Method for gathering vegetable proteins and/or peptides, proteins and/or peptides produced through this method and usage thereof

A plant protein, protein technology, applied in the field of obtaining plant protein and/or peptide, protein and/or peptide produced according to the said, and its use, can solve problems such as burden

Inactive Publication Date: 2009-02-18
EMSLAND STARKE GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extraction requires organic acids, which burden the environment in the effluent

Method used

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  • Method for gathering vegetable proteins and/or peptides, proteins and/or peptides produced through this method and usage thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Binding capacity of 0.4mg protein / cm 2 The surface area is 80m 2 The anion exchanger assembly can bind 320g of protein. 50% of the protein in potato juice is PI at about 10 g / l. After application of 321 potato juice, the capacity was depleted. At a typical flow rate of 300 l / h this takes about 6.5 minutes. Then, the PI protein can be eluted.

Embodiment 2

[0041] Binding capacity of 0.25mg protein / cm 2 The surface area is 80m 2 The cation exchanger module can bind 200g protein. 40% of the protein in potato juice is patatin, at about 8 g / l. It takes 3.3m to fully bind patatin from 11 potato juice 2 membrane. Therefore, at 330m 2 1kg patatin from 1251 potato juice can be adsorbed on the membrane. Then, the patatin can be eluted.

Embodiment 3

[0043] A major advantage of the present invention is the possibility of reusing the membrane adsorbent as well as the rinse and eluent.

[0044] Surface area 15cm 2 The cation exchanger module was loaded with 1.5 ml of a BSA solution having a concentration of 10 mg / ml (BSA=bovine serum albumin). This is slightly below the maximum load of about 20 mg. A protocol to determine long-term stability is performed by loading, rinsing, elution, and rinsing. The washing liquid was 50 mM potassium phosphate buffer pH 7 and the eluent was 1 M NaCl solution in the same buffer. One cycle takes 21.5 minutes. During this time, broadening of the elution peak occurred naturally and up to 65 cycles were possible without clogging of the membrane and without any rupture. If the rinse step is extended by 5 minutes, it is possible to perform more than 100 cycles without cleaning. Blockage occurred at cycle 108. After cleaning with 0.5M sodium hydroxide solution, the membrane was released again...

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Abstract

A method of obtaining vegetable proteins and / or peptides is disclosed that includes the steps of: a) preparing a vegetable starting material containing proteins and / or peptides in an aqueous matrix; b) optionally eliminating solid components from said aqueous matrix and / or clarifying said aqueous matrix; c) isolating the proteins and / or peptides from the aqueous matrix by adsorption on at least one ion exchanger membrane made of a synthetic polymer; d) optionally rinsing the ion exchanger membrane in order to remove impurities; e) desorbing the proteins and / or peptides from the ion exchanger membrane with at least one eluent; f) isolating the proteins and / or peptides from the eluent; and g) optionally drying the isolated proteins and / or peptides; and a protein, peptide and / or mixtures thereof prepared in accordance with the method, and uses thereof.

Description

technical field [0001] The present invention relates to methods for obtaining vegetable proteins and / or peptides, proteins and / or peptides produced according to said methods and mixtures thereof, and uses thereof. Background technique [0002] Both animal and vegetable proteins are known for human consumption. However, animal proteins such as chicken protein and milk proteins such as casein or whey may be involved in problems with BSE, bird flu and other diseases. Animal proteins are also frequently involved in triggering allergic reactions, even if some are not protein-based per se, eg in the case of lactose intolerance. [0003] Plant proteins have problems with genetically modified organisms (GMOs), their nutritional value and as such have the problem of triggering allergic reactions. A well known vegetable protein is soy protein. Another problem often involved with vegetable proteins is that of taste, such as in the case of soybeans, so that their possibilities for us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/18C07K1/34C07K2/00A23J3/14A23K1/14A61K9/48A23L33/00
CPCA23J1/14A23V2002/00A23J1/006A23L1/296A23V2300/30A23K1/1631A23L1/3055A23K20/147A23L33/40A23L33/185A61P3/02A23V2200/30A23V2250/548
Inventor 马丁·罗茨格罗尔德·埃根戈尔
Owner EMSLAND STARKE GMBH
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