Method for preparing radix astragali homopolysaccharide

A technology of astragalus and polysaccharides, which is applied in the field of preparation of homopolysaccharides of astragalus, and achieves the effect of simple, fast and low-cost methods

Inactive Publication Date: 2009-10-14
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

No further public literature reports have been found on the preparation method of astragalus polysaccharides with clear molecular weight and clear polysaccharide composition

Method used

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  • Method for preparing radix astragali homopolysaccharide

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Embodiment 1

[0024] Take 500g of astragalus crude powder, add 1500mL 95% ethanol (v / v) to reflux for defatting for 1.5h, filter, add 3000mL water to the residue and reflux extract three times, each time for 90min. The extract was filtered while hot, and the filtrates were combined and concentrated under reduced pressure to 1800 mL. Add 95% ethanol (v / v) to the concentrated solution at 4°C, stir, adjust the concentration of ethanol in the solution to 50% (v / v), and let stand overnight. Centrifuge to collect the precipitate, wash once with absolute ethanol, then wash once with acetone, repeat twice, and dry under vacuum at 60°C to obtain 25g of crude astragalus polysaccharide in the form of brown yellow powder, with an extraction yield of 5%.

[0025] Take 20g of crude polysaccharide to make 20g·L -1 Add 20g of pepsin to 1000mL aqueous solution, enzymatically hydrolyze at 37°C for 3h, boil for 10min to denature and precipitate excess protease, and remove by centrifugation. Add 250 mL of Se...

Embodiment 2

[0032] Take 500g of astragalus crude powder, add 1500mL of 95% ethanol (v / v) to reflux for defatting for 1.5h, filter, add 4500mL of water to the residue and reflux for extraction three times, each time for 90min. The extract was filtered while hot, and the filtrates were combined and concentrated under reduced pressure to 1800 mL. Add 95% ethanol (v / v) to the concentrated solution at 4°C, stir to adjust the alcohol concentration of the solution to 65% (v / v), and let stand overnight. Centrifuge to collect the precipitate, alternately wash twice with absolute ethanol and acetone, and vacuum-dry at 60° C. to obtain 50 g of crude astragalus polysaccharide in brown-yellow powder form, with an extraction yield of 10%.

[0033] Take 20g of crude polysaccharide to make 20g·L -1 Add 60g (m / m) of pepsin to 1000mL aqueous solution, enzymatically hydrolyze for 5h at 37°C, boil for 10min to denature and precipitate excess protease, and remove it by centrifugation. Add 200 mL of Sevage r...

Embodiment 3

[0037] Take 500g of Astragalus crude powder, add 1500mL 95% ethanol (v / v) to reflux for defatting for 1.5h, filter, add 6000mL water to the residue and reflux extract three times, each time for 90min. The extract was filtered while hot, and the filtrates were combined and concentrated under reduced pressure to 1800 mL. Add 95% ethanol (v / v) to the concentrated solution at 4°C, stir to adjust the alcohol concentration of the solution to 80%, and let stand overnight. Centrifuge to collect the precipitate, alternately wash twice with absolute ethanol and acetone, and vacuum-dry at 60° C. to obtain 75 g of crude astragalus polysaccharide in the form of brown yellow powder, with a yield of 15%.

[0038] Take 20g of crude polysaccharide to make 20g·L -1 Add 100g of pepsin to 1000mL aqueous solution, enzymatically hydrolyze at 37°C for 6h, boil for 10min to denature and precipitate excess protease, and remove by centrifugation. 150 mL of Sevage reagent (volume ratio of chloroform:n...

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Abstract

The invention provides a method for preparing radix astragali homopolysaccharide in the technical field of Chinese medicine. The method comprises the following steps that: radix astragali coarse powder is taken, refluxed and degreased, filtered, refluxed and extracted with water, concentrated, precipitated, centrifuged or filtered to collect precipitate, and the precipitate is washed and dried in vacuum to obtain radix astragali coarse polysaccharide; aqueous solution of the radix astragali coarse polysaccharide is enzymatically hydrolyzed with pepsin, boiled and centrifuged to obtain supernatant fluid; a Sevage agent is added to the supernatant fluid for shaking and extraction so as to collect upper layer aqueous phase solution; diethylaminoethyl cellulose is added to be filtered, concentrated, dialyzed, precipitated, centrifuged or filtered, washed and dried to obtain the refined radix astragali homopolysaccharide; the refined radix astragali homopolysaccharide is separated and purified through column chromatography twice, and eluted with water, the eluant is collected, concentrated, refrigerated and dried to obtain the radix astragali homopolysaccharide. The average molecular weight of the radix astragali polysaccharide obtained in the invention is about 9000 and the radix astragali homopolysaccharide only contains glucose monosaccharide unit after a complete acid hydrolysis.

Description

technical field [0001] The invention relates to a preparation method in the technical field of traditional Chinese medicine, in particular to a preparation method of astragalus homopolysaccharide. Background technique [0002] Polysaccharides are a class of natural macromolecular compounds, which are polymers linked by aldose or ketose through glycoside bonds. Since it was discovered in the 1970s that polysaccharides and carbohydrate complexes were involved in the regulation of various life phenomena in cells, people have continuously discovered that polysaccharides have various biological activities such as anti-tumor, immune regulation, hypoglycemic, anti-viral, hypolipidemic, anti-coagulation, etc. . And because polysaccharides have no obvious toxic and side effects, the development, utilization and research of polysaccharide biological resources have become increasingly active, and have become a research hotspot in natural medicine, biochemistry, and life sciences. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 赵爱华贾伟牟鲁霞
Owner SHANGHAI JIAO TONG UNIV
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