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Method for synchronously extracting flavone and alkaloid from folium nelumbinis

A simultaneous extraction and lotus leaf flavonoids technology, which is applied in the direction of drug combinations, pharmaceutical formulas, plant raw materials, etc., can solve the problems of unremoved macromolecular impurities, low purity, low product yield, etc., and achieve easy operation and large-scale production , Improving the purity and quality, and the effect of simple process

Inactive Publication Date: 2009-02-18
WUHAN PUZETIAN FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing technical scheme for extraction of lotus leaf flavonoids and alkaloids has the disadvantage that the extract is not degreased and impurity-removed before being loaded on the column, and macromolecular impurities are not removed. Not high, difficult for large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Wash the fresh lotus leaves, air-dry them, crush them, and pass through 20 meshes to obtain lotus leaf powders; then mix the lotus leaf powders with 50% ethanol solution with a pH of 2, put them into an extraction kettle at a mass ratio of 1:15, and heat up to Extract twice at 60°C for 1 hour each time, and combine the extracts; concentrate the extracts under reduced pressure to 1 / 10 of the ethanol content to obtain a concentrated extract, and then filter to remove the precipitate; then add petroleum ether, stir and extract, and stand for 30 Minutes to remove fat-soluble impurities to obtain a raffinate; use 0.1mol / L NaOH solution to adjust the pH value of the raffinate to 8, let it stand, filter to remove alkali-insoluble impurities, and use 0.1mol / L acetic acid solution to adjust the pH value to neutral , add 10 times of distilled water to dilute the raffinate, filter it with 5040PP polypropylene ultrafiltration membrane at a temperature of 25°C and a pressure of 0.15M...

Embodiment 2

[0030]Wash the fresh lotus leaves, air-dry them, pulverize them, and pass through 20 meshes to obtain lotus leaf powders; then mix the lotus leaf powders with 70% ethanol solution with a pH of 3, put them into an extraction kettle at a mass ratio of 1:15, and heat up to Extract 3 times at 70°C for 2 hours each time, combine the extracts; concentrate the extracts under reduced pressure to 1 / 15 of the ethanol content to obtain a concentrated extract, then filter to remove the precipitate; then add petroleum ether, stir and extract, and stand for 1 hours, remove fat-soluble impurities, and obtain raffinate; use 0.1mol / L NaOH solution to adjust the pH value of the raffinate to 11, let it stand, filter to remove alkali-insoluble impurities, and adjust the pH value to neutral with 0.1mol / L acetic acid solution After adding 15 times of distilled water to dilute the raffinate, filter it with a 5040PP polypropylene ultrafiltration membrane at a temperature of 25°C and a pressure of 0.15...

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PUM

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Abstract

The invention relates to the deep processing field of farm and sideline products, in particular to a method for extracting flavone and alkaloid simultaneously from lotus leaves. The method is characterized in that the fresh lotus leaves are taken as raw materials and are subjected to pretreatment, acid alcoholic solution extraction, light petroleum derosination, hyperfiltration membrane concentration, purified alkaloid enrichment by gelatin highly acid positive ion exchange resin, adsorption and purification of flavone in lotus leaves by chromatography daiamid resin, and the like. Two products of the alkaloid and the flavone are obtained by one-step method. The invention has the advantages that the method is not only applied to the industrial production on large scale but also prepares the products with high purity as well as meets the requirement of the raw material extractive of national class 2 new drugs.

Description

technical field [0001] The invention relates to the field of deep processing of agricultural and sideline products, in particular to a method for synchronously extracting flavonoids and alkaloids from lotus leaves. Background technique [0002] The lotus leaf is a natural medicinal and edible ingredient. The lotus leaf is rich in bioactive factors such as flavonoids and alkaloids. Its "three reductions" and weight loss effects have attracted much attention in recent years. [0003] At present, there are many literature reports on the extraction of flavonoids and alkaloids in lotus leaves. Such as patent documents ZL02139048.7, CN 200610141952.0, CN 200610085626.2 all disclose the extraction method of lotus leaf flavonoids; although CN 200610069039.4, CN 200710009464.9 disclose the simultaneous extraction and separation of lotus leaf flavonoids and alkaloid method, but its shortcoming is that product impurity content is high, The purity is low, failing to meet the requiremen...

Claims

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Application Information

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IPC IPC(8): A61K36/62A61P3/04A61P3/06A61P3/10A61P3/12A61K127/00
Inventor 杨红鸽刘云
Owner WUHAN PUZETIAN FOOD
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