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Ultrasonic extraction method for salvianolic acid B

An extraction method and ultrasonic technology are applied in the extraction of salvianolic acid B, and the ultrasonic extraction field of salvianolic acid B can solve the problems of low extraction yield and low extraction efficiency, achieve simple process and avoid low extraction yield. , the effect of short extraction time

Inactive Publication Date: 2011-03-23
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method overcomes the problems of low extraction efficiency and low extraction yield caused by the thermal decomposition of salvianolic acid B in the traditional reflux extraction method, and is suitable for large-scale industrial production of salvianolic acid B

Method used

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  • Ultrasonic extraction method for salvianolic acid B
  • Ultrasonic extraction method for salvianolic acid B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Weigh 100g of Danshen dry medicinal powder with 10 meshes, load it into a 2000ml flask, seal it with a plastic film, add 500ml of 40% ethanol, and extract for 10min at 50°C under ultrasonic waves with a frequency of 30kHz. After the extraction is completed, the crude extract is filtered, and the solvent is recovered to dryness under reduced pressure (control the concentration temperature not to exceed 40° C.) to obtain the crude extract. Then it was dissolved in cold water at 4°C, filtered to the solution, concentrated under reduced pressure to a volume of 10ml, acidified with 10% hydrochloric acid to pH2, and extracted with ethyl acetate 3-8 times. Combine the extracts and evaporate the ethyl acetate to obtain 6.6 g of salvianolic acid B with a purity greater than 40%.

Embodiment 2

[0025] Weigh 100g of dried Danshen medicinal powder of 30 meshes, load it into a 2000ml flask, seal it with a plastic film, add 1000ml of 50% ethanol, and extract for 20min at 20°C under ultrasonic waves with a frequency of 40kHz. After the extraction is completed, the crude extract is filtered, and the solvent is recovered to dryness under reduced pressure (control the concentration temperature not to exceed 40° C.) to obtain the crude extract. Then dissolve in cold water at 4°C, filter and transfer the solution, concentrate under reduced pressure to a volume of 20ml, acidify with 10% hydrochloric acid to pH 3, and take 3-8 times with n-butanol. Combine the extracts and volatilize n-butanol to obtain 6.6 g of salvianolic acid B with a purity greater than 40%.

Embodiment 3

[0027] Weigh 100g of dried Danshen medicinal powder with 50 meshes, load it into a 5000ml flask, seal it with plastic film, add 1500ml of 60% ethanol, and extract 25min at 30°C under ultrasound frequency of 50kHz. After the extraction is completed, the crude extract is filtered, and the solvent is recovered to dryness under reduced pressure (control the concentration temperature not to exceed 40° C.) to obtain the crude extract. Then dissolve in cold water at 4°C, filter the solution, concentrate under reduced pressure to a volume of 30ml, acidify to pH 4 with 10% hydrochloric acid, and extract 3-8 times with ethyl acetate. Combine the extracts and evaporate the ethyl acetate to obtain 6.6 g of salvianolic acid B with a purity greater than 40%.

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Abstract

The invention discloses an ultrasonic extraction method for salvianolic acid B. Dry salvia is crashed into powder of 10 meshes to 80 meshes; ethanol of 40 percent to 80 percent with the volume weight ratio of 5 times to 30 times is added into the powder, and ultrasonic extraction is carried out for 5 min to 40 min under the condition of 0 DEG C to 60 DEG C and 30 k Hz to 80 k Hz to obtain a crudeextract; the crude extract is filtered, concentrated and dried to obtain crude extracted substance. Cold water with the volume weight ratio of 2 times to 5 times is added into the crude extracted substance to be fully and uniformly mixed and filtered; the filter liquor is collected and concentrated in a decompression mode; the concentrated solution is transformed into solution with volume weight ratio of 0.01 to 1 time the dry salvia; the pH value of concentrated solution is regulated with hydrochloric acid of 10 percent to be 2-5, and ethyl acetate or butanol are used to extract the concentrated solution for 3 times to 8 times; the extract solution is combined, organic solvent is evaporated, and the salvianolic acid B is obtained. The method has simple technology, short extraction time, low cost and high efficiency, and is suitable for large-scale industrial production.

Description

Technical field [0001] The invention belongs to the technical field of extraction, separation and purification of natural products, and relates to a method for extracting salvianolic acid B, in particular to an ultrasonic extraction method for salvianolic acid B. Background technique [0002] Salvia miltiorrhiza bunge (Salvia miltiorrhiza bunge) is a plant belonging to the sage of the Lamiaceae family. It is a traditional Chinese herbal medicine. It is clinically used to treat chest and abdomen tingling, heat arthralgia, sore swelling, upset and insomnia, hepatosplenomegaly, and angina. Etc. Salvia contains two major types of compounds, one is fat-soluble tanshinones, and the other is water-soluble salvianolic acids. The phenolic acid compounds of salvia miltiorrhiza have anti-thrombosis effects and have protective effects on brain damage caused by cerebral ischemia and reperfusion in mice (Chen et al., 2000). Among them, salvianolic acid B has strong scavenging free radicals an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/86B01J19/12A61P39/06
Inventor 董娟娥梁宗锁王渭玲
Owner NORTHWEST A & F UNIV
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