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Immobilisation of bacteria to a geological material

A technology of bacteria and geological bodies, applied to soil conditioning materials, chemical instruments and methods, fixed on or in inorganic carriers, etc., can solve the problems of adding mineralization media without teaching, flocculation solidification of bacteria, etc., to achieve Achieves mineral deposition, enhanced effectiveness effects

Inactive Publication Date: 2009-02-11
三角洲研究基金会
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It does not teach immobilization of bacteria in the composition by flocculation, nor does it teach the application of an additional step of adding mineralizing media after bacterial immobilization

Method used

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  • Immobilisation of bacteria to a geological material
  • Immobilisation of bacteria to a geological material
  • Immobilisation of bacteria to a geological material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Flocculation of bacteria

[0047] Salts of different divalent cations were mixed with a suspension of bacteria of the genus Sporosarcina pasteurii (optical density at 600 nm = 1.0) at a temperature of 20°C to generate a final concentration of 20 mM divalent cations . These salts are Ni(NO 3 ) 2 , ZnCl 2 , Mg(NO 3 ) 2 , CaCl 2 .

[0048] After 5 minutes of mixing, the optical density at 600 nm was determined and compared to a control (bacterial suspension without added divalent cations). figure 1 Results are shown.

[0049] From the increase in optical density, it can be deduced that nickel, zinc, magnesium, and calcium are effective in initiating flocculation.

Embodiment 2

[0050] Embodiment 2: Determination of leakage point capacity

[0051] Use Itterbeek grit (d 50 = 420 μm) A column (circular ID (circular ID) of 1.6 cm, 1 m long) was packed to 1.7 g / cm 3 Density.

[0052] Inject 10 ml of 50 mM calcium chloride in a pulse. Thereafter, the column pumps calcium chloride through the column filled with ionized water and monitors the chloride level at the end of the column. image 3 It is shown that calcium chloride migrates through the material without being substantially delayed: after about one column volume, the front of the calcium chloride band reaches the end of the column, and at Within 1.5 column volumes, substantially all of the calcium chloride migrated through the material.

[0053] In a separate experiment, 10 ml of a suspension of Sporosarcina pasteurii bacteria (optical density at 600 nm 4.2) were injected in a pulse. Tests showed that the front of the band including bacteria reached the end of the column after more than one co...

Embodiment 3

[0054] Example 3: Immobilization of bacteria

[0055] With Itterbeek sand (d 50 = 420 μm) to fill a column (1.8 cm circular diameter, 6.8 m long) and keep it under saturation. Bacteria were injected (concentration = 0.5 mS / min) until they emerged at the column outlet. Immediately thereafter, the same volume of 50 mM calcium chloride was injected and allowed to stand for 1 hour. The column was then rinsed with three pore volumes of deionized water and fractionated for urease activity analysis.

[0056] Urease activity was determined by weighing a known volume of sand into a container and adding urea to a final concentration of 1M, monitoring the conductivity to obtain the urea hydrolysis rate (mS / min).

[0057] Urease activity was detected at various positions along the 6.8 m length of the column ( image 3 ).

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Abstract

The invention relates to a method for immobilising bacteria in a material permeable to the bacteria, in particular a geological body, comprising forming a first zone comprising the bacteria in the material and allowing the first zone to move through at least part of the material; forming a second zone comprising an effective amount of a flocculating agent to the material and allowing the second zone to move through at least part of the material, wherein the zones are allowed to move such that the zones become at least partially overlapping and at least part of the bacteria flocculate, thereby becoming immobilised.

Description

technical field [0001] The present invention relates to methods for immobilizing bacteria in materials, especially geological bodies. Background technique [0002] Certain naturally occurring bacteria are known to induce biomineralization in natural environments. Based on this knowledge, Nemati and Voordauw in Enzyme and Microbial Technology 33 (2003), pp. 635-642 have described the modification of porous media with bacterial urease. [0003] It is contemplated that bacteria can be used without the need to isolate the urease from the bacteria. [0004] U.S. Patent 5,143,155 relates to a method of reducing the permeability of a subterranean geological formation by mineral subsidence, comprising providing the formation with a microbial culture capable of maintaining metabolic activity within the environment of the formation, supplying the formation with An aqueous mineralization medium is injected into the formation and mineralization is precipitated from the mineralization ...

Claims

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Application Information

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IPC IPC(8): C12N11/14C09K8/582
CPCC09K17/00C12N11/14C09K8/80
Inventor 莱昂·安德烈亚斯·范帕森维多利亚·苏珊·惠芬马里纳斯·彼得·哈尔克斯
Owner 三角洲研究基金会
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