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Method for preparing immobilization cold resistant strain

A cold-resistant, bacterial strain technology, applied in the direction of biochemical equipment and methods, chemical instruments and methods, and microbial-based methods, can solve the problems of slow growth and low removal rate of organic pollutants

Inactive Publication Date: 2009-01-07
HARBIN INST OF TECH
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  • Summary
  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation method for immobilized psychrophilic bacteria in order to solve the problem of slow growth of bacteria isolated in the prior art at low temperature and low removal rate of organic pollutants in treated sewage

Method used

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  • Method for preparing immobilization cold resistant strain
  • Method for preparing immobilization cold resistant strain

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specific Embodiment approach 1

[0009] Specific embodiment one: the preparation method of immobilized psychrotrophic bacteria in this embodiment is as follows: 1, the cultivation and domestication of bacterial classification: put the sludge in the aeration tank of winter sewage treatment system into the low-temperature biochemical culture that temperature is 15 ℃ In the tank, the mixed sewage composed of domestic sewage and simulated sewage at a volume ratio of 1:1-3 is introduced, and then the sludge concentration is 2.0-3.0g / L, and the dissolved oxygen is 2-4mg / L. Expose the sludge for 8-10 hours, then settle for 2-4 hours, then change the water once a day and then discharge 1 / 3 of the supernatant, and discharge all the supernatant after 2-4 days of operation, and then put it in the water with a hydraulic retention time of 6 -8h, under the condition of 2-4h static settling time, lower the temperature of the low-temperature biochemical incubator to 10°C for 8-10 days, then lower it to 8°C for 8-10 days, and ...

specific Embodiment approach 2

[0011]Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that 6 bacterial strains were screened out in step 2. After testing, these 6 bacterial strains belonged to the genus Kineticum, Aeromonas, Flavobacterium, and Microbacteria, respectively. Bacteria, Bacillus and Pseudomonas. Others are the same as in the first embodiment.

[0012] The immobilized psychrotrophic bacteria obtained in this embodiment were tested in a composite bioreactor, and the sewage was treated in the composite bioreactor successively through two treatment processes: activated sludge treatment and biological contact oxidation treatment, wherein activated sludge treatment During the process, the sludge concentration is 2.0-3.0g / L, the dissolved oxygen concentration is 2.0-4.0mg / L, and the immobilized psychrophilic bacteria obtained in this embodiment are added to the composite In the bioreactor, the dosage of immobilized psychrophilic bacteria is 25-35% of the effe...

specific Embodiment approach 3

[0015] Embodiment 3: This embodiment differs from Embodiment 1 in that the sludge concentration in step 1 is 2.1-2.9 g / L. Others are the same as in the first embodiment.

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Abstract

A preparation method for an immobilized psychrophile relates to a preparation method for an immobilized psychrophile. The invention solves the problems of slow growing during low temperature and low removing rate for the organic contaminations in the processed sewage of the thalli separated by the prior art. The method of the invention includes as follows: step one, the culturing and domestication of the strain; step two, the filtering of psychrophile; step three, the fixing of the immobilized psychrophile. The removing rate of the immobilized psychrophile of the invention to the COD under the environment of low temperature is higher by 63.67 percent than the bacteria with middle temperature; simultaneously the removing rate of the immobilized psychrophile of the invention to the COD under the environment of middle temperature is higher by 6.02 percent than that of the immobilized psychrophile under the environment of low temperature. The average removing rate of the immobilized psychrophile of the invention to the COD of the sewage in the winter is 85.79 percent; the average value of the removing rate for the COD is 86.66 percent. The immobilized psychrophile of the invention does not lose the activity as the temperature is lower in the winter.

Description

technical field [0001] The invention relates to a preparation method of psychrotrophs. Background technique [0002] The long winter and low temperature in northern my country lead to a decrease in the activity of microorganisms. Therefore, the effect of biological treatment of sewage in winter is very poor. This is mainly due to the adsorption performance, sedimentation performance, microbial growth and development, and population composition of activated sludge due to the reduction in temperature. And the oxygen transfer efficiency in the aeration tank has a significant impact. [0003] At present, it has been reported that cold-resistant bacteria with biodegradability to petroleum hydrocarbons, chlorophenols, aromatic hydrocarbons, fats, surfactants and other organic substances, sulfur-containing nitrogen-containing gases, and sulfates in sewage have been isolated from low-temperature environments. However, compared with medium temperature conditions, this kind of cold-re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/04C12N11/10C12N11/04C02F3/34C02F11/02C12R1/01
CPCY02W10/20
Inventor 贲岳陈忠林徐贞贞姜安玺
Owner HARBIN INST OF TECH
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