Method for preparing sea cucumber collagen small peptide freeze drying powder extracted from sea cucumber decoction liquor
The technology of sea cucumber collagen and freeze-dried powder is applied in the field of sea cucumber deep processing, which can solve the problems of natural environment pollution, waste of sea cucumber regeneration resources, etc., and achieve the effects of reducing production environment pollution, facilitating digestion and absorption, and high purity of small peptides.
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Embodiment 1
[0031] Collect 1000kg of cooking liquid produced in sea cucumber processing, and circulate it into a vacuum thermal diaphragm low-temperature concentrator, control the vacuum degree of 1.0Mpa, heat at 40°C, and concentrate it into 600kg of sea cucumber boiling liquid concentrate after 2 hours.
[0032] Use a material pump to transport to a 1000L vacuum bioreactor, adjust the pH to 7, and heat to 40°C with steam. Add 120g of compound protease (protamex produced by Novozymes Functional Food Industry, Denmark); 180g of 1398 neutral protease; 120g of 1203 lipase; 240g of Vc, then seal it, vacuumize 100Pa and stir for 6 hours, and use a material pump to input 100 mesh to 300 Objective Duplex filter to separate the impurities in the liquid to obtain 600kg compound enzymolysis solution. Then use 0.1 μPa vacuum suction to the 1000L enzyme modification reaction kettle, adjust the pH value to 7, control the temperature at 40°C, add 180g of compound flavor protease (Flarouzyme produced b...
Embodiment 2
[0037] The difference from Example 1 is that: 1000 kg of sea cucumber boiling liquid was concentrated in a vacuum thermal diaphragm cryogenic concentrator at a vacuum degree of 4.0 MPa and a concentration temperature of 60° C. for 2 hours to concentrate 500 kg of sea cucumber boiling liquid, and the concentration rate was 50%;
[0038] Use a 1000L vacuum bioreactor to adjust the pH to 7, the temperature is 50°C, and the vacuum degree is 200Pa. Add 200g of compound protease; 100g of 1398 neutral protease; 150g of 1203 fat; 100g of Vc, and stir for 8 hours. Separation with a 100-mesh to 300-mesh duplex filter to obtain 500kg of compound enzymolysis solution, modify the reaction kettle with 1000L biological enzymes, adjust the pH value to 7, the temperature is 50°C, and the vacuum degree is 300Pa. Add 400g of compound flavor protease; 50g of phospholipase A; stir the enzyme modification reaction for 4 hours, and inactivate the enzyme at 140°C for 30min.
[0039] Use 2-1um, 0.4-0....
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