Method for quickly breeding millettia speciosa champ germchit using test tube micro cuttage

Abstract

The invention discloses a method for rapid propagation of beautiful millettia root germchits by using test tube micro-cuttage, which comprises: beautiful millettia root seeds are inoculated into the culture medium for germination and cultivated until the seeds grow into seedlings; the seedlings obtained are cut into stem sections, inserted into the micro-cuttage culture medium and cultivated until the lateral buds come out, then, the lateral buds are cut into stem sections and inserted into a fresh micro-cuttage culture medium for cultivation; after being cut into stem sections, the lateral buds obtained are inoculated into a rooting medium and cultivated until the stem sections take root, thereby obtaining the beautiful millettia root germchits. The technique is simple and easy, has the advantages of simplicity in operation and high propagation rate, solves the problems of small seeds number, low germinating capacity and low propagation rate of germchits propagation by using beautiful millettia root seeds, and realizes long-term large-scale rapid propagation of beautiful millettia root germchits. The technique can obtain high quality beautiful millettia root test tube sprouts in large quantity quickly and continuously, and has strong practicability.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and specifically relates to a method for rapidly propagating ox vigorous seedlings by micro-cutting of test tube stem segments. Background technique [0002] Niu Dali belongs to the Papilionaceae Caulis Spatholobus genus, which was first recorded in the "Herbal Medicinal Properties", and its function is to nourish the deficiency and moisten the lungs, strengthen the tendons and activate the collaterals. For lumbar muscle strain, rheumatoid arthritis, lung heat, lung deficiency cough, tuberculosis, chronic bronchitis, chronic hepatitis, nocturnal emission, leucorrhea, etc. Since the 1970s, it has been used in the production of Chinese patent medicines as raw materials for Zhuangyaojianshen pills and strong fitness capsules, and has been widely used in Guangdong and Guangxi regions; since the end of the last century, folks in Guangdong, Hong Kong, Macao and other places have generally used...

AI Technical Summary

Problems solved by technology

[0003] The traditional breeding method of Niu Dali is seed propagation. The problems of this method are: (1) seed resources are scarce and batch collection is difficult; (2) seeds have a short lifespan and cannot be stored at low temperature, so they cannot be sown regularly to raise seedlings; (3) seeds germinate The rate is low, only reaching about 45%; (4) The root system of seedlings is dominated by taproots, and the number of tubers formed is small, and the yield is low

The existing problems of cutting propagation are: (1) the survival rate of cutting propagation is low, or even all die; (2) cutting resources are in short supply, and mass propagation is difficult

Tissue culture propagation currently adopts the method of callus induction, callus differentiation and proliferation, and adventitious shoot rooting to obtain sterile seedlings. The problems in this method are: (1) the callus induction process takes a long time; (2) the callus The frequency of adventitious bud differentiation from wound tissue was low, and the variation rate of differentiated adventitious buds increased; (3) The elongation speed of adventitious buds differentiated from callus tissue was slow, and the time to reach the rooting standard was long

[0006] The traditional breeding method of Niu Dali is seed propagation. The problems of this method are: (1) seed resources are scarce and batch collection is difficult; (2) seeds have a short lifespan and cannot be stored at low temperature, so they cannot be sown regularly to raise seedlings; (3) seeds germinate The rate is low, only reaching about 45%; (4) The root system of seedlings is dominated by taproots, and the number of tubers formed is small, and the yield is low

The existing problems of cutting propagation are: (1) the survival rate of cutting propagation is low, or even all die; (2) cutting resources are in short supply, and mass propagation is difficult

Tissue culture propagation currently adopts the method of callus induction, callus differentiation and proliferation, and adventitious shoot rooting to obtain sterile seedlings. The problems in this method are: (1) the callus induction process takes a long time; (2) the callus The frequency of adventitious bud differentiation from wound tissue was low, and the variation rate of differentiated adventitious buds increased; (3) The elongation speed of adventitious buds differentiated from callus tissue was slow, and the time to reach the rooting standard was long

These problems have seriously hindered the large-scale production of Niu Dali seedlings, thus unable to meet the market demand for Niu Dali