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Complex enzyme preparation from refined extraction of plant

A compound enzyme preparation and compound cellulase technology, applied in the direction of enzymes, hydrolytic enzymes, biochemical equipment and methods, etc., can solve problems such as long production cycle, pollution, and difficult filtration, so as to shorten the production cycle, reduce production costs, The effect of increasing the yield

Inactive Publication Date: 2009-03-18
宁夏夏盛实业集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional extraction of plant active ingredients adopts hot water, acid, alkali or organic solvent extraction method. Due to the obstruction of the cell wall, the extraction yield is often low, and the viscosity of the plant extract is high, and it is difficult to filter; the mechanical force is ultrafine High cost of crushing; long production cycle; serious pollution by chemical methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Take by weighing according to the following weight ratio:

[0033] Cellulase 35% (of which endoglucanase 50%, exoglucanase 30% and β-glucosidase 20%), pectinase 25% (of which pectin lyase 70%, pectin Esterase 30%), xylanase 20%, glucanase 15% (β-glucanase), glycosidase 5% (wherein rhamnosidase 60% and β-glucosidase 40%).

[0034] Preparation method: the raw material enzymes are respectively filtered through a microfiltration membrane, and then mixed in an automatic mixer in proportion, then filtered through a fine filtration membrane, quantitatively filled, and become a product.

Embodiment 2

[0036] Cellulase 30% (of which endoglucanase 50%, exoglucanase 30% and β-glucosidase 20%), pectinase 15% (of which pectin lyase 70%, pectin Esterase 30%), xylanase 25%, glucanase 25% (β-glucanase), glycosidase 5% (wherein rhamnosidase 60% and β-glucosidase 40%).

[0037] The preparation method is the same as in Example 1.

Embodiment 3

[0039] Cellulase 20% (of which endoglucanase 50%, exoglucanase 30% and β-glucosidase 20%), pectinase 25% (of which pectin lyase 70%, pectin Esterase 30%), xylanase 25%, glucanase 25% (β-glucanase), glycosidase 5% (wherein rhamnosidase 60% and β-glucosidase 40%).

[0040] The preparation method is the same as in Example 1.

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PUM

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Abstract

The invention relates to a complex enzyme preparation refined from plants. The enzyme preparation is prepared from complex cellulase, pectinase, xylanase, glucanase and glycosidase by compounding. According to the characteristics of chemical constituents contained in plant cell walls, the enzymes having respective functions are compounded, wherein the enzymes promote each other and are mutually used by each other through the extremely strong synergic actions among the enzymes; on the one hand, the plant cell walls are damaged to change the permeability of the plant cell walls so that active ingredients are dissolved out to the utmost extent; on the other hand, pectin and various substances (such as pectin, tannin, ash, polysaccharide, stickum and the like) causing turbidity are rapidly degraded so as to eliminate the interference to the extraction of the active ingredients of plants, and to settle extracting solution so that the mixture is easy to filter, thereby greatly shortening the production cycle for factories, reducing the production cost, and increasing the yield.

Description

technical field [0001] The invention relates to the technical field of biological enzyme preparations, in particular to a plant extract compound enzyme preparation for improving the yield of plant active ingredients. Background technique [0002] Enzyme is a special kind of biocatalyst in the form of protein, which is widely used, mainly in beer, fruit juice, textile, feed, leather, alcohol production and other industries. The application in plant extraction, clarification, biotransformation, etc. is a high-tech that has only been gradually applied in recent years. [0003] Most of the active ingredients of plants are wrapped in the cell wall, and the main components of the cell wall are cellulose, hemicellulose, pectin and so on. The traditional extraction of active ingredients of plants adopts hot water, acid, alkali or organic solvent extraction method. Due to the obstruction of the cell wall, the extraction yield is often low, and the viscosity of the plant extract is h...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12N9/26C12N9/46C12N9/24
Inventor 陈龙吴鹏杜小宁
Owner 宁夏夏盛实业集团有限公司
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