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Method for determining protein, sodium, kalium, calcium, magnesium in prescription nutrition food

A nutritious food and protein technology, applied in the field of sodium, determination of protein, calcium, potassium and magnesium in infant formula food, can solve the problems of complicated operation, large amount of acid, matrix interference, etc.

Inactive Publication Date: 2009-01-28
北京市大兴区疾病预防控制中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method for determining the protein content in formula nutritional food for infants and young children still uses the Kjeldahl method, and the operation is complicated; while the metal elements of sodium, potassium, calcium and magnesium are generally determined by atomic absorption spectrophotometry. Different amounts of anti-interference reagents need to be replaced with hollow cathode lamps of corresponding elements; the traditional determination methods of the above indicators still have problems such as long digestion time, large amount of acid, low efficiency of the determination method, serious matrix interference, poor reproducibility, etc. question

Method used

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  • Method for determining protein, sodium, kalium, calcium, magnesium in prescription nutrition food
  • Method for determining protein, sodium, kalium, calcium, magnesium in prescription nutrition food
  • Method for determining protein, sodium, kalium, calcium, magnesium in prescription nutrition food

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Effect test

Embodiment 1

[0039] Drawing of standard curve

[0040] Use the eluent to prepare a mixed standard curve according to Table 3, measure under the selected instrument conditions, set the standard curve drawing method of the instrument software to quadratic fitting, and automatically draw the instrument software with the concentration of each component and the corresponding peak area standard curve line. Standard curve see Figure 1-5 .

[0041] Table 3 Preparation method of mixed standard series of sodium, nitrogen, potassium, magnesium and calcium

[0042]

Embodiment 2

[0044] Separation Test of Sodium, Nitrogen, Potassium, Magnesium and Calcium on Ionpac CS12A Chromatographic Column

[0045] Prepare a single standard solution and a mixed standard solution with a concentration of 50 mg / L for sodium, nitrogen, potassium, magnesium, and calcium, inject and measure the sample under selected instrument conditions, and use the elution peak time of the single standard of each component for qualitative analysis. After the injection of the mixed standard solution, the eluted peak spectrum of the mixed standard solution is obtained, such as Figure 6 As shown, each adjacent component has a good separation effect. For the peak chromatograms of sodium, nitrogen, potassium, magnesium and calcium on the IonpacCS12A column Figure 6 . Table 4 is the data related to the component separation spectrum.

[0046] Table 4 Related data of component separation spectrum

[0047]

Embodiment 3

[0049] Sample digestion

[0050] Accurately weigh 3g of the mixed sample into a 250mL Kjeldahl flask, add 15mL of concentrated sulfuric acid and mix well with the sample, cover the small funnel and let it stand overnight. Put it on an electric furnace and heat it until it becomes black and viscous. During this process, if the content is black and lumpy, take it out and let it cool, mash it with a clean glass rod, wash the glass rod with 20mL boiling deionized water, and add the washing liquid into Kjeldahl Nitrogen flask; heat up to 300°C, cover with a small funnel and continue heating for 30 minutes. Take it out and let it cool, slowly add 20mL 30% hydrogen peroxide along the bottle wall, pay attention to wash down the carbon particles stained on the bottle wall, mix well, cover the small funnel and place it on the electric stove at 300°C to continue heating. If the solution is not transparent, continue to Repeat this step until the digestive solution in the bottle is colo...

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Abstract

The application provides a method that measures protein, sodium, potassium, calcium and magnesium in infant formula nutritional foods by utilizing an ion chromatograph, comprising the following steps: a sulphuric-acid-oxydol architecture is used for digesting a food sample, a zinc oxide neutralizing method or a hydrogen iodide reducing method is used for adjusting pH value, and the ion chromatograph is used for the measurement under specific conditions and the like. The measuring method can overcome the defects of low efficiency, severe matrix interference and bad reproducibility of measuring methods in the prior art.

Description

technical field [0001] This application relates to the field of food, and specifically relates to a method for determining protein, sodium, potassium, calcium and magnesium in formula food for infants and young children by using an ion chromatograph. Background technique [0002] Formula nutritional food for infants and young children mainly includes formula milk powder and formula cereal powder. Their high-quality products have played a role in promoting the normal growth and development of infants and young children. great harm. Therefore, it is of great significance to test the important nutritional indicators of this kind of products. At present, the method for determining the protein content in formula nutrition food for infants and young children still uses the Kjeldahl method, and the operation is complicated; while the metal elements of sodium, potassium, calcium and magnesium are generally determined by atomic absorption spectrophotometry. Different amounts of ant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/96
Inventor 房宁高洁
Owner 北京市大兴区疾病预防控制中心
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