Method for preparing high colour value red rice pigment using rice as raw material

A technology of red yeast pigment and high color value, applied in the field of producing red yeast pigment, can solve the problems of low color value, high labor intensity, high degree of automation, etc.

Inactive Publication Date: 2009-01-07
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are two main methods of producing red yeast pigment: one is the solid-state fermentation extraction method, in which Monascus is inoculated into cooked rice, fermented by solid-state culture, dried and ground, and then extracted with alcohol It is obtained by extracting the pigment from it with organic solvents and drying it. The biggest advantage of the solid-state fermentation method for preparing red yeast pigment is that it requires less investment, but the biggest disadvantage is that it is labor-intensive. And add water, inoculate Monascus strains through liquid fermentation, then filter, concentrate and dry. Its advantage is high degree of automation, and its disadvantage is that the one-time investment is relatively large
And the existing preparation method, no matter it is the solid-state fermentation extraction method, or the liquid state fermentation extraction method, the red yeast pigment prepared by it, the color value is all lower

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Weigh 1 kilogram of rice, break it into 80 mesh fineness, add 12 times of drinking tap water, soak it in a fermenter for 10 minutes, add amylase equivalent to 0.2% of the weight of rice and keep it warm for 70 minutes at 45°C to obtain Rice milk; (2) adding sodium nitrate (NaNO3) equivalent to 0.2% rice milk weight in rice milk 3 ), 0.25% potassium dihydrogen phosphate (KH 2 PO 4 ), 0.25% magnesium sulfate heptahydrate (MgSO 4 ·7H 2 (2), 1% corn steep liquor, 2% soya bean powder, adjust pH with lactic acid to be 5.5, and airtight fermenter is heated to 121 ℃ and keeps 20 minutes, is cooled to 40 ℃, makes fermentation medium; (3) Monascus strain expands Cultivation: After cultivating and cultivating Monascus strains on a solid slant, inoculate them into the seed medium and cultivate them at 31°C for 50 hours to obtain the expanded culture medium of Monascus strains for production; Add 10% of the weight of the culture medium to the fermentation medium cooled to 40...

Embodiment 2

[0020] (1) Weigh 2 kg of rice, break it into 80 mesh fineness, add 12 times of drinking tap water, soak it in a fermenter for 8 minutes, add 0.15% amylase equivalent to the weight of rice, and keep it warm at 38°C for 80 minutes to obtain rice (2) in rice milk, add sodium nitrate, 0.2% potassium dihydrogen phosphate, 0.2% magnesium sulfate heptahydrate, 1.5% corn steep liquor, 2% soybean powder that are equivalent to 0.25% of rice milk weight, and adjust the pH with lactic acid to 5.0, the airtight fermenter was heated to 121°C for 20 minutes, cooled to 38°C, and the fermentation medium was obtained; (3) Expanded cultivation of Monascus strains: the Monascus strains were cultured on a solid slope and inoculated into the seeds Substratum was cultivated at 31°C for 50 hours to obtain the expanded culture solution of Monascus strains for production; (4) Fermentation culture: adding the expanded culture solution of Monascus strains equivalent to 8% of the weight of the fermentation...

Embodiment 3

[0023] (1) Weigh 1.5 kg of rice, break it into 90 mesh fineness, add 12 times of drinking tap water, soak it in a fermenter for 15 minutes, add 0.15% amylase equivalent to the weight of rice, and keep it warm for 90 minutes at 40°C to obtain rice (2) add 0.12% sodium nitrate (NaNO 3 ), 0.22% potassium dihydrogen phosphate (KH 2 PO 4 ), 0.22% magnesium sulfate heptahydrate (MgSO 4 ·7H 2 (2), 1.2% corn steep liquor, 2.2% soy flour, adjust the pH to 5.0 with lactic acid, heat the closed fermenter to 121°C and keep for 20 minutes, and cool to 38°C to obtain the fermentation broth; (3) Monascus strain expansion Cultivation: After cultivating and cultivating Monascus strains on a solid slant, inoculate them into the seed medium and cultivate them at 31°C for 50 hours to obtain the expanded culture medium of Monascus strains for production; Add 9% of the weight of the culture medium to the fermented culture medium cooled to 36°C, and incubate and stir it for 100 hours at 32°C. Th...

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PUM

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Abstract

A method for manufacturing monascus pigment with a high color value by taking rice as a material includes the following steps: (1) preparing rice pulp; (2) adding 0.2 to 0.25 percent of sodium nitrate, 0.15 to 0.25 percent of monopotassium phosphate, 0.15 to 0.25 percent of heptahydrate magnesium sulfate, 1 to 1.5 percent of corn pulp and 1.5 to 2 percent of bean powder calculated by the weight of the rice pulp into the rice pulp, heating to 121 DEG C and maintaining for 20 minutes and cooling to 35 DEG C to 40 DEG C below zero to prepare a fermentation culture medium; (3) after the monascus pigment is cultured by a solid incline, inoculating the monascus pigment into a seed culture medium and culturing for 50 hours to prepare the enlarging culture medium of a monascus strain ; (4) adding the enlarging nutrient liquid, the quantity of which is equal to 8 to 10 percent of the weight of the monascus strain, into the fermentation culture medium, stirring and culturing for 85 to 100 hours and maintaining the pressure of a fermentation pot to be 0.04 to 0.06MPa to prepare a fermentation liquid; (5) filtering the fermentation liquid, obtaining a condensed liquid after membrane condensing is carried out on a filter liquid, then drying in a spraying or freezing way, namely obtaining the monascus pigment. Detected by a detecting method regulated by the national standard of GB15961-2005, the color value of the monascus pigment prepared by the invention can achieve more than 200.

Description

technical field [0001] The invention relates to a preparation method of high-color-valence monascus pigment, in particular to a method for preparing monascus pigment by using rice as a raw material through microbial liquid fermentation technology. Background technique [0002] Red yeast rice is a traditional Chinese medicinal and edible rice fermented product, which has a history of more than 1,000 years in my country. It is made by solid-state culture and fermentation in rice with the unique Chinese monascus fungus, and it is dried and ground. Because it contains red pigment, it is dark red, so it is called red yeast rice. It used to be a health food for ancient emperors, and there was a record of "cooking meat with red yeast rice" in the Sui and Tang Dynasties. More than 600 years ago, Li Shizhen, an ancient Chinese pharmacist, included red yeast rice in the "Compendium of Materia Medica" for the first time, and accurately explained the medicinal functions of red yeast ri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C12R1/66
Inventor 林亲录
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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