Capillary array analyzer by rotating scanning

Abstract

The invention belongs to the bioanalysis technical field, and relates to a rotational scanning capillary array analyzer. The analyzer comprises a laser light source, a scanning reflector, a scanning objective, a detection window fixing device, a capillary sampling end, a capillary exhaust liquid end, a light beam steering system, a fluorescence spectroscope, a fluorescence color filter, an optical detector and a scanning central shaft, wherein, a plurality of capillaries are arranged around the scanning central shaft to form a cylindrical array, and the capillary array is fixedly arranged in the detection window fixing device; laser beam of the light source sequentially passes through the fluorescence spectroscope, the scanning reflector, the light beam steering system and the scanning objective, and the scanning objective converges the laser beam on the detection window; a biological sample marked with fluorescence in the capillary is activated by the laser, irradiated fluorescence passes through the scanning objective, the light beam steering system, the fluorescence spectroscope and the fluorescence color filter, and is detected by the optical detector. The rotational scanning capillary array analyzer has the advantages of high flux, high sensitivity, large fluorescence collection angle and good performance.

Description

Rotating Scanning Capillary Array Analyzer technical field The invention belongs to the technical field of biological analysis, in particular to a rotary scanning capillary array analyzer. Background technique Capillary electrophoresis is under the action of an electric field, the sample migrates in the capillary, and different components in the sample have different migration speeds to achieve separation, and detect at the detection window in the capillary, and analyze the sample through the detected signal. This technology is widely used in biochemical analysis, such as DNA and protein separation and analysis. It has high separation efficiency, low sample consumption and easy automation. It is widely used in DNA sequencing, biological and clinical research, forensic identification, environmental protection, analytical chemistry and food. It has a wide range of applications in detection and other fields. The detection of electrophoretic samples mainly includes isotope la...

AI Technical Summary

Problems solved by technology

In this way, the capillaries need to be arranged in a circle, the system volume is large, and the lens size is required to be small, and because the lens is located inside the cylindrical surface of the capillary array, the arrangement of the capillary array is concave to the lens, and the cylindrical surface of the array is limited by volume

Therefore, the working distance of the lens is required to be large, the angle of fluorescence collection is small, and the detection sensitivity of the instrument is limited.

Images