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Chemiluminescence method for detecting chlorophyll alpha

A technology of chemiluminescence and chlorophyll, which is applied in chemiluminescence/bioluminescence, analysis through chemical reactions of materials, etc., can solve the problems such as the unregulated daily intake of chlorophyll

Inactive Publication Date: 2009-07-29
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

3. No toxic side effects, long-term consumption safety, high reliability, FAO / WHO (1985) did not stipulate the per capita daily intake of chlorophyll
However, the detection of chlorophyll activity in vitro has not been reported.

Method used

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  • Chemiluminescence method for detecting chlorophyll alpha
  • Chemiluminescence method for detecting chlorophyll alpha
  • Chemiluminescence method for detecting chlorophyll alpha

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] 1. Material: organisms containing chlorophyll a.

[0025] 2. Instruments and equipment: 1 / 10,000 electronic balance; flow injection chemiluminescence analysis test system;

[0026] UV-visible spectrophotometer.

[0027] 3. Reagents:

[0028] (1) Chlorophyll sample stock solution: taking stevia leaves as an example, take 100g of stevia leaves that have been washed and dried, add 500mL of acetone with a volume percentage concentration of 80%, ultrasonically oscillate for 30min and soak overnight, and filter under low temperature , measure the quasi-supernatant volume, add dioxane in a volume ratio of 1: 7 by dioxane: acetone extract, and then press water: acetone extract in a volume ratio of 1: 6, stirring Add distilled water drop by drop until precipitation occurs, put it in a refrigerator at -20°C for 30 minutes, and centrifuge at 1500×g for 5 minutes to obtain a dark blue-black powdery mixture of chlorophyll a and chlorophyll b. Dissolve the precipitate in 60 mL Abs...

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PUM

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Abstract

The invention discloses an analysis method for flow injection chemiluminescence chlorophyll a determination by a potassium ferricyanide-luminol system. The method comprises the processes of optimization of flow injection chemiluminescence analytic system test parameters establishment of a method for determining chlorophyll a by the potassium ferricyanide-luminol system and the like. The chlorophyll a can be determined by the method, but chlorophyll b can not be determined by the method. Mass concentration of the chlorophyll a within 0.5-10.0mg / L is subject to a good linear relation with a sensitization rate, a regression equation thereof is I=23.244C-4.2218(R=0.9991), and detection limit of the method is 0.13mg / L. Standard chlorophyll a solution with the concentration being 5.0mg / L is subject to 11 precision tests in parallel determination and repeated tests divided into 6 partial determinations, and RSD thereof is 1.17% and 3.74%. The method can rapidly and flexibly detect the chlorophyll a.

Description

technical field [0001] The invention relates to a chemiluminescent method for detecting chlorophyll a. Background technique [0002] Chlorophyll a mainly has the following characteristics: 1. In the photosynthesis of plants, most of chlorophyll a and all chlorophyll b are chlorophylls involved in absorbing and transmitting light energy. And another part of chlorophyll a is located on the photoreaction system complex and acts as a photoreaction center. They absorb the energy transferred from other antenna pigments to promote the photochemical reaction. Whether it is an antenna pigment or a reaction center pigment, chlorophyll is bound to a specific protein to form a pigment-protein complex to perform its function. 2. The color of fruits and vegetables is an important factor in the quality of products, and it is also the basis for testing the maturity and aging of fruits and vegetables. The color not only reflects the freshness of fruits and vegetables, but also stimulates pe...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 郝再彬关锦毅杨丹李子院李海云
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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