Method for producing acetone-butanol using fermentation of corn bran raw material

A technology of acetone butanol and corn husks, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve the problems of reducing the utilization rate of raw materials, insufficient utilization of corn husk starch cellulose, etc. Effects of sustainable utilization, improved competitiveness, and improved utilization

Inactive Publication Date: 2009-02-11
上海天之冠可再生能源有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Corn bran is a by-product of corn starch produced by wet grinding of corn, in which the residual starch content is 20-30%, the protein content is about 10%, the cellulose content is about 18%, the hemicellulose is about 45%, and the lignin is about 1.0%. At present, corn husks are mainly used directly as feed for production. Although the cost of deep processing of corn husks has been reduced, the starch and cellulose in corn husks have not been fully utilized, which greatly reduces the utilization rate of raw materials.

Method used

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  • Method for producing acetone-butanol using fermentation of corn bran raw material
  • Method for producing acetone-butanol using fermentation of corn bran raw material
  • Method for producing acetone-butanol using fermentation of corn bran raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Direct cooking and fermentation of corn bran raw material ABE (bacteria are purchased from Institute of Plant Health, Chinese Academy of Sciences, the same below)

[0029] Weigh 37.5 grams of corn husks and add 150ml of water, adjust the pH to 7.0, a total of three groups of parallel samples, cook at 121°C for 1 hour, cool to about 37-40°C, and ferment with ABE strains, the fermentation temperature is 37°C, and the fermentation cycle is 48 hours .

[0030] After the fermentation was finished, the fermented liquid was centrifuged at 12,000 rpm for 5 minutes, and the contents of acetone, butanol and ethanol were determined by gas chromatography after taking out. The solvent determination results are shown in Table 1.

[0031] Table 1 ABE results of corn bran raw material fermentation

[0032]

[0033] It can be seen from the results in the table that when the residual starch in the corn husk is relatively high, the residual starch can be used for ABE fer...

Embodiment 2

[0034] Embodiment 2: mixed fermentation of corn husk and cassava

[0035] According to the solid-liquid ratio of 1:5, 1:7, 1:9, weigh the corn husks and mix them with water, add 3%, 4%, and 5% cassava flour to the mixture of the three ratios, and each group has 3 parallel samples, 121 Cook at ℃ for 1 hour, cool to about 37-40℃, then ferment with ABE strain, the fermentation temperature is 37℃, and the fermentation cycle is 48 hours.

[0036] After the fermentation was finished, the fermented liquid was centrifuged at 12,000 rpm for 5 minutes, and the contents of acetone, butanol and ethanol were measured by gas chromatography after taking out. The solvent measurement results are shown in Table 2.

[0037] Table 2 Fermentation results of corn husk and cassava mixture

[0038]

[0039] It can be seen from the results in the table that when the residual starch content in the corn husk is low, some cassava flour can be added to it for fermentation, and the fermentation result ...

Embodiment 3

[0040] Embodiment 3: the mixed fermentation of corn husk and sweet potato powder

[0041] According to the solid-liquid ratio of 1:5, 1:7, 1:9, weigh the corn husks and mix them with water, add 3%, 4%, and 5% sweet potato flour to the mixture of the three ratios, and each group has 3 parallel samples, 121 Cook at ℃ for 1 hour, cool to about 37-40℃, then ferment with ABE strain, the fermentation temperature is 37℃, and the fermentation cycle is 48 hours.

[0042] After the fermentation was finished, the fermented liquid was centrifuged at 12,000 rpm for 5 minutes, and the contents of acetone, butanol and ethanol were measured by gas chromatography after taking out, and the results of the solvent determination were shown in Table 3.

[0043] Table 3 Mixed fermentation results of corn husk and sweet potato flour

[0044]

[0045] It can be seen from the results in the table that when the residual starch content in the corn husk is low, adding sweet potato powder to it for ferme...

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Abstract

The invention relates to a method for fermenting and producing acetone butanol by utilizing maize peel as raw material. The invention discloses a method for fermenting and producing acetone butanol by utilizing maize peel as raw material, which comprises fermentable product that is obtained by pretreatment of fermentation material and is inoculated with ABE strain for fermentation, and the acetone butanol product is obtained by filtering and distilling zymotic fluid; the method is characterized in that the fermentation material contains maize peel. The method uses residual starch and cellulose in the maize peel to ferment the ABE strain, meanwhile, residua also can be taken as feed again, so that a new way is provided for the production of ABE, and the problem of foodstuffs contention between the traditional ABE fermentation production and the people is solved, at the same time, the utilization rate of the maize peel material is improved, so that the method has important significance for improving the competitive strength of ABE fermentation industry and realizing the sustainable use of resources.

Description

technical field [0001] The invention relates to a method for producing acetone butanol by fermenting corn bran raw materials. Background technique [0002] Acetone, butanol and ethanol are important organic solvents and chemical raw materials, which are widely used in spray paint, explosives, plastics, pharmaceuticals, plant extraction, organic glass, synthetic rubber and other industries. Acetone can be used as a solvent for the manufacture of cellulose acetate films, plastics and coatings, and can also be used to produce chemical products such as methyl methacrylate (MMA), bisphenol A, and aldol condensates. Butanol is a new generation of biofuel. Compared with existing biofuels, butanol has a higher mixing ratio with gasoline, which does not require modification of vehicles. It also has significant environmental benefits and can reduce greenhouse gas emissions. Therefore, Butanol will occupy an important proportion in the future transportation fuel structure. Ethanol is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/36C12P7/16C12P7/10A23K1/14C12R1/01A23K10/12A23K10/37
CPCY02E50/16Y02E50/10Y02P60/87
Inventor 沈兆兵杜风光史吉平杨晟刘钺顾阳杨蕴刘姜卫红陈波董义伟孙沛勇
Owner 上海天之冠可再生能源有限公司
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