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Technique for producing 2-keto-D-gluconic acid by fermentation of gluconate

A gluconate and gluconic acid technology, applied in fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of complex medium formula and long fermentation period, and achieve easy extraction and purification, short fermentation period, and shortened fermentation period. Effect

Active Publication Date: 2009-10-14
郑州拓洋生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of technology of gluconate fermentative production 2-keto-D-gluconic acid, to overcome the problems such as traditional method culture medium formula is complicated, fermentation period is long etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Seed cultivation adopts 5L automatic control fermenter, and the formula of seed culture medium is: glucose 2% (weight volume percentage, the same below), corn steep liquor 1%, KH 2 PO 4 0.2%, Urea 0.2%, MgSO 4 ·7H 2 O 0.05%, bubble enemy 0.05%; pH 6.8 before sterilization. Introduce the Pseudomonas fluorescens K1005 suspension into about 3L of the above seed medium, control the stirring speed at 400r / m, the tank temperature at 33.5°C, the tank pressure at 0.04MPa, and the ventilation rate at 0.5V / V / m, and cultivate for 18h.

[0026] Fermentation adopts 50L self-control fermentation tank, and the formula of fermentation medium is as follows: calcium gluconate 20% (wherein 40% is used as base material, 60% is used as supplementary material), corn steep liquor 1%, bubble enemy 0.05%; pH6.5 before sterilization. The base material calcium gluconate solution and corn steep liquor were sterilized at 120°C for 30 minutes, and mixed at 38°C, and the above-mentioned cultured ...

Embodiment 2

[0028] Seed culture is the same as in Example 1.

[0029] Fermentation adopts 50L automatic control fermenter, and fermentation medium formula is as follows: sodium gluconate 20% (wherein 40% is used as base material, 60% is used as supplementary material), corn steep liquor 1%, light CaCO 3 5.5%, foam enemy 0.03%, pH 6.3 before sterilization, base material sodium gluconate, light CaCO 3 and corn steep liquor were sterilized at 120°C for 30 minutes, and mixed at 40°C, and the cultured seed solution was added to 20L fermentation medium, and sodium gluconate as a supplement was made into a 10L solution for disinfection, and then added by feeding method , it is advisable to control the flow speed to maintain the sodium gluconate content in the fermentation broth at about 2%. Control the stirring speed at 360r / m, the tank temperature at 30°C, the tank pressure at 0.03MPa, the ventilation rate at 1.5v / v / m, and cultivate for 36h. 4996.1 g of 2-keto-D-gluconic acid was produced, an...

Embodiment 3

[0031] Seed culture is the same as in Example 1, and the strain is Arthrobacter globosa K1022.

[0032] Fermentation adopts 50L self-control fermenter, and fermentation medium formula is as follows: calcium gluconate 18% (wherein 40% is as base material, 60% is as supplementary material), corn steep liquor 1%, bubble enemy 0.06%, pH 6.4 before sterilization, will The base material calcium gluconate solution and corn steep liquor were sterilized at 120°C for 30 minutes, and mixed at 41°C. The cultured seed solution was added to about 20L of fermentation medium, and calcium gluconate as supplement was made into 10L After the solution has been eliminated, it is added in a fed-batch manner, and it is advisable to control the flow velocity to maintain the calcium gluconate content in the fermentation broth at about 2%. Control the stirring speed at 380r / m, the tank temperature at 32°C, the tank pressure at 0.02MPa, the ventilation rate at 0.5v / v / m, and cultivate for 34h. 5042.6 g ...

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PUM

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Abstract

The invention pertains to the field of preparing the salt synthetic precursor of a food additive, namely, 2-keto-D-gluconic acid, in particular to a technique for producing the 2-keto-D-gluconic acid by fermentation of gluconate; wherein the gluconate is used as the carbon source in a fermentation medium for fermentation culture. The invention has simple formulation of fermentation medium, short fermentation period, lower cost, no need of adding of buffer, and less impurity, and is convenient for extraction and purification.

Description

(1) Technical field [0001] The invention belongs to the field of preparation of salt synthetic precursor 2-keto-D-gluconic acid of food additives, in particular to a process for producing 2-keto-D-gluconic acid by fermentation of gluconate. (2) Background technology [0002] 2-Keto-D-gluconic acid is one of the organic acids produced on a large scale in the industry at present, and it is the precursor for the synthesis of food antioxidant sodium D-erythorbate and D-erythorbic acid. The production methods of 2-keto-D-gluconic acid mainly include enzymatic method, chemical synthesis method and fermentation method. [0003] At present, in industrial production, 2-keto-D-gluconic acid is produced by fermentation, using D-glucose as raw material. The method has the following disadvantages: the formula of the medium is complicated, and the fermentation period is long; in addition, a large amount of CaCO needs to be added during the fermentation process. 3 etc. as a pH buffering ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/58C12R1/39C12R1/06
Inventor 闫世梁王敬臣黄惠英史小利刘银霞张生克王新华董明朱腾跃
Owner 郑州拓洋生物工程有限公司
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