Method for transiting cells through electrostatic adherence

An electrostatic adsorption, cell technology, applied in biochemical equipment and methods, preparation of samples for testing, determination/inspection of microorganisms, etc. question

Inactive Publication Date: 2009-10-14
孝感宏翔生物医械技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Cell adsorption and transfer is an important step in the production process. Whether the cells adsorbed by the filter membrane can be effectively transferred to the glass slide is the key to the quality of the production. Usually, due to the contact between the two objects, the filter The cells on the membrane cannot be effectively transferred to the glass slide, resulting in incomplete transfer, and a large number of cells are lost. At present, the common technology adopts the method of heating the glass slide to solve the problem. A heating device is installed on the slide fixing clip, and the slide is placed After going up, the glass slide is heated through heat conduction. When the cells are transferred, the cells are transferred to the glass slide due to the contact of hot and cold objects.
[0003] The problem with this method is that the cells are deformed by heat, and the cells, cytoplasm, and nucleus are deformed and damaged by heat, which brings problems to medical diagnosis, makes diagnosis difficult, and is prone to misdiagnosis and misdiagnosis.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0005] The way to realize the present invention is to use chemical method to process the glass slide and preservation solution.

[0006] The slides are pre-treated by chemical methods. First, the slides are soaked with chemical reagents, and an appropriate amount of ferric chloride is added to the reagent mixture. After soaking the slides, the slides will be coated with Fe(OH) 3 The coating of the composition, it is a kind of electrolyte, the colloid can generate static electricity on the glass slide, and has the function of adsorbing ions. The glass slide is air-dried and packaged. When it comes into contact with the liquid during use, it has the function of adsorbing the cells contained in the liquid. .

[0007] For the formula of the preservation solution, 3% methanol is used to preserve the cells. At the same time, alkoxide ions (CHO-) and hydrogen ions (H+) are generated in the preservation solution. When the cells enter the preservation solution, they are fully stirred. ...

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PUM

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Abstract

The invention relates to a method for transiting cells through electrostatic adherence. The method is used when a cell, in particular a liquid-based thin-layer cell, is collected and made into a slide during the clinical pathology examination. The method is characterized in that the liquid-based thin-layer cell has an alcohol ion (CHO) and a hydrogen ion (H), and the slide has ferric trichloride (Fe(OH)3). During cell transition, because the cell has the alcohol ion (CHO) and the hydrogen ion (H), the cell is easily adhered by the (Fe(OH)3) on the slide so that the process of transiting a great number of cells from a filtering membrane to a slide can be smoothly realized. The invention prevents the insufficient cell transition, and the cell is not deformed, therefore, the aims of high quality slide making and accurate judgment can be achieved.

Description

technical field [0001] The invention relates to a method for transferring cells by electrostatic adsorption used in clinical pathological examination for cell collection and preparation of cells, especially liquid-based thin-layer cells. Background technique [0002] Cell adsorption and transfer is an important step in the production process. Whether the cells adsorbed by the filter membrane can be effectively transferred to the glass slide is the key to the quality of the production. Usually, due to the contact between the two objects, the filter The cells on the membrane cannot be effectively transferred to the glass slide, resulting in incomplete transfer, and a large number of cells are lost. At present, the common technology adopts the method of heating the glass slide to solve the problem. A heating device is installed on the slide fixing clip, and the slide is placed After going up, the glass slide is heated through heat conduction. When the cells are transferred, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/36G01N33/48C12Q1/02
Inventor 沈红安
Owner 孝感宏翔生物医械技术有限公司
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