Method for preparing optical pure (R)-2-octanol by immobilized microorganism

Abstract

The invention discloses a method for preparing high optical pure (R)-2-octanol by a microbiological method, and a microorganism thereof. An immobilized-cell synthesis method of the invention has the advantages that: 1, an immobilization process is simple, and immobilized yeast cells have certain poison resistance, have excellent catalytic effect and can be repeatedly used in a reaction system; 2, the immobilized cells prepared by taking non-polar macroporous absorbent resin as a carrier are good in elasticity, strong in tolerance to temperature and pH, high in mechanical strength, stable in enzyme activity and long in half-life period; 3, as reaction is carried out in two phases of organic solvent/buffer solution, the toxic action of the organic solvent on the cells can be reduced, and the catalytic activity of the organic solvent is improved; and 4, the preparation of the optical pure (R)-2-octanol through the immobilized yeast cells is simple in process, free from need for no expensive coenzyme, mild in condition, short in reaction time, nearly free from byproducts, up to 90.25 percent e.e. in optical purity, simple in subsequent treatment, easy in product separation and friendly to environment.

Description

technical field [0001] A method for preparing optically pure (R)-2-octanol by microorganisms belongs to the technical field of microorganisms asymmetrically catalyzing prochiral compounds to synthesize optically pure compounds. Background technique [0002] 2-octanol, also known as 2-octanol, is often used in fine organic synthesis and can be used to produce chiral chemical materials and fragrances. (R)-2-octanol is an important chiral material for the preparation of high-performance liquid crystals and liquid crystal devices. It can greatly improve the quality of liquid crystals and change low-grade liquid crystals (achiral) into high-grade liquid crystals (chiral). At the same time, it is also an important intermediate for the synthesis of many optically active drugs and pesticides. Currently known methods for preparing optically pure 2-octanol mainly include three approaches: (1) chemical methods. Usually, optically pure 2-octanol is prepared by chemical method, which r...

AI Technical Summary

Problems solved by technology

Since microbial cells contain a complete enzyme system, for example, yeast cells contain rich oxidoreductase systems, which can realize the reduction and regeneration of coenzymes without adding expensive coenzyme factors, and there are few by-products, the yield is greatly improved, and the subsequent process is simplified. treatment process, but the cells cannot be reused, and the separation of products is still too complicated [Li Yongning et al., Pharmaceutical Biotechnology. 2006, 13(6): 446-450; Hu Jian et al., Acta Chemical Industry. 2006, 57(10): 2383-2387]