Anti-tumor pharmaceutical composition and preparation method thereof
A production method and composition technology, which are applied in the field of biopharmaceuticals, can solve the problems of not very obvious effects, and achieve the effects of reasonable design, high extraction purity, and simple and convenient operation.
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[0032] Example 1:
[0033] (1) Preparation of Antrodia cinnamomea extract: take the Antrodia cinnamomea crude powder, add 6 times the weight of the Antrodia cinnamomea crude powder with ethanol with a concentration of 75%, extract at a temperature of 75°C for 2 hours, and then filter; take the filter residue after filtration and add it to the filter residue Add 75% ethanol with a concentration of 4 times the weight of the filter residue, extract for 1.5 hours at a temperature of 75°C, and filter; combine the two filtrates, reflux and condense the ethanol with an ethanol reflux tower to recover ethanol, until there is no alcohol taste, to obtain an extract; Add water 22 times the weight of the filter residue to the filter residue after the second filtration, and filter it after 2 hours of extraction; take the filter residue after filtration, add 10 times the weight of the filter residue to the filter residue, and filter after 1.5 hours of extraction; combine twice After the filtrat...
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[0037] Experimental example:
[0038] 1. Drug evaluation index: tumor inhibition rate (%).
[0039]
[0040] Observation time: 14 days.
[0041] 2. The main steps of the experiment:
[0042] 2.1 Tumor strains and solid tumor transplantation methods: Mouse liver cancer (H22) tumor strains are routinely preserved by the Central Laboratory of the Cancer Institute of the Chinese Academy of Medical Sciences. The tumor cells were taken out of liquid nitrogen, quickly thawed at 37°C and resuscitated, and then inoculated into the abdominal cavity of the mouse. Eight days later, the abdominal swelling of the mouse was seen, and the tumor was successfully resuscitated.
[0043] 2.2 Tumor inoculation and grouping: the tumor-bearing mice were sacrificed and the ascites cancer cells were extracted under aseptic conditions, diluted, counted, and each mouse was inoculated with 0.2 ml of cell suspension. They were randomly divided into 6 groups with 10 animals in each group. Namely the normal s...
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[0057] Example 2:
[0058] (1) Preparation of Antrodia cinnamomea extract: Take the Antrodia cinnamomea crude powder, add 10 times the weight of the Antrodia cinnamomea crude powder in ethanol with a concentration of 60%, and extract at a temperature of 60°C for 3 hours, then filter; take the filter residue after filtration and add it to the filter residue Add ethanol with a concentration of 60% 5 times the weight of the filter residue, extract at a temperature of 65°C for 2 hours, and then filter; combine the two filtrates, reflux and condense the ethanol with an ethanol reflux tower to recover ethanol until there is no alcohol taste, to obtain an extract; Add water 15 times the weight of the filter residue to the filter residue after the second filtration, and filter it after 3 hours of extraction; take the filter residue after filtration, add 15 times the weight of the filter residue to the filter residue, and filter after 3 hours of extraction; combine twice After the filtrate...
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