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Method for recovery of lipase activity

A recovery method, lipase technology, applied in the directions of microorganism-based methods, fatty acid esterification, biochemical equipment and methods, etc., can solve problems such as decreased lipase activity

Active Publication Date: 2009-07-22
THE NISSHIN OILLIO GRP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, since lipase activity decreases once lipase is immobilized on a carrier, various technologies using lipase powder are being gradually developed

Method used

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  • Method for recovery of lipase activity
  • Method for recovery of lipase activity
  • Method for recovery of lipase activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) 1 Kg of Lipozyme TL-IM (average particle size 800 μm) produced by Novozymes A / S Company was pulverized at 17600 rpm using a steel pin mill (Fine impact mill 100 UPZ) produced by Hosokawa Micron Corporation. The particle size of the crushed lipase was measured with a particle size distribution analyzer LA-500 manufactured by Horiba Manufacturing Co., Ltd., and the average particle size was 13.8 μm. 1 kg of cellulose powder (Nippon Paper Chemicals Co., Ltd: average particle size 30 μm) as a filter aid was added to the powder to prepare a lipase powder composition.

[0047] (2) Add 90 g of rapeseed decolorizing oil and 10 g of ODO (manufactured by Nisshin Oleo Group Co., Ltd.: medium-chain fatty acid triglycerides) to 5 g of the lipase composition obtained in this way, stir at room temperature for 24 hours, and filter Recover the lipase composition. The transesterification activity of the lipase composition was measured by the following method. When the activity of Lipozym...

Embodiment 2

[0059] (2-1) To 5g of the lipase composition obtained in (1) of Example 1, 90g of rapeseed decolorizing oil and 10g of ODO (manufactured by Nisshin Oriyo Group Co., Ltd.) were added and stirred at 60°C for 2 hours , Filter and recover the lipase composition. The transesterification activity of the lipase composition was measured by the same method as in Example 1, and its relative activity was 557.

[0060] (2-2) Add 1.2% by mass of the lipase composition obtained in (2-1) above to 100 g of soybean oil and 25 g of extremely hardened soybean oil (manufactured by Hengsen Oil Industry Co., Ltd.), and react at 70°C After 120 hours, the lipase composition was recovered by filtration. In the same manner as in Example 1, the lipase activity (2-2a) of a part of the recovered lipase composition was measured. Disperse the previously recovered lipase composition in acetone. After filtering, the filter residue is recovered and dispersed in 50 g of rapeseed decolorizing oil: ODO (manufactured ...

Embodiment 3

[0064] Add 5% by mass of Lipozyme TL-IM (immobilized lipase: Novozymes) to 85 g of rapeseed decolorizing oil (manufactured by Nissin Oriyo Group Co., Ltd.) and 15 g ODO (manufactured by Nissin Orion Group Co., Ltd.) Produced by A / S Company), stirred at 60°C for 19 hours to carry out the transesterification reaction. The transesterification rate over time was determined, and the progress of the reaction was confirmed. In the transesterification reaction, gas chromatography was used to analyze the glyceride composition, and the ratio of the transesterification reactant in the measurement sample was calculated by calculation.

[0065] The above-mentioned immobilized lipase after the reaction is filtered and recovered, and the recovered immobilized lipase is repeatedly used in the transesterification reaction. Repeat the reaction several times, confirm the change of the reaction rate by the relative ratio, and show it in image 3 (3-1).

[0066] The above-mentioned immobilized lipase ...

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Abstract

Disclosed is a method for recovery of a lipase activity, which comprises subjecting a lipase powder composition to an esterification or transesterification reaction and then washing the composition with triacyl glycerol, wherein the lipase powder composition comprises: a lipase derived from a microorganism belonging to the genus Thermomyces which is immobilized on a carrier, or a pulverized product of the lipase which has an average particle diameter of not smaller than 1 mum and smaller than 300 mum and is immobilized on a carrier; and a filter aid. The method enables to recover a decreased activity of a lipase with high efficiency.

Description

Technical field [0001] The present invention relates to a method for restoring lipase activities such as various esterification capabilities and transesterification capabilities of a specific immobilized lipase or lipase powder composition, and esterification using the restored immobilized lipase or lipase powder composition Reaction, transesterification method of fats and oils, etc. Background technique [0002] Lipases are widely used in esterification reactions of various carboxylic acids such as fatty acids and alcohols such as monohydric or polyhydric alcohols, transesterification reactions between multiple carboxylic acid esters, and the like. Among them, the transesterification reaction includes the modification of animal and vegetable oils and fats, and is a very important technology as a method for producing various fatty acid esters, sugar esters, or steroids. If lipase, which is an oil and fat hydrolase, is used as a catalyst for these reactions, the transesterificatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/20C11C3/02C11C3/10C12P7/64C12R1/01C12P7/6454C12P7/6458
CPCC11C3/02C12N9/20C11C3/10C12P7/6454C12P7/62C12N11/14C12P7/6458C12P7/64
Inventor 根岸聪铃木顺子山内良枝
Owner THE NISSHIN OILLIO GRP LTD
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