Differentially expressed protein in human glioma and uses thereof

A glioma-specific technology, applied in application, animal/human peptide, microbial measurement/testing, etc., can solve the problem of lack of markers for glioma

Inactive Publication Date: 2009-05-27
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some tumor-related markers have been developed, it is not enough to diagnose tumors only by these markers
Moreover, markers that can specifically diagnose glioma are still lacking

Method used

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  • Differentially expressed protein in human glioma and uses thereof
  • Differentially expressed protein in human glioma and uses thereof
  • Differentially expressed protein in human glioma and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0143] Example 1: Cloning of Human Proteins Upregulated in Glioma Tissue

[0144] Total RNA was extracted from human fetal brain by one-step method of guanidine isothiocyanate / phenol / chloroform. Poly(A) mRNA was isolated from total RNA using Quick mRNA Isolation Kit (product of Qiagene). 2ug poly(A) mRNA was reverse transcribed to form cDNA. Smart cDNA cloning kit (purchased from Clontech) was used to insert the cDNA fragment into the multiple cloning site of the pBSK(+) vector (product of Clontech Company), transform DH5α, and the bacteria formed a cDNA library. The sequences of the 5' and 3' ends of all clones were determined with Dye terminate cyclereaction sequencing kit (product of Perkin-Elmer) and ABI 377 automatic sequencer (Perkin-Elmer). Comparing the determined cDNA sequence with the existing public DNA sequence database (Genebank), it was found that the cDNA sequences of two clones, PP0174B04 and PP1277E03, were new DNA.

[0145] The insert cDNA fragment contain...

Embodiment 2

[0146] Example 2: Homology retrieval of cDNA clones

[0147] The two new human proteins and their coding sequences that are up-regulated in glioma tissues of the present invention were used with the Blast program (Basiclocal Alignment search tool) [Altschul, SF, etc. J.Mol.Biol.1990; 215: 403- 10], in Genbank, Swissport and other databases for homology search, it was found that the identity of the two proteins with other known proteins is not high (less than 50%).

Embodiment 3

[0148] Example 3: Tissue expression profiling studies using cDNA chips

[0149] Gene chip or gene microarray (DNA Microarray) is a new technology that many national laboratories and large pharmaceutical companies are currently developing and developing. It refers to the orderly and high-density arrangement of a large number of target gene fragments on glass, Then use fluorescence detection and computer software to compare and analyze the data, so as to achieve the purpose of rapid, efficient and high-throughput analysis of biological information. The polynucleotide of the present invention can be used as target DNA in gene chip technology for high-throughput research on new gene functions; finding and screening tissue-specific new genes, especially new genes related to diseases such as tumors; disease diagnosis, such as genetic diseases . Its specific method steps have multiple reports in the literature, as can refer to literature DeRisi, J.L., Lyer, V. & Brown, P.O. (1997) S...

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PUM

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Abstract

The invention belongs to the field of biotechnology and discloses a differentially expressed polypeptide in human glioma and a method for encoding a polynucleotide of the polypeptide and producing the peptide by recombinant DNA technology, discloses application of the polypeptide or encoding genes of the polypeptide in preparing a reagent or a regent kit for diagnosing glioma, discloses application of an antagonist resisting the polypeptide in diagnosing and treating diseases, and also discloses the reagent or the regent kit for detecting the glioma.

Description

technical field [0001] The invention belongs to the field of biotechnology, specifically, the invention relates to a new human protein and its encoding nucleic acid which are up-regulated in glioma tissue; the invention also relates to the use of the polypeptide or its encoding nucleic acid; the invention also relates to A reagent or kit for detecting glioma. Background technique [0002] Human genomics research is currently a hot spot in the world. In addition to large-scale sequencing of human chromosomal DNA and expressed sequence sequencing (EST), there is still a lack of high-throughput methods for screening functional genomes starting from functions. [0003] Cancer is one of the major diseases that endanger human health. In order to effectively treat and prevent tumors, people have paid more and more attention to the treatment and detection of tumors. Although some tumor-related markers have been developed, it is far from enough to diagnose tumors only by these mark...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/46C12N15/12C12N15/63C12Q1/68G01N33/50
Inventor 卢亦成姚智强陈菊祥胡国汉骆纯楼美清
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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