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Method of hydrolyzing ligno-cellulose with cellulosic enzyme

A technology of lignocellulose and cellulase, which is applied in the field of lignocellulose hydrolysis and lignocellulose hydrolysis by cellulase, can solve problems such as the decrease of reaction surface renewal speed, protein denaturation, and enzyme failure, and achieve cellulose conversion rate Improve, promote the reaction, enhance the effect of mass transfer

Inactive Publication Date: 2011-03-30
GUANGZHOU CHEM CO LTD CHINESE ACADEMY OF SCI
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  • Summary
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AI Technical Summary

Problems solved by technology

In fact, the adsorption of cellulase on the surface of lignin in the reaction substrate will cause the ineffectiveness of some enzymes. By adding non-ionic surfactants, the ineffective adsorption of enzymes can be inhibited, and the diffusion properties of substances between the two phases can be improved. Increase the reaction rate, but after a period of reaction, the adsorption on the surface of the substrate and the enzyme molecule reaches equilibrium, so that the renewal rate of the reaction surface decreases, and the system is gradually in a relatively inflexible state
The action of ultrasound can quickly remove the remaining solid particles on the reacted surface, renew the reaction surface, enhance mass transfer, and promote the reaction, but the extreme physical and chemical conditions generated by ultrasound can easily lead to protein denaturation and inactivation of enzymes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Crush corn stalks to 60-80 mesh, disperse them in 1.0% NaOH solution at a solid-to-liquid ratio of 1:10 g / mL, cook for 2 hours, filter while hot, wash with water until the pH of the filtrate is 5-7, dry and prepare Enzymatic use.

[0017] Bagasse is crushed to 60-80 mesh, dispersed in 1.0% KOH solution at a solid-to-liquid ratio of 1:10 g / mL, cooked for 3 hours, filtered while hot, washed with water until the pH of the filtrate is 5-7, dried and prepared Enzymatic use.

[0018] Rice straw is crushed to 60-80 mesh, dispersed in 0.9% NaOH solution at a solid-to-liquid ratio of 1:10 g / mL, boiled for 5 hours, filtered while hot, washed with water until the pH of the filtrate is 5-7, dried and prepared Enzymatic use.

Embodiment 2

[0020] Take 40 g / L of lignocellulose obtained by pretreatment of corn stalks, disperse in 0.1 mol / L citric acid-sodium citrate buffer solution with pH 5.0, shake well, add 0.6 g / L cellulase to start hydrolysis. After reacting for 60 hours, the content of total reducing sugar and glucose in the filtrate was measured by DNS method and HPLC. The yield of total reducing sugar was 328mg / g dry material, and the final cellulose conversion rate was 55.8%.

Embodiment 3

[0022] Take 40 g / L of lignocellulose obtained by the pretreatment of corn stalks, disperse it in 0.1 mol / L citric acid-sodium citrate buffer solution with a pH of 5.0, add 2.0 g / L polyethylene glycol 4000, shake well, and let it stand at 50°C. After 2 hours, add 0.6g / L cellulase to start hydrolysis. After reacting for 60 hours, the content of total reducing sugar and glucose in the filtrate was measured by DNS method and HPLC. The yield of total reducing sugar was 398mg / g dry matter, and the final cellulose conversion rate was 72.1%.

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Abstract

The present invention discloses a method of hydrolyzing ligno-cellulose with cellulosic enzyme, which can hydrolyze ligno-cellulose by dispersing ligno-cellulose in citric acid-sodium citrate buffer solution, adding polyethylene glycol and cellulosic enzyme into the buffer solution, and letting the ligno-cellulose to react with the polyethylene glycol under intermittent ultrasonic action; the technical scheme of the present invention overcomes the drawback of enzyme adsorption resulted from adding polyethylene glycol solely and enzyme deactivation resulted from applying ultrasonic wave solely, and can effectively improve the efficiency of ligno-cellulose hydrolysis with the aid of cellulosic enzyme.

Description

technical field [0001] The invention belongs to the field of lignocellulose hydrolysis, especially the field of hydrolyzing lignocellulose by cellulase. Background technique [0002] The shortage of traditional energy has led to the rapid rise of the fuel ethanol industry. The production of fuel ethanol with corn, wheat and other food crops as raw materials can easily lead to food shortages. Therefore, governments of various countries have begun to actively encourage the production of fuels from agricultural waste such as straw, bagasse and other lignocellulosic raw materials. ethanol. This technology mainly obtains products through pretreatment of raw materials, cellulase hydrolysis and alcoholic fermentation. Among them, cellulase hydrolysis of lignocellulose is a key step in this technology, which belongs to a relatively complex heterogeneous biochemical reaction system. The current research hotspots in this field are mainly focused on improving enzyme utilization, main...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B15/02C12S3/04
Inventor 计红果庞浩刘伟廖兵
Owner GUANGZHOU CHEM CO LTD CHINESE ACADEMY OF SCI
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