamboo. For this reason, a series of comparative experiments have been done on the selection and matchin...

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Abstract

The invention relates to a tissue culture medium for the purple bamboo, which is composed of a clustered bud induction culture medium and a root induction culture medium. The clustered bud induction culture medium comprises the following components: MS, 3.0 mg / L of BA, 0.01 mg / L of TDZ, 0.25% of the total weight of crystal agar and 3% of the total weight of sucrose. The root induction culture medium comprises the following components: MS, 3mg / L of BA, 1mg / L of NAA, 0.25% of the total weight of crystal agar and 3% of the total weight of sucrose. Both the pH values of the two culture media are 5.0. The method of tissue culture and rapid propagation using the culture media comprises the following four steps: collection and sterilization of explants, induction of clustered buds, multiplication and rapid propagation of adventitious buds, and root induction and transplanting. With the tissue culture and rapid propagation method using the culture media, the clustered bud induction rate reaches 42%, and the adventitious bud multiplication coefficient is as high as 13 times, the root induction rate reaches 90%, and the survival rate of transplanting test-tube plantlets is about 90%. The invention overcomes the difficulty in tissue culture and plant regeneration of the running bamboo and provides a new approach to the rapid propagation and cultivation of the purple bamboo for yard planting, landscaping and large-area afforestation.

Description

【Technical field】 [0001] The invention relates to a tissue culture medium and a tissue culture rapid propagation method of scattered bamboos with medium and small diameters, in particular to a tissue culture medium and a tissue culture rapid propagation method of purple bamboo. 【Background technique】 [0002] Purple bamboo (Phyllostachys nigra), also known as black bamboo and black bamboo, belongs to the genus Bambusa in the Poaceae Bamboo subfamily. It is a scattered bamboo species. The new stems are green, and the biennial stems gradually turn purple-black. The green leaves are green and graceful, and they are highly ornamental. It is also an excellent material for making furniture, musical instruments and handicrafts. It has been propagated by transplanting mother bamboo or bamboo whip, which is slow, requires a lot of land, inconvenient transportation, high cost and restricted by seasons. In recent years, there are more and more people who are interested in exploring bam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00C12N5/04
CPCY02A40/22Y02P60/40
Inventor 林新春方伟桂仁意杨海芸王晓芹黄丽春
Owner ZHEJIANG FORESTRY UNIVERSITY
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