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Detection method for measuring amino acid micro-element chelate rate

A detection method and chelate technology, which are applied in measurement devices, color/spectral property measurement, instruments, etc., can solve the problems of determination of chelation rate, chaotic market conditions, unusable titration method, etc., and achieve simple operation and cost saving. , the effect of facilitating the promotion and application

Active Publication Date: 2014-05-14
GUANGZHOU TANKE BIO TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for amino acid trace element chelate samples containing anions that are insoluble in ethanol, the titration method cannot be used to measure the chelation rate, so the titration method also has certain defects.
[0006] At present, there are many amino acid trace element chelate products sold in the market, because the method of detecting chelation rate is not perfect, so most of them are to measure the total content of metal in amino acid trace element chelate products, without considering metal ions Whether it is in a combined or free state, the market situation is relatively confusing. Many products are sold as amino acid trace element chelates, but their essence is nothing more than a mixture of trace elements and amino acids

Method used

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  • Detection method for measuring amino acid micro-element chelate rate
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  • Detection method for measuring amino acid micro-element chelate rate

Examples

Experimental program
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Effect test

Embodiment 1

[0051] Determination method of copper glycinate chelation rate: Weigh 0.5g copper glycinate to make 100mL solution, and measure its ultraviolet-visible absorption spectrum. Then take 0.05g, 0.10g and 0.15g of copper chloride and copper sulfate and also make 100mL solution, measure its ultraviolet-visible absorption spectrum figure, as figure 1 and figure 2 shown. according to figure 1 and figure 2 It can be seen that copper chloride and copper sulfate can be ignored in the determination of the chelation rate of copper glycinate in the range of 0.503g / L to 1.501g / L, so the measurement of the chelation rate of copper glycinate only needs to be performed at the maximum absorption rate of copper glycinate The standard curve is measured at the wavelength. The sample to be tested only needs to weigh a certain amount and dissolve it to measure its absorbance. After calculating the concentration with reference to the standard curve, the chelation rate can be determined. Take 2-9...

Embodiment 2

[0057] The assay method of zinc acid zinc chelation rate: take 0.1g zinc glycinate and be made into 100mL solution, measure its ultraviolet-visible absorption spectrum figure (UV absorption spectrum figure), then take 0.02g zinc chloride and be made into 100mL solution, Measure its ultraviolet-visible absorption spectrum (UV absorption spectrum), and find two wavelengths λ with equal absorbance on the spectrum 1 = 190.35nm and λ 2 =193.70nm, such as image 3 Shown; Then take 7-10mL of the zinc glycinate solution above at these two wavelengths to form a 20mL solution, measure the standard curve of zinc glycinate to obtain the corresponding E, then △E can be obtained. Weigh 0.1g of the zinc glycinate sample to be tested to make 100mL, take 8mL to make 20mL solution, and 1 = 190.35nm and λ 2 = Measure the absorbance at 193.70nm, calculate △A, then calculate the concentration C, and substitute it into the calculation formula of chelation rate to get the chelation rate.

[0058...

Embodiment 3

[0067] Determination method of chelation rate of ferrous glycinate: weigh 0.1g ferrous glycinate to make 100mL solution, measure its ultraviolet-visible absorption spectrum (UV absorption spectrum), then take 0.04g ferrous chloride to make 100mL solution solution, measure its ultraviolet-visible absorption spectrum (UV absorption spectrum), and find two wavelengths λ with equal absorbance on the spectrum 1 = 190.40nm and λ 2 =195.10nm, such as Figure 4 shown; then take 9-12mL of the above-mentioned ferrous glycinate solution under these two wavelengths to form a 20mL solution, measure the standard curve of ferrous glycinate to obtain the corresponding E, then △E can be obtained. Take by weighing 0.13g of ferrous glycinate sample to be tested and make 100mL, get 10mL and make 20mL solution again, at λ 1 = 190.40nm and λ 2 = Measure the absorbance at 195.10nm, calculate △A, then calculate the concentration C, and substitute it into the calculation formula of chelation rate t...

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Abstract

Disclosed is a detection method for measuring the chelation rate of the trace element chelate in amino acid; the main principle comprises the following steps: based on wavelength difference between the ion-state metal ions (such as sulfate, chloride salts and the like) without chelating reaction and the chelated metal irons at the maximum absorption peak in the UV visible light area, the free metal ions and the chelated metal ions can be distinguished; then the concentration of the chelated metal ions is measured so as to caculate the chelation rate. The method is simple to operate and can be used to measure the chelation rates of the trace element chelate in amino acid at different pH values and under different solvent media environments and study the chelation rates of the chelate product under different conditions to provide a basis for the identification of product quality and also provide a reference for the chelate product application under different environments.

Description

technical field [0001] The invention relates to the detection field of the chelation rate of amino acid trace element chelates such as copper glycinate, zinc, iron, etc., and specifically relates to a detection method for measuring the chelation rate of amino acid trace element chelates. Background technique [0002] Amino acid trace element chelate conforms to the mechanism and characteristics of human trace element absorption. It organically combines essential amino acids and trace elements to become a new type of high-efficiency trace element nutrition enhancer with good physical and chemical properties and biological activity. Glycine is the amino acid with the smallest molecular weight, and the chelate formed by its reaction with trace elements is also the amino acid trace element chelate with the smallest molecular weight, which can be better absorbed and utilized by the body. [0003] Amino acid trace element chelate is a compound with a ring structure formed by the c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N33/483
Inventor 舒绪刚李大光林娜妹黄慧民滕冰许祥
Owner GUANGZHOU TANKE BIO TECH