Schizophyllum commune protein extract, and preparation method and application thereof

A protein extract and Schizophyllum technology, which is applied to the Schizophyllum protein extract and its preparation, the cultivation method and the medium field of Schizophyllum, can solve the problems of low yield and the like, achieve high yield, increase yield, and prepare simple method effect

Inactive Publication Date: 2009-06-03
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under this liquid culture process, every liter of fermentation liquid contains 200-300g mycelium (wet weight), but its output is still low

Method used

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  • Schizophyllum commune protein extract, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of Schizophyllum hyphae

[0033] 1. Strain activation: transfer the mother strain of Schizophyllum to PDA solid medium (potato starch 20%, glucose 20%, agar 20%, and the rest is water), cultured at 24°C for 10 days, the hyphae are overgrown flat.

[0034] 2. Fermentation culture of Schizophyllum

[0035] (1) Preparation of liquid fermentation medium: Weigh the following components respectively, 60g soluble starch, 55g glucose, 30g wheat flour, 40g corn flour, 6g yeast extract, 10g soybean flour, 6g peptone, 1gKH 2 PO 4 , 0.5gMgSO 4 , 10mg VB 1 , Add these substances into water and dilute the volume to 1000ml, and mix well.

[0036] (2) Preparation of primary strain: cut the flat hyphae obtained in step 1 into small pieces less than 1mm, and transfer the Schizophyllum hyphae into a 100ml fermentation medium at an inoculum of 0.4g hyphae / 100ml In a 500ml shake flask, culture in a shake flask at 24° C. and 200 rpm for 6 days to obtain a first-level strain...

Embodiment 2

[0039] Example 2 Preparation of Schizophyllum hyphae

[0040]1. Strain activation: transfer the mother strain of Schizophyllum to PDA solid medium (potato starch 20%, glucose 20%, agar 20%, the rest is water), culture at 26°C for 10 days, and the hyphae are overgrown flat.

[0041] 2. Fermentation culture of Schizophyllum

[0042] (1) Preparation of fermentation medium: Weigh the following components respectively, 40g soluble starch, 40g glucose, 20g wheat flour, 20g corn flour, 3g yeast extract, 5g soybean flour, 2g peptone, 0.1gKH 2 PO 4 , 0.5gMgSO 4 , 10mg VB 1 , Just add these substances into 1000ml water and mix well.

[0043] (2) Preparation of primary strain: cut the plate hyphae obtained in step 1 into small pieces less than 1mm, and transfer the Schizophyllum hyphae into a 100ml fermentation medium at an inoculum of 0.6g hyphae / 100ml In a 500ml shake flask, culture in a shake flask at 26°C and 240rpm for 6 days to obtain a first-level strain (concentration of 4.2g / 100ml)....

Embodiment 3

[0046] Example 3 Preparation of Schizophyllum hyphae

[0047] 1. Strain activation: transfer the mother species of Schizophyllum to PDA solid medium (potato starch 20%, glucose 20%, agar 20%, the rest is water), cultured at 28°C for 10 days, the hyphae are overgrown flat.

[0048] 2. Fermentation culture of Schizophyllum

[0049] (1) Preparation of fermentation medium: Weigh the following ingredients respectively, 60g soluble starch, 55g glucose, 30g wheat flour, 40g corn flour, 6g yeast extract, 10g soybean flour, 6g peptone, 1gKH 2 PO 4 , 0.5gMgSO 4 , 10mg VB 1 , Add these substances to water and dilute to 1000ml and mix well.

[0050] (2) Preparation of first-class strains: cut the plate hyphae obtained in step 1 into small pieces less than 1mm, and transfer the Schizophyllum hyphae into a 100ml fermentation medium at an inoculum of 0.8g hyphae / 100ml In a 500ml shake flask, cultured in a shake flask at 28°C and 260rpm for 6 days to obtain a first-class strain (concentration of ...

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Abstract

The invention discloses a Schizophyllum commune protein extract and a preparation method and application thereof. The preparation method comprises the steps of leaching Schizophyllum commune mycelium powder in a phosphate buffer, and centrifuging to obtain a crude Schizophyllum commune mycelium extractive solution; and adding ammonium sulfate to the crude Schizophyllum commune mycelium extractive solution, standing for 6-12 h, and centrifuging to obtain the Schizophyllum commune protein extract. The invention also discloses a culture method of Schizophyllum commune and a fermentation culture medium for Schizophyllum commune. The Schizophyllum commune protein extract has good prevention and control effects on tobacco mosaic virus (TMV), and inhibition rate to TMV is over 70%. The Schizophyllum commune protein extract belongs to natural extracts, is harmless to human and environment, and belongs to environment-friendly pesticides. The preparation method has the advantages of simple process, low cost, and applicability to large-scale production. In addition, the yield of Schizophyllum commune is high by using the fermentation culture medium.

Description

Technical field [0001] The invention belongs to the field of plant disease prevention and control, and specifically relates to a Schizophyllum protein extract and its preparation method and application; in addition, it also relates to a Schizophyllum culture method and culture medium. Background technique [0002] Plant virus diseases are similar to human cancers, and there is currently no very effective prevention and control method. Tobacco mosaic virus (TMV) is one of the most economically important viruses. It can infect more than 300 plants such as Solanaceae, Cucurbitaceae, and Cruciferae, and cause serious harm. Plant virus disease prevention and control are usually Relying on breeding and chemical agents to solve the problem, but the effect is not ideal. The former is limited by the source of disease resistance genes, while the latter is ineffective and will cause environmental pollution and other problems. In recent years, with people's attention to the environment, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P3/00C07K1/14C07K2/00C12N1/14C12R1/645
Inventor 周莹李兴红严红燕继晔卢娜
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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