Method for extracting and determining Chinese medicine Schisandra chinensis

A determination method and technology of Schisandra chinensis, which are applied in pharmaceutical formulations, measuring devices, medical preparations containing active ingredients, etc., can solve the problem of lack of scientific standards and basis, lack of determination methods, and low extraction efficiency for extract testing of drug efficacy analysis. To solve the problem, to achieve the effect of scientific and reasonable method, convenient operation, and increase the concentration of active ingredients

Inactive Publication Date: 2009-03-04
山东仙河药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current extraction is usually to pulverize Schisandra chinensis into coarse powder and concentrate under reduced pressure with 40% ethanol to concentrate to a thick paste. The problems of this extraction method are th

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0007] Example one:

[0008] Method for extracting schisandra (liu) extract from schisandra

[0009] The schisandra is crushed into coarse powder, soaked in 75% ethanol for 48 hours, percolated, and added with ethanol at a certain speed (3ml / kg·min) while percolating, percolating until it is basically colorless, and concentrating under reduced pressure to a thick paste.

[0010] Measuring the content of schisandra chinensis extract by high performance liquid chromatography

[0011] The chromatographic conditions are as follows: octadecyl-bonded silica gel is used as the filler; methanol-water (76:24) is used as the mobile phase, the detection wavelength is 250nm, and the theoretical plate number should not be less than 3000 based on the peak of schisandrin alcohol.

[0012] Preparation of reference substance solution Take 2 mg of schisandrin A reference substance, accurately weigh it, and place it in a 100ml measuring flask, dissolve it with methanol, dilute to the mark, and shake...

Example Embodiment

[0015] Embodiment two:

[0016] Method for extracting schisandra (liu) extract from schisandra

[0017] The schisandra is crushed into coarse powder and soaked in 80% ethanol for 48 hours, percolated, and added with ethanol at a certain speed (4ml / kg·min) while percolating, percolating until it is basically colorless, and concentrating under reduced pressure to a thick paste.

[0018] The method for measuring the content of Schisandra chinensis extract using high performance liquid chromatography is the same as in Example 1.

Example Embodiment

[0019] Embodiment three:

[0020] Method for extracting schisandra (liu) extract from schisandra

[0021] The schisandra is crushed into coarse powder and soaked in 85% ethanol for 48 hours, percolated, and added with ethanol at a certain speed (5ml / kg·min) while percolating, percolating until it is basically colorless, and concentrating under reduced pressure to a thick paste.

[0022] The method for measuring the content of Schisandra chinensis extract using high performance liquid chromatography is the same as in Example 1.

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PUM

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Abstract

The invention relates to a method for extracting and mensurating traditional Chinese medicine schisandra chinensis. The coarse powder in schisandra chinensis powder is soaked in and percolated with 75 to 85 percent of alcohol, until the coarse powder is colorless; and decompression and concentration are performed to obtain a schisandra chinensis extractum. The extracted schisandra chinensis extractum utilizes octadecyl silane as a filling agent, and methanol-water as a flowing phase; the detection wavelength is 250nm; and the number of theoretical plates is measured according to the condition that the wave crest of schizandrin is calculated to be less than 3000. Reference substance of the schizandrin is dissolved and diluted with methanol, so as to obtain a reference substance solution. The schisandra chinensis extractum is diluted, filtered, decompressed and evaporated to dryness with the methanol, and added on a treated C18 solid phase extraction column, and eluted with water, the methanol and the methanol; a methanol eluent is collected and diluted to obtain a test sample solution. Two liquids are injected into a liquid chromatograph to be mensurated. The invention integrates an extraction method and a mensuration method for the schisandra chinensis to be applied, thereby increasing the concentration of the effective components of the schisandra chinensis, and ensuring the mensuration and the control of the content of the effective components in the schisandra chinensis medicament by matching dedicated mensuration means.

Description

technical field [0001] The invention relates to a method for extracting Chinese medicine raw materials and a method for measuring the extract, in particular to a method for extracting and measuring the traditional Chinese medicine Schisandra chinensis. Background technique [0002] Modern studies have shown that the traditional Chinese medicine Schisandra is beneficial to Qi and body fluid, astringing the lungs and nourishing the kidneys, relieving diarrhea, astringent essence, and calming the nerves. Pharmacological experiments have proved that Schisandra can regulate the excitatory and inhibitory process of the central nervous system, promote body metabolism, regulate gastric juice and bile secretion, and reduce transaminase levels in convalescent hepatitis patients. Due to the special efficacy of Schisandra chinensis, there are many types of Chinese medicine compositions utilizing Schisandra chinensis as a raw material of Chinese medicine. The current extraction is usual...

Claims

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Application Information

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IPC IPC(8): A61K36/57G01N30/36
Inventor 李保国耿鲁骏
Owner 山东仙河药业有限公司
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