Application of iridoid in preparing anti-tumor medicine

A technology of iridoids and anti-tumor drugs, which is applied in the field of medicine, and can solve the problems of insufficient comprehensive and systematic research on chemical components and insufficient thorough research on iridoid components.

Inactive Publication Date: 2009-06-03
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the research on spider incense has reached a certain scale, the research on its chemical component

Method used

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  • Application of iridoid in preparing anti-tumor medicine
  • Application of iridoid in preparing anti-tumor medicine
  • Application of iridoid in preparing anti-tumor medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: Preparation of iridoids

[0021] 25kg of dried spider incense medicinal material, crushed and extracted by percolation with 95% ethanol, the extract was decompressed to recover ethanol to a volume of 20L, filtered, the filtrate was extracted with petroleum ether, chloroform, ethyl acetate and n-butanol in sequence, and the extracted parts were collected And concentrated into an extract, wherein 120g of the extract is extracted with chloroform, and 180g of the extract is extracted with ethyl acetate. The obtained chloroform extraction fraction and ethyl acetate extraction fraction were subjected to silica gel column chromatography, gradient elution was carried out with different proportions of chloroform-methanol, and thin-layer chromatography was used for detection, and combined with Sephadex LH-20 and preparative liquid chromatography, the compounds were analyzed. Purified to obtain 42 compounds, using UV, MS, 1 H NMR, 13 C NMR, 2D NMR and other methods...

Embodiment 2

[0026] Embodiment 2: Cytotoxic activity test of iridoid compounds

[0027] 1. Experimental materials

[0028] 1.1. Test samples

[0029] After dissolving 21 iridoid compounds as described in Table 1 with DMSO (Merck) respectively, add PBS (-) to form a solution or a uniform suspension of 1000 μg / ml, and then use DMSO-containing PBS (- )dilution.

[0030] 1.2. Cell lines

[0031] A549 (human lung cancer cells)

[0032] PC3 (human prostate cancer cells)

[0033] HCT-8 (human colon adenocarcinoma cells)

[0034] Bel 7402 (human liver cancer cells)

[0035] 1.3. Culture medium

[0036] RPMI1640+15%NBS+double antibody

[0037] 1.4. Other materials

[0038] Fully automatic microplate reader: model: WellscanMK-2, manufacturer: Labsystems

[0039] Imported 96-well culture plates, etc.

[0040] 2. Test method

[0041] MTT method: 4-6×10 4 cells / ml cell suspension 100μl, set at 37°C, 5% CO 2 Inside the incubator. After 24 hours, add the sample solution, 10 μl / well, set up...

Embodiment 3

[0044] Example 3 Curative effect experiment of iridoid compounds on mouse S180 sarcoma (solid type)

[0045] 1. Experimental materials

[0046] 1.1 Test sample

[0047] As described in Table 1, 21 iridoid compounds were respectively dissolved with a small amount of Tween-80 and then formulated into solutions with 0.5% CMC.

[0048] 1.2 Animals

[0049] Strain: Kunming mouse

[0050] Source: Provided by the Experimental Animal Center of Second Military Medical University.

[0051] Certificate number: Shanghai Donghe Zhengzi No. 107

[0052] Weight: 18-20g

[0053] Gender: female.

[0054] 1.3 Transplanted tumors

[0055] The mouse S180 sarcoma was maintained by the Shanghai Institute of Pharmaceutical Industry.

[0056] 2. Experimental method

[0057]Take the well-growing mouse S180 sarcoma ascites, dilute it with normal saline at 1:4, inoculate 0.2ml under the skin of each mouse armpit, divide into random groups, and divide them into control group and cyclophosphamide...

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Abstract

The invention belongs to the medication technical field, and in particular relates to application of iridoid in preparing anti-tumor medicine. Animal experiments proves the compound has obvious inhibiting effect on various cancer cells without toxic effect or side effect, so that the compound can be used for preparing the anti-tumor medicine. The invention further provides a medicine composition using the iridoid as an active composition for treating tumor. The compound can either be extracted from plants, or obtained by a chemical synthesizing method.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of iridoid compounds in the preparation of antitumor drugs. Background technique [0002] Tumor is one of the main causes of human death. The Ministry of Health of China announced the main causes of death of urban and rural residents in 2006. Statistics show that malignant tumors have become the leading cause of death. It can be seen that the prevention and treatment of tumors is very urgent. Drug therapy is one of the main treatments for tumors. At present, although dozens of anti-tumor drugs have been developed, they can effectively prolong the life of patients or improve the quality of life of patients. Some of these anti-tumor drug treatments have very significant curative effects, such as drug treatment of acute leukemia in children. However, the research and development of tumor drugs is still facing great challenges. For example, most anti...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P35/00
Inventor 张卫东林生单磊苏娟柳润辉李慧梁沈云亨徐希科
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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