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Spectrum peak complete-superposing two-component mixture simultaneous determination spectral analysis method

A spectroscopic analysis and two-component technology, applied in the field of molecular spectroscopic analysis in analytical chemistry, can solve problems such as the difficulty in the content of two-component substances

Inactive Publication Date: 2009-04-29
杨季冬
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is difficult to simultaneously measure the content of two-component substances at the same wavelength position for this type of two-component substances.

Method used

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  • Spectrum peak complete-superposing two-component mixture simultaneous determination spectral analysis method
  • Spectrum peak complete-superposing two-component mixture simultaneous determination spectral analysis method
  • Spectrum peak complete-superposing two-component mixture simultaneous determination spectral analysis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A two-component mixture to be tested is an inclusion complex formed by β-cyclodextrin (β-CD) and D-tryptophan (D-Try) and L-tryptophan (L-Try), using the present invention An analytical method for the simultaneous determination of a two-component mixture in a medium, comprising the following steps:

[0050] a. Use the standard substances of D-tryptophan (D-Try) and L-tryptophan (L-Try) respectively to prepare D-tryptophan (D-Try) series standard solutions and L-tryptophan (L-Try) series standard solutions, the selected peak position is the measurement wavelength of resonance Rayleigh scattering spectroscopic analysis at 278nm, measure the spectral intensities of the above-mentioned two standard solutions respectively and draw two I ~ C standard curves, will These two standard curves are placed in the same I~C coordinate diagram, obtain the spectrum of the mixture of the two-component D-tryptophan (D-Try) and L-tryptophan (L-Try) to be measured in the present invention ...

Embodiment 2

[0056] The two-component mixture to be tested is the clathrate formed by β-cyclodextrin (β-CD), α-naphthol (α-N) and β-naphthol (β-N). The experiment found that under optimized conditions, after β-cyclodextrin (β-CD) forms an inclusion complex with α-naphthol (α-N) and β-naphthol (β-N), its resonance Rayleigh scattering spectrum At 338nm, β-N has a prominent resonance fluorescence peak, which rises linearly and rapidly with the increase of its concentration; at the same position, α-N has a resonance Rayleigh scattering peak, which rises linearly and slowly with the increase of its concentration; Therefore, 338nm can be used as the measurement wavelength of resonance Rayleigh scattering spectroscopy, and a spectral analysis model for simultaneous determination of α-naphthol (α-N) and β-naphthol (β-N) by resonance Rayleigh scattering spectroscopy can be established. All the other steps are the same as in Example 1.

Embodiment 3

[0058] Wherein the two-component mixture to be tested is an ion association compound formed by the reaction of ammonium molybdate, chlorpromazine hydrochloride (CPZ) and promethazine hydrochloride (PZ). The experiment found that under optimized conditions, ammonium molybdate can react with chlorpromazine hydrochloride (CPZ) and promethazine hydrochloride (PZ) to form ion associations, and the peaks of the resonance Rayleigh scattering (RRS) spectra of both are in the At 365nm, the spectral difference is only in the linear increase of the two RRS spectral peaks with increasing concentration; accordingly, 365nm can be the measurement wavelength of resonance Rayleigh scattering spectroscopy, and the resonance scattering spectrum can be established to simultaneously determine chlorpromazine hydrochloride (CPZ) and Spectral analysis model of promethazine hydrochloride (PZ). All the other steps are the same as in Example 1.

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Abstract

The invention provides a spectral analysis method for synchronously determining a two-component mixture with completely overlapped spectral peaks, which is characterized in that the method establishes a spectral analysis model of the two-component mixture to be determined through the spectral analysis of two-component standard substances in the two-component mixture, then determines the total amount of the two-component mixture to be determined by the same spectral analysis through the model, and calculates to obtain the contents of two components to be determined. The spectral analysis method has the advantages of simple and convenient operation, direct and simple calculation, and reliable results, can be used for spectrofluorimetry, ultraviolet-visible absorption spectral analysis, resonance rayleigh scattering spectral analysis or non-linear second-order scattering spectral analysis and so on, solves the problem of the synchronous determination of the two components with the completely overlapped spectral peaks in molecular spectral analysis, and is particularly suitable for the synchronous determination of a trace amount of two components in environment monitoring, pharmaceutical analysis and quality monitoring.

Description

technical field [0001] The invention relates to molecular spectrum analysis in analytical chemistry, in particular to a spectrum analysis method for simultaneous determination of two-component mixtures with completely overlapping spectral peaks. Background technique [0002] In common molecular spectroscopic analysis methods, for the determination of the content of a two-component mixture, whether it is ultraviolet-visible absorption spectroscopy or fluorescence spectroscopy or resonance Rayleigh scattering spectroscopy, etc., it depends on the peak overlap of the two components. Indicates the overlapping degree of the double peaks (Δλ is the peak distance, Δl is the valley overlapping interval), if the peak overlapping degree is not large, if greater than 1, they can be directly measured respectively; if It is about 0.5 to 1, and a two-component combined calculation method (such as a two-beam equation group method and other spectral intensity method) can be used for sim...

Claims

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Application Information

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IPC IPC(8): G01N21/17G01J3/00
Inventor 杨季冬
Owner 杨季冬