Biology artificial hepatocyte reactor

Abstract

The invention provides a novel biological artificial liver cell reactor. The novel biological artificial liver cell reactor is technically characterized in that a cover is arranged at the upper end of an outer shell; the exit of culture fluid is arranged at the lower end of the outer shell; two layers and more than two layers of flat plates are arranged in gravity center symmetry; the middle of each layer of the flat plates is provided with round holes, the surface is provided with more than two small holes, and the surface of the flat plate is spinned with a layer of chitosan nanometer fiberscaffold; the flat plates are fixed on the hollow scaffold by the central round holes; the side face of the hollow scaffold is provided with side holes. The novel biological artificial liver cell reactor solves the limitations of the prior art existing in the hollow fiber type, the single layer flat plate type, the infusion bed type and the wrapped suspension type biological artificial liver cellreactors, and increases the effective exchange area of the reactor, realizes the full exchange of culture substances due to the adherent culture of the liver cells and the uniform distribution of thecells, improves the adherence of the liver cells on the cell scaffold surface, and therefore bears the shear stress brought by the culture liquid circulation.

Description

technical field [0001] The invention relates to a medical device, in particular to a novel bioartificial liver cell reactor. [0002] Background technique [0003] Bioartificial liver can provide short-term liver function support for patients, and it is an effective adjuvant therapy for diseases such as liver failure and has a good application prospect. As the core component of the bioartificial liver, the bioreactor determines the activity and function of the cells in it, and thus determines the performance of the bioartificial liver. [0004] Prior to the present invention, currently clinically used bioreactors were roughly divided into four types: hollow fiber type, single-layer flat plate type, perfusion bed type and coated suspension type. Among them, the hollow fiber bioreactor is the most widely used at present. It seals a bundle of hollow fiber tubes in the shell of transparent organic materials. Hepatocytes were cultured. The fiber also has the function of a semi...

AI Technical Summary

Problems solved by technology

[0004]However, the reactor has certain defects in use

Firstly, the fibers in the reactor are densely arranged, difficult to expand, and the capacity and effective exchange area are small; secondly, the cells are accumulated and cultured outside the hollow fibers, which tend to settle at the bottom and cannot be evenly distributed among the fibers; thirdly, the cells are densely packed In culture, the exchange of nutrients and oxygen is limited, which limits the function and reduces the activity of the cells; finally, the hepatocytes are anchor-like cells, and the accumulation and growth will also cause a decrease in the activity of the cells

In the perfusion bed system, although the hepatocytes can adhere to the wall, they tend to fall off easily because they cannot withstand the shear force brought by the liquid circulation.

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