Method for treating diabetic vascular complications

A technology for diabetic blood vessels and complications, applied in the preparation of drugs for the treatment of diabetic blood vessel complications, and the field of extracts of Dioscorea species for treating diabetic blood vessel complications

Active Publication Date: 2009-08-05
NATIONAL YANG MING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no studies have been conducted on the use of yam extract in the treatment of diabetic vascular complications

Method used

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  • Method for treating diabetic vascular complications
  • Method for treating diabetic vascular complications
  • Method for treating diabetic vascular complications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Preparation of crude extract (PH) of Dioscorea species

[0048] Peeled tubers of Dioscorea sp. were immersed in an alcohol-based solution in the presence of 1% acetic acid. After stirring, the mixture solution was left standing overnight. The soluble fraction obtained by this process was lyophilized to obtain a crude extract of Dioscorea sp. (PH).

[0049] Specifically, 1.4 kg of peeled tubers of Dioscorea sp. were cut into small pieces and immersed in a 1% acetic acid solution. The pellet was soaked in the acetic acid solution overnight and the supernatant was removed. The pellets were lyophilized prior to extraction.

[0050] The lyophilized pieces are milled and mixed with about 30% to about 90% methanol (eg, about 40% methanol) as about 1.5 L to about 2.5 L of solvent in the presence of about 1% acetic acid solution. The mixture was left overnight and filtered by vacuum filtration to obtain a filtrate. The filtrate was then lyophilized to obtain a cr...

Embodiment 2

[0052] Example 2: Analysis of crude extracts (PH) of Dioscorea species

[0053] The crude extract obtained in Example 1 (including methanol and ethanol extract) was used with two pumps (LC-10AT), a SPD-10A programmable adjustable wavelength UV / Vis photodiode array detector, a SCL-10A System controller and a Shimadzu HPLC system equipped with a C-18 column (Supelcosil, 250mm x 4.6mm, and with 5 μm packing; Supelco, Bellefonte, USA) with a guard column (35mm x 4.6mm, and with 5 μm packing; Supelco) (Class VP series with Class VP version 6.12 software; Shimadzu, Kyoto, Japan) Analysis was performed by HPLC.

[0054] HPLC samples (10 mg / mL) at pH were prepared in 20 μL aliquots using a mobile phase containing 88% water (mobile phase A) and 12% methanol (mobile phase B) and loaded to min equilibrated columns. All organic solvents were HPLC grade and were filtered with 0.22 μm filter and degassed before use. The total run time of the HPLC method was set at 20 min. Elution profil...

Embodiment 3

[0058] Embodiment 3: the preparation of the mouse feed that contains crude extract (PH)

[0059] Purina Chow 5001 (a commercially available mouse chow) was ground into a powder. The lyophilized crude extract PH was added to the milled feed in an amount replacing the same amount of said milled feed to form a feed mixture. The feed mixture was uniformly mixed with distilled water, reshaped by extrusion, baked in a microwave oven with appropriate power for 2 min, and frozen at -70°C after cooling to room temperature. After lyophilization, the feed blend was made into pellets very similar to the original Purina Chow feed. The pellets were stored in a -20°C freezer. On the day of feeding, the pellets were warmed to room temperature and sterilized on a sterile bench by irradiation with UV lamps. Feed mixes were prepared with different concentrations of crude extract.

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Abstract

The present invention provides a method for treating diabetic vascular complications including administering to a subject in need thereof a therapeutically effective amount of an extract product of a Dioscorea species. The extract product preferably is prepared by a process which involves (a) extracting a tuber of the Dioscorea species with an alcohol-based solvent in the presence of an acetic acid solution to form an extract composition, (b) subjecting a resultant product obtained in step (a) to obtain a soluble fraction, and (c) removing solvent from the soluble fraction obtained in step (b) to obtain the extract product.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Patent Application No. 60 / 804,656, filed June 14, 2006, and U.S. Nonprovisional Patent Application No. 11 / 582,894, filed October 18, 2006, which are hereby incorporated by reference in their entirety. into this article. Background of the invention [0003] The present invention relates to the drug treatment of diabetic vascular complications, in particular to a method for treating diabetic vascular complications with the extract of Dioscorea species. [0004] Nonenzymatic glycosylation (Maillard reaction) has been implicated in the pathology of diabetic vascular complications. Recent immunohistochemical studies have shown that the formation of advanced glycation end products (AGEs) is enhanced in diabetic subjects (Liu et al., Diabetes. 48:2074-2082, 1999). AGE formation is known to itself alter the structural and functional properties of tissue proteins. In additio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/00C12N15/82
Inventor 吴荣灿
Owner NATIONAL YANG MING UNIVERSITY
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