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Method for preparing microbial oil by irradiation of microorganism with 60Co gamma-ray

A microbial oil, gamma ray technology, applied in the production of fat, fat oil/fat production, fermentation, etc., to achieve the effect of high positive rate of change, simple process operation, and lower production costs

Inactive Publication Date: 2009-01-28
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, no use 60 Production of Microbial Oil by Coγray Irradiated Microorganisms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0019] (1) Strains: Rhodotorula gutini As2.107, purchased from the Institute of Microbiology, Chinese Academy of Sciences.

[0020] (2) Medium:

[0021] ① Solid slant medium (complete medium)

[0022] Yeast powder: 10g / L; peptone: 20g / L; glucose: 20g / L; agar: 20g / L; pH=5.

[0023] ②Seed medium

[0024] Glucose: 15g / L; (NtH 4 ) 2 SO 4 : 2g / L; yeast powder: 1g / L; KH 2 PO 4 : 7g / L; Na 2 HPO 4 : 2g / L; MgSO 4 : 1.5g / L; pH=5.5.

[0025] ③ Fermentation medium

[0026] Glucose: 50g / L; KNO 3 : 0.38g / L; yeast powder: 4g / L; KH 2 PO 4 : 6g / L; Na 2 HPO 4 : 2g / L; MgSO 4 : 2g / L; CaCl 2 : 0.1g / L; FeCl 3 : 0.07g / L; pH=5.5; sterilized at 115°C for 20min.

[0027] (3) Preparation of yeast suspension

[0028] Transfer the preserved Rhodotorula viscosus to the slant medium for activation, wash the bacteria with 10ml sterile water as much as possible, and count the cells under a microscope to adjust the cell concentration to 7×10 6 individual / mL.

[0029] (4) 60 Co gamma ray...

example 2

[0036] (1) Bacteria: the same as Example 1.

[0037] (2) Medium:

[0038] ①Solid slant medium (complete medium): Same as Example 1.

[0039] ②Seed culture medium: same as Example 1.

[0040] ③Fermentation medium: same as Example 1.

[0041] ④ Complete medium (g / L): yeast extract powder 3.0, beef extract 10.0, tryptone 10.0, soluble starch 1.0, glucose 5.0, L-cysteine ​​0.50, NaCI 5.0, NaAc 3.0, agar powder 15, pH8. 5.

[0042] ⑤ Complete medium for regeneration

[0043] Add 0.5mol / L sucrose (or 0.8mol / L mannitol or 1mol / L sorbitol) to the complete medium.

[0044] ⑥ Double layer regeneration complete medium

[0045] The upper layer needs to add 0.6% agar to the complete medium; the bottom layer is the complete medium.

[0046] (3) Preparation of protoplasts

[0047] Activate the preserved starting strain, insert it into the liquid medium, shake and culture for 16 hours, centrifuge, discard the supernatant, wash with hypertonic buffer, add 0.2% β-mercaptoethanol and 0.5...

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PUM

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Abstract

The invention provides a method for preparing PUFAs by using <60>Co Gamma ray for irradiating microorganism, which belongs to the technical field of biochemical engineering. The method adopts the <60>Co Gamma ray to irradiate oil-yielding microorganism mycelium or protoplast thereof so as to obtain high-yielding strain for oil production to prepare the PUFAs. The method has the advantages that the technological operation is simple, the irradiated mycelium and protoplast have high forward-mutation rate, and the method can obviously enhance the oil productivity of the oil-yielding microorganism, thus reducing the production cost of the PUFAs.

Description

technical field [0001] The invention belongs to the field of biochemical technology, in particular to a 60 A method for preparing microbial oil by irradiating microorganisms with Co gamma rays. Background technique [0002] Biodiesel is a renewable and clean energy developed in recent years based on environmental degradation and energy crisis that can replace fossil fuels. At present, the research on biodiesel preparation at home and abroad mainly focuses on vegetable oil or waste catering oil, but the cost of using vegetable oil as raw material is high, and its cost accounts for 70% to 85% of the total production cost. The difficulty of collecting and transporting raw materials has seriously restricted the industrialization process of biodiesel, so finding a cheap raw material has become the key to the industrialization of biodiesel. [0003] Microbial oil, also known as single cell oil, is made of microorganisms such as yeast, mold, bacteria, and algae under certain cond...

Claims

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Application Information

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IPC IPC(8): C11B1/00C12P7/64
Inventor 刘宏娟张建安武敏敏周玉杰程可可
Owner TSINGHUA UNIV
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