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Anti-EPHRINB2 antiboies and methods using same

A technology of antibodies and uses, applied in the field of molecular biology, can solve problems such as net balance

Inactive Publication Date: 2013-04-24
GENENTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The precise mechanisms controlling the angiogenic switch are not fully understood, but neovascularization of tumor masses is thought to result from a net balance of numerous angiogenic stimulators and inhibitors

Method used

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  • Anti-EPHRINB2 antiboies and methods using same
  • Anti-EPHRINB2 antiboies and methods using same
  • Anti-EPHRINB2 antiboies and methods using same

Examples

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preparation example Construction

[1260] v. Preparation of antibody-drug conjugates

[1261]In the antibody-drug conjugate (ADC) of the present invention, the antibody (Ab) is conjugated to one or more drug moieties (D) via a linker (L), for example, about 1 to about 20 moieties per antibody. drug section. The ADC of general formula I can be prepared through several routes using organic chemical reactions, conditions and reagents known to those skilled in the art, including: (1) the nucleophilic group of the antibody reacts with a divalent linker reagent through a covalent bond to form Ab-L, which subsequently reacts with the drug moiety D; and (2) the nucleophilic group of the drug moiety reacts via a covalent bond with a divalent linker reagent to form D-L, which subsequently reacts with the nucleophilic group of the antibody. Additional methods for preparing ADCs are described herein.

[1262] Ab-(L-D)p I

[1263] A joint may consist of one or more joint components. Exemplary linker building blocks incl...

Embodiment 1

[1337] Example 1: Generation of Anti-EphrinB2 Antibodies

[1338]Various methods are known in the art for generating phage display libraries from which antibodies of interest can be obtained. A synthetic phage antibody library was constructed on a single framework (humanized anti-ErbB2 antibody, 4D5) by introducing diversity within the complementarity-determining regions (CDRs) of the heavy and light chains (Lee, C.V. et al. J Mol Biol 340, 1073 -93 (2004); Liang, W.C. et al. J Biol Chem 281, 951-61 (2006)). Plate panning of naive libraries against His-tagged human EphrinB2 immobilized on maxisorp immunoplates. After four rounds of enrichment, clones were randomly picked and specific binders were identified using phage ELISA. The resulting hEphrinB2-binding clones were further screened with His-tagged mouse EphrinB2 protein to identify cross-species clones. Clone 19 performed well in these assays and was selected for further characterization. For each positive phage clone,...

Embodiment 2

[1339] Example 2: Characterization of anti-EphrinB2 antibodies

[1340] To determine the binding affinity of mouse anti-EphrinB2 mAb, BIAcore TM -3000 (BIAcore, Inc., Piscataway, NJ) for surface plasmon resonance (SRP) measurements. Briefly, the carboxylate was activated with N-ethyl-N'-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) according to the supplier's instructions. Methylated dextran biosensor chip (CM5, BIAcore Inc.). The anti-EphrinB2 antibody was diluted to 5 μg / ml with 10 mM sodium acetate pH 4.8, and injected at a flow rate of 5 μl / min to obtain approximately 500 response units (RU) of the conjugated antibody. Next, 1M ethanolamine was injected to block unreacted groups. For kinetic measurements, two-fold serial dilutions of human or murine EphrinB2-His molecules in PBS containing 0.05% Tween-20 were injected at 25°C at a flow rate of approximately 25 μl / min. Association rates (k on ) and dissociation rate (k off ). ...

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Abstract

The invention provides anti-EphrinB2 antibodies, compositions comprising these antibodies, and methods of using these antibodies.

Description

[0001] related application [0002] This application claims priority under 35 USC §119 to US Provisional Application No. 60 / 760,891, filed January 20, 2006, which is incorporated herein by reference in its entirety. field of invention [0003] The present invention generally relates to the field of molecular biology. More specifically, the present invention concerns anti-EphrinB2 antibodies and uses thereof. Background of the invention [0004] The development of vascular supply is an essential requirement for many physiological and pathological processes. Actively growing tissues such as embryos and tumors require an adequate blood supply. They meet this need by producing pro-angiogenic factors that promote the formation of new blood vessels through a process called angiogenesis. Tube formation of blood vessels is a complex but ordered biological event involving all or many of the following steps: a) proliferation of ECs from pre-existing endothelial cells (ECs) or diffe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/28C07K16/30A61P35/00A61K39/395
CPCC07K16/2866C07K16/18C07K2317/565C07K2317/92A61K2039/505C07K2316/96C07K2317/24C07K2317/73C07K2317/76A61P1/04A61P1/18A61P11/00A61P13/12A61P15/00A61P15/06A61P15/08A61P17/00A61P17/06A61P19/02A61P19/08A61P21/00A61P21/04A61P25/00A61P25/02A61P25/08A61P25/14A61P25/16A61P25/28A61P27/02A61P27/06A61P27/16A61P29/00A61P31/04A61P35/00A61P35/02A61P35/04A61P37/06A61P43/00A61P5/14A61P7/00A61P9/00A61P9/08A61P9/10A61P9/12A61P9/14A61P3/10C07K16/28C07K16/30A61K39/395C07K16/22
Inventor 严民宏吴雁
Owner GENENTECH INC
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