Method for detecting mould fungus inhibition effect of leather material and product thereof

A detection method, mold technology, applied in the field of detection of the effect of mold inhibition, can solve the problems of long experiment period, cumbersome steps, and far differences in mold growth conditions, etc., and achieve the effect of short detection period, strong practicability, and simple operation

Inactive Publication Date: 2009-08-05
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, it is cumbersome to add and apply Cha's culture medium in the center of the stained skin piece, and it is easy to solidify under the experimental conditions, and it is not easy to spread; 15 days of cultivation, the experiment period is longer; drop enrichment on the sample The nutrient medium is far from the mold growth conditions of leather materials and their products in actual use

Method used

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  • Method for detecting mould fungus inhibition effect of leather material and product thereof
  • Method for detecting mould fungus inhibition effect of leather material and product thereof
  • Method for detecting mould fungus inhibition effect of leather material and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Preparation of spore suspension

[0030] Add 10 ml of sterilized 0.85% NaCl solution containing 0.1% Tween80 to a slant of Penicillium chrysogenum cultured for 5 days, and slowly scrape off the spores from the surface of the culture with an inoculation loop. Gently shake the liquid to disperse the spores without separating the mycelia, and slowly pour the mold suspension into a flask containing a small amount of glass beads, shake the spore suspension well, and then wipe it with a sterile single-layer cotton cloth or glass fiber cotton filter, adjust the spore concentration to 1 × 10 6 ~10×10 6 cfu / ml.

[0031] (2) Preparation and operation of test samples

[0032] The sample is cut into (2.5±0.5)cm×(3.8±0.5)cm;

[0033] After the test sample is sterilized by ultraviolet irradiation, it is placed on the culture medium poured into the plate. Use a pipette or a sterile pipette to take 0.2ml of spore suspension or mixed spore suspension and evenly drop it on the s...

Embodiment 2

[0037] (1) Preparation of spore suspension

[0038] Add 10 ml of sterilized 0.85% NaCl solution containing 0.1% Tween80 to a slant of Rhizopus niger that has been cultured for 5 days, and slowly scrape off the spores from the surface of the culture with an inoculation loop. Gently shake the liquid to disperse the spores without separating the mycelia, and slowly pour the mold suspension into a flask containing a small amount of glass beads, shake the spore suspension well, and then wipe it with a sterile single-layer cotton cloth or glass fiber cotton filter, adjust the spore concentration to 1 × 10 6 ~10×10 6 cfu / ml.

[0039] (2) Preparation and operation of test samples

[0040] The sample is cut into (2.5±0.5)cm×(3.8±0.5)cm;

[0041] After the test sample is sterilized by ultraviolet irradiation, it is placed on the culture medium poured into the plate. Use a pipette or a sterile pipette to take 0.2ml of spore suspension or mixed spore suspension and evenly drop it on ...

Embodiment 3

[0045] (1) Preparation of spore suspension

[0046] Add 10 ml of sterilized 0.85% NaCl solution containing 0.1% Tween80 to a slant of Penicillium chrysogenum and Aspergillus niger that has been cultured for 5 days, and slowly scrape off the spores from the surface of the culture with an inoculation loop. Gently shake the liquid to disperse the spores without separating the mycelia, and slowly pour the mold suspension into a flask containing a small amount of glass beads, shake the spore suspension well, and then wipe it with a sterile single-layer cotton cloth or glass fiber Cotton filter, mix each single mold spore suspension by equal volume to make a mixed mold spore suspension, and adjust the concentration of the mixed spore liquid to 1×10 6 ~10×10 6 cfu / ml.

[0047] (2) Preparation and operation of test samples

[0048] The sample is cut into (2.5±0.5)cm×(3.8±0.5)cm;

[0049] After the test sample is sterilized by ultraviolet irradiation, it is placed on the culture me...

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Abstract

The invention relates to a leather substance and a detection method of the mould inhibition effect of a product thereof. The detection method comprises the following steps: firstly, adding NaCI solution or PBS buffer solution into a mould inclined plane culture and adjusting the concentration of spore suspension into 1*10 to 10*10 cfu / ml to prepare mould spore suspension or mixing various single mould spore suspension according to equivalent volume to prepare mixed mould spore suspension; secondly, placing the leather substance and the product thereof after being radiated by ultraviolet radiation into a culture medium and dropping the spore suspension on the leather substance to culture for 3 to 5 days at the temperature of 28 DEG C plus or minus 1 DEGC; and thirdly, carrying out visual evaluation and microscopic examination to the growth degree of epiphyte on a sample by using a microscope*50 if necessary. The detection method has the advantages of simple operation, shorter detection period and strong practicability; only mould spore is inoculated on the surface of the sample, so that the effect caused by the excessive growth of mycelium due to more mould spores is voided, the natural environment is simulated, and the growth situation of mould is analyzed.

Description

technical field [0001] The invention belongs to the field of detection of mold inhibitory effects of materials and products thereof, in particular to a detection method of mold inhibitory effects of leather materials and products thereof. Background technique [0002] Leather is a fixed and durable substance transformed from animal skin through a series of physical and chemical treatments. The main component is cross-linked protein. Many of the raw and auxiliary materials used in the tanning process also contain protein and sugar. , fat and other nutrients, so leather and its leather products contain rich nutrients that can be utilized by microorganisms. As long as the external temperature and humidity conditions are suitable, microorganisms will grow and reproduce, seriously affecting the quality of leather and its products. The most important microorganism affecting leather is mold, which produces mildew. Therefore, the antibacterial and antifungal properties of leather ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/645C12R1/685C12R1/845C12R1/82C12R1/785
Inventor 邓云霞杜鹃罗艳钟毅汪姣宁
Owner DONGHUA UNIV
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