Anti-tumor pharmaceutical composition and preparation method thereof

A production method and composition technology, which are applied in the field of biopharmaceuticals, can solve the problems of not very obvious effects, and achieve the effects of reasonable design, high extraction purity, and simple and convenient operation.

Active Publication Date: 2009-10-21
ZHEJIANG FANGGE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also some drugs on the market that enhance the body's own immunity, b

Method used

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  • Anti-tumor pharmaceutical composition and preparation method thereof
  • Anti-tumor pharmaceutical composition and preparation method thereof
  • Anti-tumor pharmaceutical composition and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] Example 1:

[0033] (1) Preparation of Antrodia cinnamomea extract: take the Antrodia cinnamomea crude powder, add 6 times the weight of the Antrodia cinnamomea crude powder with ethanol with a concentration of 75%, extract at a temperature of 75°C for 2 hours, and then filter; take the filter residue after filtration and add it to the filter residue Add 75% ethanol with a concentration of 4 times the weight of the filter residue, extract for 1.5 hours at a temperature of 75°C, and filter; combine the two filtrates, reflux and condense the ethanol with an ethanol reflux tower to recover ethanol, until there is no alcohol taste, to obtain an extract; Add water 22 times the weight of the filter residue to the filter residue after the second filtration, and filter it after 2 hours of extraction; take the filter residue after filtration, add 10 times the weight of the filter residue to the filter residue, and filter after 1.5 hours of extraction; combine twice After the filtrat...

Example Embodiment

[0037] Experimental example:

[0038] 1. Drug evaluation index: tumor inhibition rate (%).

[0039]

[0040] Observation time: 14 days.

[0041] 2. The main steps of the experiment:

[0042] 2.1 Tumor strains and solid tumor transplantation methods: Mouse liver cancer (H22) tumor strains are routinely preserved by the Central Laboratory of the Cancer Institute of the Chinese Academy of Medical Sciences. The tumor cells were taken out of liquid nitrogen, quickly thawed at 37°C and resuscitated, and then inoculated into the abdominal cavity of the mouse. Eight days later, the abdominal swelling of the mouse was seen, and the tumor was successfully resuscitated.

[0043] 2.2 Tumor inoculation and grouping: the tumor-bearing mice were sacrificed and the ascites cancer cells were extracted under aseptic conditions, diluted, counted, and each mouse was inoculated with 0.2 ml of cell suspension. They were randomly divided into 6 groups with 10 animals in each group. Namely the normal s...

Example Embodiment

[0057] Example 2:

[0058] (1) Preparation of Antrodia cinnamomea extract: Take the Antrodia cinnamomea crude powder, add 10 times the weight of the Antrodia cinnamomea crude powder in ethanol with a concentration of 60%, and extract at a temperature of 60°C for 3 hours, then filter; take the filter residue after filtration and add it to the filter residue Add ethanol with a concentration of 60% 5 times the weight of the filter residue, extract at a temperature of 65°C for 2 hours, and then filter; combine the two filtrates, reflux and condense the ethanol with an ethanol reflux tower to recover ethanol until there is no alcohol taste, to obtain an extract; Add water 15 times the weight of the filter residue to the filter residue after the second filtration, and filter it after 3 hours of extraction; take the filter residue after filtration, add 15 times the weight of the filter residue to the filter residue, and filter after 3 hours of extraction; combine twice After the filtrate...

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Abstract

The invention relates to an anti-tumor pharmaceutical composition and a preparation method thereof, and belongs to the technical field of bio-pharmaceuticals. The pharmaceutical composition comprises the following components: 20 to 60 percent of extract of brown heart rot of cinnamomum kanehirai, 5 to 30 percent of glossy ganoderma extract and 20 to 60 percent of grifola frondosa extract. The preparation method comprises the following steps: preparing the extract of the brown heart rot of the cinnamomum kanehirai; preparing the glossy ganoderma extract; preparing the grifola frondosa extract; and mixing the extract of the brown heart rot of the cinnamomum kanehira, the glossy ganoderma extract and the grifola frondosa extract. The anti-tumor pharmaceutical composition is an immunopotentiator, has no toxic and side effect, and mainly achieves the anti-tumor effect by reinforcing the immunological competence of the human body. The anti-tumor pharmaceutical composition has reasonable compatibility, and all the components are from natural products, so that the anti-tumor pharmaceutical composition is safe and has no toxic and side effect. The tumor inhibition rate of the anti-tumor pharmaceutical composition is 26.16 percent (P is less than 0.05) and is equivalent to that of the prior anti-cancer medicine cyclophosphamide, but the toxicity of the anti-tumor pharmaceutical composition is obviously lower than that of the cyclophosphamide. The preparation method for the anti-tumor pharmaceutical composition has reasonable design, simple and convenient operation and high extraction purity.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to an antitumor pharmaceutical composition and a production method thereof. Background technique [0002] Antrodia cinnamomea is also called Antrodia cinnamomea or Antrodia camphorata, and its scientific name is Antrodia Camphorata. [0003] Antrodia camphorata is a unique fungus in Taiwan, which only grows on the unique camphor tree in Taiwan. Antrodia Cinnamomea grows in the decayed hollow interior of the trunk of Cinnamomum camphora or on the moist surface of a fallen trunk. The wild Antrodia camphorata grows on old camphor trees. The fruiting bodies vary in shape, such as plate-shaped, bell-shaped, horseshoe-shaped or tower-shaped; they are bright red when they are born, and gradually become white, light reddish brown, light brown or light yellowish brown. The aroma of Antrodia camphorata is hard and flat. It has the effects of dispelling wind and promo...

Claims

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Application Information

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IPC IPC(8): A61K36/074A61P35/00
Inventor 徐财泉茆广华周晓芬
Owner ZHEJIANG FANGGE PHARMA
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