Preparation of semicarbazide derivative hapten, antigen and antibody

A semicarbazide and derivative technology, applied in the field of immunochemistry, can solve the problems that there are no technical reports on semicarbazide derivative hapten, antigen and its specific antibody, and achieve high titer and good specificity
CN101407480AInactive Publication Date: 2009-04-15SOUTH CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTH CHINA AGRI UNIV
Publication Date
2009-04-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for preparing semicarbazide derivative hapten, antigen and antibody; semicarbazide derivative hapten is obtained after semicarbazide is condensed with benzaldehyde ammonia aldehyde; the hapten is similar to the structure of semicarbazide derivative, thus showing up the veterinary drug molecular idiosyncratic antigenic determinant, and antigen can be prepared through an active ester method coupling with carrier protein; and by using the antigen immunization, laboratory animals can obtain antibody of high potency and good idiosyncracy aiming at semicarbazide derivative. The antibody can be used in rapidly detecting residual quantity of semicarbazide in animal-derived food, with lower limit of the detection up to 0.09 ng / mL.
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Description

technical field

[0001] The invention belongs to the technical field of immunochemistry, and in particular relates to a method for preparing a hapten, an antigen and an antibody of a semicarbazide derivative, and an application of the prepared hapten, antigen, antibody and antibody. Background technique

[0002] Furacillin, as a nitrofuran drug, has been used to treat and prevent mammalian digestive tract diseases caused by Escherichia and Salmonella. Due to the carcinogenic effect of nitrofuran and its metabolite semicarbazide (SEM), the European Union banned the use of nitrofurans in food animals in 1995, and my country also issued a ban on the use of such veterinary drugs in 2002. At present, nitrofurazone is a mandatory inspection item in international animal-derived food trade, and has become a technical trade barrier for developed countries to restrict exports to third countries. Furacilin is sensitive to light, metabolizes rapidly, and exists in the form of metabolite...

Claims

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